Histomorphometric Analysis of Age-Related Changes in Epithelial Thickness and Langerhans Cell Density of the Human Tongue.

Sasaki, Masaru

doi:10.1620/tjem.173.321

Cited by 20 publications

(10 citation statements)

References 45 publications

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“…Hence, it is reasonable to suggest that aging affects the maturation process of oral epithelium, but that the cell proliferation rate increase should not be considered to be the source of this change. Sasaki 17) examined human autopsy findings and found a similar relationship between reduced epithelial thickness and aging. Our findings corroborate his results: that aging is responsible for a morphological change in epithelium through a mechanism that is independent from changes in cell proliferation rate.…”

Section: Discussionmentioning

confidence: 86%

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Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Carrard

Pires

Badauy

et al. 2008

Bull. Tokyo Dent. Coll.

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The aim of this study was to investigate cell proliferation rate and certain morphological features of mouse epithelium as aging progresses. Tongue biopsies were performed on female mice (Mus domesticus domesticus) at 2, 8, 14 and 20 months of age as indicative of adolescence, adulthood, early senescence and senescence, respectively. Histological sections of tongue were stained with hematoxylin-eosin and subjected to silver staining for active nucleolar organizer region counting. Cell proliferation rate and epithelial thickness analysis were carried out. Analysis of variance detected no differences between the groups in terms of numbers of silver-stained dots associated with nucleolar proteins. There was an increase in mean epithelial thickness in adult animals, followed by a gradual reduction until senescence. Mean keratin thickness presented an increase at 8 and 20 months of age. This difference is probably related to puberty, growth or dietary habits. Aging has no influence on oral epithelial proliferation rate in mice. A gradual reduction in epithelial thickness is a feature of aging in mammals. A conspicuous increase in the keratin layer was observed in senescence as an adaptative response to the reduction in epithelial thickness. These results suggest that aging affects the oral epithelium maturation process through a mechanism that is not related to cell proliferation.

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Section: Discussionmentioning

confidence: 86%

“…A single population was followed up, making this a longitudinal study and minimizing the chances of individual characteristics influencing the results. Many studies have evaluated two experimental times, one in adolescence and the other in senescence 6,15,17,19,24) . This could generate misunderstandings, as observed in this study.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Carrard

Pires

Badauy

et al. 2008

Bull. Tokyo Dent. Coll.

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“…Dendritic-shaped cells observed by in vivo confocal microscopy are probably APCs, active in the immunity of the ocular surface, and in vivo LSCM is a useful method for evaluating epithelial DCs distribution on the ocular surface 15. The age dependence of DC density has been shown in both the skin16 17 and the tongue 18. Steuhl19 further demonstrated a decline in the density of DCs with ageing in an ex vivo experiment.…”

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confidence: 97%

Age-related changes of human conjunctiva on in vivo confocal microscopy

Zhu

Hong

Zheng

et al. 2010

British Journal of Ophthalmology

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“…This change was seen more in female than male individuals. Sasaki 16 has analysed the effect of the ageing on the human tongue and following measurement with a computed image‐processing system, the epithelial thickness in older individuals was found to be significantly thinner than in younger ones. However, all of these effects could be associated with the cumulative effects of systemic diseases and/or drugs rather than ageing 17 .…”

Section: Discussionmentioning

confidence: 99%

Exfoliative cytology of the oral mucosa in burning mouth syndrome: a cytomorphological and cytomorphometric analysis

et al. 2011

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doi:10.1111/j.1741‐2358.2009.00319.x   Exfoliative cytology of the oral mucosa in burning mouth syndrome: a cytomorphological and cytomorphometric analysis Objective: The aim of this study was to evaluate oral epithelial cells by exfoliative cytology in burning mouth syndrome (BMS). Material and methods: Oral smears were collected from clinically normal‐appearing mucosa by liquid‐based exfoliative cytology in 40 individuals (20 BMS patients and 20 healthy controls matched for age and gender) and analysed for cytological and cytomorphometric techniques. Results: Mean values of nuclear area (NA) for experimental and control groups were, respectively, 67.52 and 55.64 μm2 (p < 0.05). Cytoplasmic area (CA) showed the following mean values: 1258.0 (experimental) and 2069.0 μm2 (control). Nucleus‐to‐cytoplasm area ratio for the experimental group was 0.07, besides the control group was 0.03 (p < 0.05). Morphologically, oral smears exhibited normal epithelial cells in both experimental and control groups. There was a significant predominance of nucleated cells of the superficial layer in the smears of BMS patients (p = 0.00001). Conclusion: This study revealed that oral mucosa of BMS patients exhibited significant cytomorphometric changes in the oral epithelial cells. These changes probably are associated with epithelial atrophy and a deregulated maturation process that may contribute to the oral symptoms of pain and discomfort in BMS.

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Histomorphometric Analysis of Age-Related Changes in Epithelial Thickness and Langerhans Cell Density of the Human Tongue.

Cited by 20 publications

References 45 publications

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Age-related changes of human conjunctiva on in vivo confocal microscopy

Exfoliative cytology of the oral mucosa in burning mouth syndrome: a cytomorphological and cytomorphometric analysis

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