Histopathologic lesions in conventional pigs experimentally infected with Haemophilus parasuis serovar 5

Palzer, Andreas; Austin-Busse, Rose-Leáh; Ladinig, Andrea; Balka, Gyula; Zoels, Susanne; Ritzmann, M.

doi:10.15653/tpg-140905

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…We confirmed a strong inflammatory response characterized by high expression of inflammatory cytokines in skeletal muscle due to G. parasuis infection. This finding aligns with the well-known predilection sites of G. parasuis infection, such as the lung, arthrosis, pleura, peritoneum, kidney, and meninges [ 42 , 43 , 44 ]. It has been reported that G. parasuis breaks through the lung’s immune defense [ 45 ], allowing it to colonize various parts of the body, including skeletal muscle, through the bloodstream.…”

Section: Discussionsupporting

confidence: 87%

Transcriptome Analysis of miRNA and mRNA in Porcine Skeletal Muscle following Glaesserella parasuis Challenge

Zhou,

Chen,

Deng

et al. 2024

Genes

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Glaesserella parasuis (G. parasuis) causes systemic infection in pigs, but its effects on skeletal muscle and underlying mechanisms are poorly understood. We investigated G. parasuis infection in colostrum-deprived piglets, observing decreased daily weight gain and upregulation of inflammatory factors in skeletal muscle. Muscle fiber area and diameter were significantly reduced in the treated group (n = 3) compared to the control group (n = 3), accompanied by increased expression of FOXO1, FBXO32, TRIM63, CTSL, and BNIP3. Based on mRNA and microRNA (miRNA) sequencing, we identified 1642 differentially expressed (DE) mRNAs and 19 known DE miRNAs in skeletal muscle tissues between the two groups. We predicted target genes with opposite expression patterns to the 19 miRNAs and found significant enrichment and activation of the FoxO signaling pathway. We found that the upregulated core effectors FOXO1 and FOXO4 were targeted by downregulated ssc-miR-486, ssc-miR-370, ssc-miR-615, and ssc-miR-224. Further investigation showed that their downstream upregulated genes involved in protein degradation were also targeted by the downregulated ssc-miR-370, ssc-miR-615, ssc-miR-194a-5p, and ssc-miR-194b-5p. These findings suggest that G. parasuis infection causes skeletal muscle atrophy in piglets through accelerated protein degradation mediated by the “miRNAs-FOXO1/4” axis, while further research is necessary to validate the regulatory relationships. Our results provide new insights into the understanding of systemic inflammation growth mechanisms caused by G. parasuis and the role of miRNAs in bacterial infection pathogenesis.

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Section: Discussionsupporting

confidence: 87%

Transcriptome Analysis of miRNA and mRNA in Porcine Skeletal Muscle following Glaesserella parasuis Challenge

Zhou,

Chen,

Deng

et al. 2024

Genes

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Glässer's Disease

Aragón¹,

Segalés²,

Tucker³

2019

Diseases of Swine

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The use of porcine corrosion casts for teaching human anatomy

Eberlová

Liška

Mírka

et al. 2017

Annals of Anatomy - Anatomischer Anzeiger

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In teaching and learning human anatomy, anatomical autopsy and prosected specimens have always been indispensable. However, alternative methods must often be used to demonstrate particularly delicate structures. Corrosion casting of porcine organs with Biodur E20 Plus is valuable for teaching and learning both gross anatomy and, uniquely, the micromorphology of cardiovascular, respiratory, digestive, and urogenital systems. Assessments of casts with a stereomicroscope and/or scanning electron microscope as well as highlighting cast structures using color coding help students to better understand how the structures that they have observed as two-dimensional images actually exist in three dimensions, and students found using the casts to be highly effective in their learning. Reconstructions of cast hollow structures from (micro-)computed tomography scans and videos facilitate detailed analyses of branching patterns and spatial arrangements in cast structures, aid in the understanding of clinically relevant structures and provide innovative visual aids. The casting protocol and teaching manual we offer can be adjusted to different technical capabilities and might also be found useful for veterinary or other biological science classes.

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Histopathologic lesions in conventional pigs experimentally infected with Haemophilus parasuis serovar 5

Cited by 6 publications

References 13 publications

Transcriptome Analysis of miRNA and mRNA in Porcine Skeletal Muscle following Glaesserella parasuis Challenge

Transcriptome Analysis of miRNA and mRNA in Porcine Skeletal Muscle following Glaesserella parasuis Challenge

Glässer's Disease

The use of porcine corrosion casts for teaching human anatomy

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