Histomorphometric and Microbiological Assessment of Photodynamic Therapy as an Adjuvant Treatment for Periodontitis: A Short-Term Evaluation of Inflammatory Periodontal Conditions and Bacterial Reduction in a Rat Model

Prates, Renato Araújo; Yamada, Aécio M.; Suzuki, Luis C.; França, Cristiane Miranda; Cai, Silvana; Mayer, Márcia Pinto Alves; Ribeiro, A. S.; Ribeiro, Martha S.

doi:10.1089/pho.2010.2984

Cited by 46 publications

(27 citation statements)

References 43 publications

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“…After intraperitoneal anesthesia (ketamine chlorohydrate, 0.08 mL/100g body weight; xylazine chlorohydrate, 0.04 mL/100g), a thread of sterile 3-0 black silk (Johnson and Johnson) was placed (on both sides) around the cervix of each upper first molar and knotted mesially (13). The thread remained fully attached in a subgingival position in the cervical region.…”

Section: Ligature-induced Periodontal Diseasementioning

confidence: 99%

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

et al. 2017

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The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-β1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.

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Section: Ligature-induced Periodontal Diseasementioning

confidence: 99%

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

et al. 2017

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“…The small radius of the effect of the cytotoxic chemically reactive molecules which are bactericidal yet safe for the host tissues makes PDT an exciting non‐invasive antimicrobial therapy with little probability of developing resistance . Animal studies have demonstrated absence of any toxicity for the surrounding host tissues including the healing connective tissues …”

Section: Introductionmentioning

confidence: 99%

Effect of photodynamic therapy adjunct to scaling and root planing in periodontitis patients: A randomized clinical trial

Pulikkotil

Toh

Mohandas

et al. 2016

Australian Dental Journal

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“…Antimicrobial PDT combines a nontoxic photoactivatable dye or photosensitizer with harmless visible light of the correct wavelength to excite the dye to its reactive triplet state, which will then generate reactive oxygen species (ROS) such as singlet oxygen and hydroxyl radicals that are toxic to cells (9)(10)(11)(12). aPDT is an emerging alternative to treat localized infections (13)(14)(15), including oral diseases (16) such as periodontitis (13,(17)(18)(19), adjuvant approach for endodontic treatment (20,21) and caries (22,23).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy as a Strategy to Arrest Enamel Demineralization: A Short‐Term Study on Incipient Caries in a Rat Model^†

Baptista

Kato

Prates

et al. 2012

Photochem & Photobiology

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In this study we developed a rat model of incipient caries to investigate the short-term effects of antimicrobial photodynamic therapy (aPDT) on oral microbiota regulation and demineralization arrestment. Twenty-nine male rats were submitted to caries induction. Early carious lesion was confirmed by optical coherence tomography (OCT) 5 days after experiment beginning in five animals. The remaining animals (n = 24) were randomly divided into two groups: control (n = 12), animals were untreated; and aPDT (n = 12), animals were treated with 100 μM of methylene blue for 5 min and irradiated by a light emitting diode at λ = 645 ± 30 nm, fluence rate of 480 mW cm(-2) and exposure time of 3 min. Bacterial burden was evaluated before, immediately after, 3, 7 and 10 days following treatment, and total number of microaerophilic bacteria was counted. OCT was also used to quantify teeth demineralization. A significant bacterial decrease of about 1.6 log was observed immediately after aPDT. Besides, bacterial load in aPDT group remained lower than control until 10 days post-treatment (P < 0.05) and variation of optical attenuation coefficient before and after aPDT was 15%, corroborating to caries arrestment. Put together, these findings suggest that aPDT was competent to reduce cariogenic bacteria and to avoid further mineral loss.

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Histomorphometric and Microbiological Assessment of Photodynamic Therapy as an Adjuvant Treatment for Periodontitis: A Short-Term Evaluation of Inflammatory Periodontal Conditions and Bacterial Reduction in a Rat Model

Abstract: PDT used as an adjuvant treatment showed effective short-term control of periodontitis infection.

Cited by 46 publications

References 43 publications

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Effect of photodynamic therapy adjunct to scaling and root planing in periodontitis patients: A randomized clinical trial

Antimicrobial Photodynamic Therapy as a Strategy to Arrest Enamel Demineralization: A Short‐Term Study on Incipient Caries in a Rat Model^†

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Histomorphometric and Microbiological Assessment of Photodynamic Therapy as an Adjuvant Treatment for Periodontitis: A Short-Term Evaluation of Inflammatory Periodontal Conditions and Bacterial Reduction in a Rat Model

Abstract: PDT used as an adjuvant treatment showed effective short-term control of periodontitis infection.

Cited by 46 publications

References 43 publications

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Effect of photodynamic therapy adjunct to scaling and root planing in periodontitis patients: A randomized clinical trial

Antimicrobial Photodynamic Therapy as a Strategy to Arrest Enamel Demineralization: A Short‐Term Study on Incipient Caries in a Rat Model†

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Antimicrobial Photodynamic Therapy as a Strategy to Arrest Enamel Demineralization: A Short‐Term Study on Incipient Caries in a Rat Model^†