Histopathologic-molecular Correlation in Early Mycosis Fungoides Using T-cell Receptor γ Gene Rearrangement by Polymerase Chain Reaction with Laser Capture Microdissection

Hsiao, Pa‐Fan; Hsiao, Cheng–Hsiang; Lin, Yu–Chen; Tseng, Mei-Ping; Tsai, Tsen‐Fang; Jee, Shiou‐Hwa

doi:10.1016/s0929-6646(09)60251-5

Cited by 19 publications

(9 citation statements)

References 27 publications

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“…The sensitivity of TRG amplification within a lesion is influenced by the frequency of the neoplastic clone in the population of infiltrating cells and by the complexity of the associated polyclonal repertoire. The neoplastic clone needs to occur at a frequency of about 5–10% for detection to occur 21,22 . Also, minor clonal populations are at greater risk of going undetected if they involve frequently rearranged Vγ and Jγ segments, which will be lost amidst the polyclonal repertoire 22 …”

Section: Discussionmentioning

confidence: 99%

Canine inflamed nonepitheliotropic cutaneous T‐cell lymphoma: a diagnostic conundrum

Moore

Affolter

Keller

2013

Veterinary Dermatology

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Section: Discussionmentioning

confidence: 99%

Canine inflamed nonepitheliotropic cutaneous T‐cell lymphoma: a diagnostic conundrum

Moore

Affolter

Keller

2013

Veterinary Dermatology

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“…The positive rate of TCR-γ gene rearrangement in early MF suspected cases was reported to be 40% to 71%[6],[7], which is useful for early diagnosis. Stevens et al [17] conducted logistic regression analysis on some clinical and laboratory data and established a simple clinical scoring system to facilitate MF diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic significance of TCR gene clonal rearrangement analysis in early mycosis fungoides

Wan²,

Wang³

et al. 2011

Chin J Cancer

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Mycosis fungoides (MF), the most common type of cutaneous T-cell lymphoma, has various unspecific clinical and histological characteristics. Its early diagnosis is challenging. The application of T-cell receptor (TCR) gene clonal rearrangement to the diagnosis of MF has been widely studied. In this study, we used polymerase chain reaction (PCR) to investigate the diagnostic significance of detecting TCR-γ and -β gene clonal rearrangement in the early diagnosis of mycosis fungoides. PCR for TCR-γ and TCR-β gene rearrangement was performed on 19 patients with suspected early MF, 6 with typical MF, and 6 with chronic dermatitis. Of the 19 patients with suspected early MF, 13 had TCR-γ gene clonal rearrangement, whereas none had TCR-β gene clonal rearrangement. All patients with typical MF had TCR gene clonal rearrangement, in which 4 showed TCR-γ clonal rearrangement, 1 showed TCR-β gene clonal rearrangements, and 1 showed both. No patients with chronic dermatitis had TCR gene clonal rearrangement. These results indicate that TCR gene clonal rearrangement analysis is a useful tool in diagnosing early MF. TCR-γ gene is recommended to the routine analysis, whereas TCR-β gene has potential in combination toward intractable cases.

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“…In this context, malignant cells tend to localize to the epidermis 11 -13 and in Pautrier collections. 14 Our case adds to the few reported instances in which microdissection has been used in the diagnosis of unusual or rare types of T-cell lymphoma 15 and is the first reported use of microdissection to identify a monoclonal proliferation in a case of suspected SPTCL. This technique may be useful to better characterize those patients with a clinical and histopathologic overlap between panniculitis and lymphoma.…”

Section: Discussionmentioning

confidence: 62%

“…Case series ranging from 5–29 cases have shown that microdissection can improve sensitivity of detection, particularly in early patch stage mycosis fungoides. In this context, malignant cells tend to localize to the epidermis 11–13 and in Pautrier collections 14 …”

Section: Discussionmentioning

confidence: 99%

Manual microdissection technique in a case of subcutaneous panniculitis‐like T‐cell lymphoma: a case report and review

et al. 2012

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Demonstration of T-cell receptor gene monoclonality often plays an important role in the diagnosis of T-cell lymphoma. When a test to detect monoclonality is performed on whole tissue sections, the presence of a reactive lymphocyte population may reduce sensitivity. This may be especially true for early or borderline cases of lymphoma. Microdissection techniques may be utilized to more readily identify a clonal population of lymphocytes. Subcutaneous panniculitis-like T-cell lymphoma represents a cutaneous lymphoid neoplasm whose clinical course may vary from an indolent, waxing and waning course to an aggressive course resulting in death. We report the first case of a microdissection technique used to facilitate diagnosing a case of subcutaneous panniculitis-like T-cell lympoma.

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Histopathologic-molecular Correlation in Early Mycosis Fungoides Using T-cell Receptor γ Gene Rearrangement by Polymerase Chain Reaction with Laser Capture Microdissection

Cited by 19 publications

References 27 publications

Canine inflamed nonepitheliotropic cutaneous T‐cell lymphoma: a diagnostic conundrum

Canine inflamed nonepitheliotropic cutaneous T‐cell lymphoma: a diagnostic conundrum

Diagnostic significance of TCR gene clonal rearrangement analysis in early mycosis fungoides

Manual microdissection technique in a case of subcutaneous panniculitis‐like T‐cell lymphoma: a case report and review

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