Histomorphometric and immunohistochemical study shows that tricalcium silicate cement associated with zirconium oxide or niobium oxide is a promising material in the periodontal tissue repair of rat molars with perforated pulp chamber floors

Abstract

Barbosa DD, Delfino MM, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Sasso-Cerri E, Silva GF, Cerri PS. Histomorphometric and immunohistochemical study shows that tricalcium silicate cement associated with zirconium oxide or niobium oxide is a promising material in the periodontal tissue repair of rat molars with perforated pulp chamber floors.

Biocompatibility and bioactive potential of an experimental tricalcium silicate‐based cement in comparison with Bio‐C repair and MTA Repair HP materials

“…In addition, the pure TCS provided a greater deposition of hydroxyapatite after immersion in physiological solution than the PC (Camilleri, 2011). Thus, experimental TCS materials have been proposed as alternative materials to the WMTA‐Angelus (Barbosa et al, 2021; Camilleri et al, 2013; Camilleri, Grech, et al, 2014; Grech et al, 2013; Queiroz, Torres, Rodrigues, Viola, Bosso‐Martelo, Chavez‐Andrade, Guerreiro‐Tanomaru, & Tanomaru‐Filho, 2021; Queiroz, Torres, Rodrigues, Viola, Bosso‐Martelo, Chavez‐Andrade, Souza, et al, 2021; Taha et al, 2021). Several agents, such as niobium oxide, zirconium oxide and calcium tungstate, have provided adequate radiopacity to the calcium silicate‐based materials for use as endodontic repair filling material (Barbosa et al, 2021; Bosso‐Martelo et al, 2016; Queiroz, Torres, Rodrigues, Viola, Bosso‐Martelo, Chavez‐Andrade, Guerreiro‐Tanomaru, & Tanomaru‐Filho, 2021; Silva et al, 2014, 2015).…”

Bioactive potential of Bio‐C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue

“…At 7, 15, and 30 days, the number of IL‐6‐immunolabeled cells was significantly greater around Bio‐C Pulpo and Biodentine specimens than in WMTA specimens. However, it is important to emphasize that IL‐6 is a pro‐inflammatory cytokine detected in endothelial cells, fibroblasts and inflammatory cells 27,38,53 . In the present study, immunoexpression of IL‐6 was computed in different cell types (inflammatory cells, endothelial cells and fibroblasts).…”

“…54,55 Several studies have demonstrated that IL-6 is responsible for the maintenance of inflammatory reaction and, the reduction of this cytokine is associated with tissue connective remodeling, allowing the formation of extracellular matrix components and fibroblasts proliferation. 27,36,38,53,56 In fact, no significant difference in the number of IL-6-immunostained cells and inflammatory cells was observed among the groups, including in the CG specimens, at 60 days, indicating that bioceramic materials are biocompatible.…”

“…Despite the highest values of IL‐6‐immunostained cells detected in Bio‐C Pulpo and Biodentine at 7, 15, and 30 days, the significant reduction in the immunoexpression of this cytokine in these groups over time points to a regression in the inflammatory reaction since IL‐6 plays an important role in the recruitment of inflammatory cells from the bloodstream 54,55 . Several studies have demonstrated that IL‐6 is responsible for the maintenance of inflammatory reaction and, the reduction of this cytokine is associated with tissue connective remodeling, allowing the formation of extracellular matrix components and fibroblasts proliferation 27,36,38,53,56 . In fact, no significant difference in the number of IL‐6‐immunostained cells and inflammatory cells was observed among the groups, including in the CG specimens, at 60 days, indicating that bioceramic materials are biocompatible.…”

“…However, it is important to emphasize that IL-6 is a pro-inflammatory cytokine detected in endothelial cells, fibroblasts and inflammatory cells. 27,38,53 In the present study, immunoexpression of IL-6 was computed in different cell types (inflammatory cells, endothelial cells and fibroblasts). From an in vitro study that evaluated the cell viability of Saos-2 using the MTT method after 24 hours of exposure to the Biodentine extracts showed that this material was cytocompatible in dilutions greater than 1:8, 1:16, and 1:32, being cytotoxic in the initial dilutions of 1:1, 1:2, and 1:4 when compared to the control samples.…”