“…HepAD38 cells were cultured in a growth medium comprising Dulbecco’s modified Eagle’s medium (DMEM, HyClone, Logan, UT, USA) with the addition of 10% fetal bovine serum (FBS, Gibco, Rockville, MD, USA), 1 µg/mL tetracycline, 500 µg/mL G418, 100 units/mL penicillin (HyClone, Logan, UT, USA), and 100 µg/mL streptomycin (HyClone, Logan, UT, USA) as previously described. 12 To boost NTCP receptor expression in HepG2-NTCP, an additional 2 µg/mL of doxycycline was added to the growth medium. 13 …”