Histopathological and biomechanical evaluation of tenocyte seeded allografts on rat Achilles tendon regeneration

Güngörmüş, Cansın; Kolankaya, Dürdane; Aydin, Erkin

doi:10.1016/j.biomaterials.2015.01.077

Cited by 33 publications

(29 citation statements)

References 54 publications

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“…Human FDPT decellularized in 0.1% SDS and peracetic acid then seeded with adipose-derived MSCs implanted subcutaneously in nude mice remained healthy and viable for one month [ 86 ]. Reseeded Triton X-100-treated rat Achilles tendons were stronger and better organized after 24 hours in a surgical replacement model [ 87 ]. However, cell-laden tendon constructs cultured ex vivo without mechanical manipulation actively degrade their scaffolds, necessitating culture techniques that fulfill the need for cells to experience stimulation [ 88 ].…”

Section: Tendon Decellularizationmentioning

confidence: 99%

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

Youngstrom

Barrett

2015

Stem Cells International

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Tendons bridge muscle and bone, translating forces to the skeleton and increasing the safety and efficiency of locomotion. When tendons fail or degenerate, there are no effective pharmacological interventions. The lack of available options to treat damaged tendons has created a need to better understand and improve the repair process, particularly when suitable autologous donor tissue is unavailable for transplantation. Cells within tendon dynamically react to loading conditions and undergo phenotypic changes in response to mechanobiological stimuli. Tenocytes respond to ultrastructural topography and mechanical deformation via a complex set of behaviors involving force-sensitive membrane receptor activity, changes in cytoskeletal contractility, and transcriptional regulation. Effective ex vivo model systems are needed to emulate the native environment of a tissue and to translate cell-matrix forces with high fidelity. While early bioreactor designs have greatly expanded our knowledge of mechanotransduction, traditional scaffolds do not fully model the topography, composition, and mechanical properties of native tendon. Decellularized tendon is an ideal scaffold for cultivating replacement tissue and modeling tendon regeneration. Decellularized tendon scaffolds (DTS) possess high clinical relevance, faithfully translate forces to the cellular scale, and have bulk material properties that match natural tissue. This review summarizes progress in tendon tissue engineering, with a focus on DTS and bioreactor systems.

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Section: Tendon Decellularizationmentioning

confidence: 99%

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

Youngstrom

Barrett

2015

Stem Cells International

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“…Moreover, We found that immunostaining positive signals of type I collagen was increased in defects receiving both DTS and CS compared with those receiving DTS alone, and the intensity of immunostaining signal was greater in DTS group compared with the control group. These variations in histomorphometry and immunohistochemistry may account for the biomechanical property difference among each groups at 6 and 12 weeks postoperatively, as tenocyte density, collagen alignment and type were reported to be relevant to the biomechanical strength of the tendon . Potential explanations for this improvement may be the biomaterial cues on tenogenic differentiation and paracrine secretion of stem cells, which promote collagen deposition and protein synthesis, as well as the regeneration of tenocytes, resulting in an improvement of mechanical properties such as failure load and ultimate strength.…”

Section: Discussionmentioning

confidence: 99%

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Xie

Zhou

Tang

et al. 2019

Journal Orthopaedic Research

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Tissue‐engineering approaches have great potential to improve the treatment of tendon injuries which are major musculoskeletal disorders. The purpose of this study was to assess the tissue engineering potential of a novel multilayered decellularized tendon “book” scaffold with bone marrow mesenchymal stem cells (BMSCs) sheets for repair of an Achilles tendon defect in a rabbit model. In this study, we developed a novel book‐shaped decellularized scaffold derived from the extracellular matrix of tendon tissues from New Zealand white rabbits. Hematoxylin and eosin (H&E) staining, 4′, 6‐diamidino‐2‐phenylindole (DAPI) staining, DNA quantitation, and scanning electron microscopy (SEM) confirmed the efficiency of decellularization. After culturing BMSCs on decellularized scaffolds, 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT) assay, SEM, quantitative real time polymerase chain reaction (qRT‐PCR), and immunofluorescence analysis demonstrated that decellularized scaffolds have the capacity to yield homogeneous distribution and alignment of BMSCs, as well as support their differentiation into tendon. Tenomodulin and Alpha‐1 collagen type I are important indicators for evaluating tenogenic differentiation of BMSCs. When decellularized “book” scaffolds with BMSCs sheets were used to repair a 1 mm Achilles tendon defect, histomorphological analysis, immunohistochemical assessment, and biomechanical testing showed that the book‐shaped decellularized tendon matrix scaffold and BMSCs sheets could promote the regeneration of type I collagen at the wound site during healing, and improve the mechanical properties of the repaired tendon. Therefore, the results of this study suggest that the novel decellularized “book” tendon scaffolds combined with BMSCs sheets have therapeutic effects on improving the healing quality of the Achilles tendon. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–11, 2019.

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“…Despite this, the use of ECM-derived scaffolds have been translated into the tendon arena and are one of the most commonly used methods for augmenting tendon repair [62,63]. Again the promising results reported in animal models [64], have not been borne out in human trials. While observational studies have concluded in favour of porcine small intestinal submucosa (SIS) grafts [65,66].…”

Section: Decellularized Extracellular Matrixmentioning

confidence: 99%

Augmenting endogenous repair of soft tissues with nanofibre scaffolds

Baldwin

Snelling

Dakin

et al. 2018

J. R. Soc. Interface.

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As our ability to engineer nanoscale materials has developed we can now influence endogenous cellular processes with increasing precision. Consequently, the use of biomaterials to induce and guide the repair and regeneration of tissues is a rapidly developing area. This review focuses on soft tissue engineering, it will discuss the types of biomaterial scaffolds available before exploring physical, chemical and biological modifications to synthetic scaffolds. We will consider how these properties, in combination, can provide a precise design process, with the potential to meet the requirements of the injured and diseased soft tissue niche. Finally, we frame our discussions within clinical trial design and the regulatory framework, the consideration of which is fundamental to the successful translation of new biomaterials.

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Histopathological and biomechanical evaluation of tenocyte seeded allografts on rat Achilles tendon regeneration

Cited by 33 publications

References 54 publications

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Augmenting endogenous repair of soft tissues with nanofibre scaffolds

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Histopathological and biomechanical evaluation of tenocyte seeded allografts on rat Achilles tendon regeneration

Cited by 33 publications

References 54 publications

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration

­Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Augmenting endogenous repair of soft tissues with nanofibre scaffolds

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Product

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Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit