Histone modifications dictate specific biological readouts

Munshi, Anjana; Shafi, Gowhar; Aliya, Nishat; Jyothy, A.

doi:10.1016/s1673-8527(08)60094-6

Cited by 109 publications

(83 citation statements)

References 100 publications

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“…The resultant amino acid modifications are able to modulate histone function by affecting protein-protein or protein-DNA interactions. 40 Alongside our report that Nop58 K467 and K497 residues are SUMOylated, Matic et al published their findings that S502 in Nop58 is phosphorylated and is required for efficient SUMOylation of K497. 41 In addition, we have preliminary data suggesting that this region can also be ubiquitinated (Westman BJ, Lamond AI, data not shown).…”

Section: Nop58 and Sumo: What Are The Upstream Regulators?mentioning

confidence: 64%

A role for SUMOylation in snoRNP biogenesis revealed by quantitative proteomics

Westman

Lamond

2011

nucleus

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A role for SUMOylation in the biogenesis and/or function of Box C/D snoRNPs has been reported, mediated via SUMO2 conjugation to the core snoRNP protein, Nop58. A quantitative proteomics screen, based on SILAC (stable-isotope labeling by amino acids in cell culture) and mass spectrometry using extracts prepared from cultured mammalian cells expressing either 6His-SUMO1 or -SUMO2, revealed that the snoRNP-related proteins Nop58, Nhp2, DKC1 and NOLC1 are amongst the main SUMO-modified proteins in the nucleolus. SUMOylation of Nhp2 and endogenous Nop58 was confirmed using a combination of in vitro and cellbased assays and the modified lysines identified by site-directed mutagenesis. SUMOylation of Nop58 was found to be important for high-affinity Box C/D snoRNA binding and the localization of newly transcribed snoRNAs to the nucleolus. Here, these findings are reviewed and a model for understanding Nop58 SUMOylation in the context of Box C/D snoRNP biogenesis is presented. Given the essential role of snoRNPs in the modification of pre-ribosomal RNA, this work suggests that SUMO, snoRNPs and ribosome assembly, and thus cellular translation, growth and proliferation, may be linked via novel mechanisms which warrant further investigation.

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Section: Nop58 and Sumo: What Are The Upstream Regulators?mentioning

confidence: 64%

A role for SUMOylation in snoRNP biogenesis revealed by quantitative proteomics

Westman

Lamond

2011

nucleus

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“…150 While HP1α and HP1β are associated with silencing, other forms of HP1, such as HP1-type C, are found in euchromatin interacting with FACT subunit SSRP1, which is responsible for guiding FACT toward active genes to promote nucleosomal instability by H2AX exchange and RNAP activation. [103][104][105]151,152 Macromolecular Chromatin Euchromatin.…”

Section: Discussionmentioning

confidence: 99%

Basic concepts of epigenetics

Mazzio

Soliman²

2012

Epigenetics

203

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“…21,22 For example, acetylation of several lysine residues within the N-terminal tails of H3 and H4 gives rise to an open or "on" chromatin state typically associated with DNA hypomethylation and expressed genes, while de-acetylation tends to coincide with hypermethylated DNA loci that are not expressed. 23 Histone methylation can lead to different transcriptional consequences, depending on the residue affected. 22,24 In addition, epigenetic control can be mediated by means of recently discovered small regulatory RNAs.…”

Section: Ir-induced Microrna Expression In Mammary Gland Cellsmentioning

confidence: 99%