Histone Deacetylase 1 Down‐Regulation on Developmental Capability and Histone Acetylation in Bovine Oocytes and Parthenogenetic Embryos

Zhong, Wang; Zhao, Tiesuo; Zhang, P; Zhang, S; Guan, Jiyu; Ma, Xiaozhou; Yin, Yupeng; Zhang, J; Tang, Bo; Li, Z

doi:10.1111/j.1439-0531.2011.01778.x

Cited by 18 publications

(9 citation statements)

References 39 publications

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“…Oocyte collection and in vitro maturation were similar to previous research 50 . Briefly, bovine ovaries were collected from a local abattoir and transported to the laboratory within 4 h, then cumulus-oocyte complexes (COCs) were aspirated from the follicles using an 18-gauge needle attached to a 10 ml syringe.…”

Section: Methodssupporting

confidence: 63%

Aberrant DNA methylation reprogramming in bovine SCNT preimplantation embryos

Zhang

Chen

Wang

et al. 2016

Sci Rep

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DNA methylation reprogramming plays important roles in mammalian embryogenesis. Mammalian somatic cell nuclear transfer (SCNT) embryos with reprogramming defects fail to develop. Thus, we compared DNA methylation reprogramming in preimplantation embryos from bovine SCNT and in vitro fertilization (IVF) and analyzed the influence of vitamin C (VC) on the reprogramming of DNA methylation. The results showed that global DNA methylation followed a typical pattern of demethylation and remethylation in IVF preimplantation embryos; however, the global genome remained hypermethylated in SCNT preimplantation embryos. Compared with the IVF group, locus DNA methylation reprogramming showed three patterns in the SCNT group. First, some pluripotency genes (POU5F1 and NANOG) and repeated elements (satellite I and α-satellite) showed insufficient demethylation and hypermethylation in the SCNT group. Second, a differentially methylated region (DMR) of an imprint control region (ICR) in H19 exhibited excessive demethylation and hypomethylation. Third, some pluripotency genes (CDX2 and SOX2) were hypomethylated in both the IVF and SCNT groups. Additionally, VC improved the DNA methylation reprogramming of satellite I, α-satellite and H19 but not that of POU5F1 and NANOG in SCNT preimplantation embryos. These results indicate that DNA methylation reprogramming was aberrant and that VC influenced DNA methylation reprogramming in SCNT embryos in a locus-specific manner.

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Section: Methodssupporting

confidence: 63%

Aberrant DNA methylation reprogramming in bovine SCNT preimplantation embryos

Zhang

Chen

Wang

et al. 2016

Sci Rep

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“…It seems that high a level of histone acetylation was correlated with high developmental competence. However, knocking down histone deacetylase 1 (HDAC1) decreased the blastocyst formation rate of bovine parthenogenetic embryos despite the increase in acH3K14 [17]. The relationship between acetylation and developmental competence still needs to be further studied.…”

Section: Discussionmentioning

confidence: 99%

Valproic Acid Improves Porcine Parthenogenetic Embryo Development Through Transient Remodeling of Histone Modifiers

Huang

Yuan

et al. 2015

Cell Physiol Biochem

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Background/Aims: Parthenogenetic embryos are useful in many applications, such as being an alternative source of embryonic stem cells that would avoid ethical problems. Aberrance in epigenetic reprogramming is considered the major reason for the developmental failure of parthenogenetic embryos. Many histone deacetylase inhibitors have been shown to improve the reprogramming of stem cells and embryos. Here, the relationship between histone modification and parthenogenetic embryonic development was explored. Methods: Valproic acid (VPA) treatment was applied during the culture of parthenogenetic embryos. The abundance of histone modifiers was examined by immunofluorescence and quantified by Image-pro software. Results: The acH3K9 level in in vitro fertilized embryos was significantly higher than parthenogenetic embryos. VPA treatment improved both the blastocyst formation rate and the acH3K9 level in parthenogenetic embryos. The signal intensities of acH4K5 and H3K4me2 were also enhanced in VPA treated embryos. The H3K27me2 level was decreased in the VPA treated embryos at the 2-cell stage. However, the enhancement in the acH3K9, acH4K5 and H3K4me2 level, or the decrease in the H3K27me2 level disappeared shortly after VPA withdrawal. Conclusion: Optimizing histone modifications for a short time following activation was sufficient to enhance the in vitro development of parthenogenetic embryos.

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“…The mature oocytes were treated with 5 mM ionomycin for 5 min, followed by treatment with 2 mM 6-DMAP in synthetic oviduct fluid (Sofaa) [17] containing 10% FBS for 3.5 h at 38.5 C in 5% CO 2 and 95% humidified air. Treated oocytes were then washed in Sofaa three times and divided into two groups.…”

Section: Parthenogenetic Activation Of Bovine Oocytesmentioning

confidence: 99%

“…Then, COCs with intact, unexpanded cumulus cells were divided into two groups. One group of 20e25 COCs was cultured for 20e22 h at 38.5 C in 100 ml of maturation medium [17] in a humidified 5% CO 2 incubator. The second group was cultured in 100 ml of maturation medium supplemented with 200 mM RG108.…”

Section: In Vitro Maturation Of Bovine Oocytesmentioning

confidence: 99%