Histomorphometric analysis of adult articular calcified cartilage zone

Wang, Fuyou; Zhang, Ying; Duan, Xiaojun; Tan, Huiwen; Yang, Bin; Guo, Lin; Chen, Guangxing; Dai, Gang; Ma, Zhe; Yang, Liu

doi:10.1016/j.jsb.2009.08.010

Cited by 64 publications

(82 citation statements)

References 20 publications

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“…The subchondral bone was anchored tightly with cartilage in "comb-anchor" [11] or interdigitations to reduce stress concentrations [12], our previous study also confirmed the presence of gomphosis and hole like structure on native subchondral bone plate [13,14]. However, interface structure was seldom considered in available biphasic osteochondral study.…”

Section: Introductionmentioning

confidence: 62%

The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing

Zhang

Lian

et al. 2015

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 62%

The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing

Zhang

Lian

et al. 2015

Materials Science and Engineering: C

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“…[11][12][13] Recently, the present authors succeeded in imaging of HDL deposited in coronary artery in patients, 26) as in the case of imaging of oxLDL using Evans blue dye. 17,27) Study limitations: The present fl uorescent microscopic study using FG as a biomarker appears to be the fi rst of its kind to image native HDL in the human vascular wall.…”

Section: Discussionmentioning

confidence: 96%

“…We recently discovered that Fast green dye (FG), which is used clinically to stain oral mucosal micronuclei for detection of the genotoxicity of antihyperglycemic drugs in patients with diabetes mellitus, 11) intraocular membrane staining, 12) and subchondral bone staining, 13) elicits a brown fl uorescent color from HDL when fl uorescence is excited at 420 ± 20-nm and emitted at 515-nm light wavelengths. Therefore, in the present study, color fluorescent microscopic (CFM) scanning of the transected surface of the excised human coronary artery (both plaques and normal segments) was performed to clarify the localization of native HDL using FG as a biomarker, and to correlate it to plaque morphology studied by conventional angioscopy.…”

Section: A High Plasma Level Of High-density Lipoprotein (Hdl)mentioning

confidence: 99%

Localization of Native High-Density Lipoprotein and Its Relation to Plaque Morphology in Human Coronary Artery

Uchida¹,

Hiruta

Uchida

et al. 2013

Int. Heart J.

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SummaryHigh-density lipoprotein (HDL) plays a key role in reverse cholesterol transport, and halts the progression of atherosclerosis. However, its localization in human vascular wall is not well understood. We discovered that by exciting at 470-nm and emitting at 515-nm light wavelengths, Fast green dye (FG) elicits brown fl uorescence characteristic of HDL only. Therefore, the localization of native HDL in normal segments and plaques in excised human coronary artery was investigated by scanning their transected surface with color fl uorescent microscopy (CFM) using FG as a biomarker, and the relationships between the localization of HDL and morphology of plaques and normal segments classifi ed by conventional angioscopy and histology were examined. The % incidence of HDL in 13 normal segments (NS) with thin is associated with decreased risk of coronary heart disease. 1) Elevating the plasma level of HDL by fibrates is associated with a lower incidence of coronary heart disease.2) Mechanisms by which antiatherogenic HDL reduces atherosclerosis include facilitating cholesterol uptake from cholesterol-loaded macrophage-foam cells in plaques for transport back to the liver, 3,4) a role as an anti-infl ammatory lipoprotein, decreasing oxidized low-density lipoprotein (oxLDL), increasing nitric oxide synthesis, 5) improving endothelial function, and as an antithrombotic agent. 5) Infusions of HDL or its major component, apolipoprotein A-1 (Apo A-1), halt the progression or induce the regression of atherosclerosis. [6][7][8] The relationship between the plasma HDL levels and atherosclerotic lesions has been extensively investigated. Traffi cking HDL movement was performed using gadolinium methianethiosulfonate-labeled ApoA-1 in animal liver and kidney, 9) and using nanocrystals such as Au-HDL and FeO-HDL in the mouse aorta, 10) but imaging of native HDL in the human vascular wall has not been successful to date. Therefore, it is not known in what type(s) of atherosclerotic plaques native HDL deposits, whether this substance deposits in the normal vascular wall, how this substance acts against atherosclerotic plaque growth and destabilization, and whether or not its concentration changes in parallel with that in the plasma. If HDL could be visualized in the vascular wall, the molecular mechanisms by which HDL causes regression of atherosclerosis and whether or not the amount of HDL in the vascular wall is increased by HDL-raising compounds could be clarified more objectively.We recently discovered that Fast green dye (FG), which is used clinically to stain oral mucosal micronuclei for detection of the genotoxicity of antihyperglycemic drugs in patients with diabetes mellitus, 11) intraocular membrane staining, 12) and subchondral bone staining, 13) elicits a brown fl uorescent color from HDL when fl uorescence is excited at 420 ± 20-nm and emitted at 515-nm light wavelengths. Therefore, in the present study, color fluorescent microscopic (CFM) scanning of the transected surface of the excised human coronary artery (bo...

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“…Anatomically, articular cartilage consists of spatially identifiable four layers, which are commonly named as superficial layer, intermediate layer, deep layer, and calcified layer [9,11]. These layers constitute a complex organization that has layer-dependent alternations in compositions, structures, and properties, and moreover, these alternations are quite irregular in direction [5,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

“…These layers constitute a complex organization that has layer-dependent alternations in compositions, structures, and properties, and moreover, these alternations are quite irregular in direction [5,8,9,10]. Among these layers, the calcified layer acts as a special transition zone that tightly connects the hyaline cartilage and the subchondral bone, and meanwhile, functions as a vital physical barrier to prevent vascular invasion and the calcification of hyaline cartilage [9,10,11]. In addition, the calcified layer has a semi-permeable characteristic and it allows for some molecules with limited sizes to pass through to maintain a stable microenvironment for the survival of chondrocytes [10,11].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix

Liu

Fang

et al. 2018

IJMS

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Chitosan (CH), silk fibroin (SF), and hydroxyapatite (HA) were used to prepare CH/SF/HA composites and the resulting composites were electrospun into nanofibrous membrane units with gradient compositional and structural features. The optimal membrane unit was used together with CH/HA and CH/SF composites to fabricate a type of three-layer scaffold that is intended for osteochondral repair. The bottom layer of the scaffold was built with CH/HA composites and it served as a subchondral layer, the integrated nanofibrous membrane unit functioned as the middle layer for mimicking the calcified layer and the top layer was constructed using CH/SF composites for acting as a chondral layer. The nanofibrous membrane unit was found to be permeable to some molecules with limited molecular weight and was able to prevent the seeded cells from migrating cross the unit, functioning approximately like the calcified layer in the osteochondral matrix. Layered scaffolds showed abilities to promote the growth of both chondrocytes and osteoblasts that were seeded in their chondral layer and bony layer, respectively, and they were also able to support the phenotype preservation of seeded chondrocytes and the mineralization of neotissue in the bony layer. Results suggest that this type of layered scaffolds can function as an analogue of the osteochondral matrix and it has potential in osteochondral repair.

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Histomorphometric analysis of adult articular calcified cartilage zone

Cited by 64 publications

References 20 publications

The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing

The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing

Localization of Native High-Density Lipoprotein and Its Relation to Plaque Morphology in Human Coronary Artery

Chitosan-Based Nanofibrous Membrane Unit with Gradient Compositional and Structural Features for Mimicking Calcified Layer in Osteochondral Matrix

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