Histopathological and immunohistochemical studies of membranes of deacetylated chitin derivatives implanted over rat calvaria

Hidaka, Yuichi; Ito, Michio; Mori, Koichi; Yagasaki, Hiroshi; Kafrawy, Abdel H.

doi:10.1002/(sici)1097-4636(19990905)46:3<418::aid-jbm15>3.0.co;2-t

Cited by 73 publications

(47 citation statements)

References 14 publications

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“…Previous studies have shown that chitosan demonstrates chemotactic potential in vitro and in vivo . 25–27 There are many studies, which showed minimal inflammatory response of chitosan, 28–29 but there also exists many studies which show that chitosan could lead to high degree of inflammatory infiltrates 30–31 and that reaction could last for long periods. 32 But in our case, for chitosan-TPP and chitosan-TPP-BMP-7 microparticles fabricated by coacervation technique, we observed minor inflammatory response at week 6, which reduced by 12 weeks period.…”

Section: Discussionmentioning

confidence: 99%

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Mantripragada

Jayasuriya

2016

Materials Science and Engineering: C

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Injectable chitosan microparticles were prepared using a simple coacervation method under physiologically friendly conditions by eliminating oil or toxic chemical, and employing low temperature and pressure for growth factor stability. Amount of 200 ng of bone morphogenetic protein-7 (BMP-7) was incorporated in the chitosan microparticles by two methods: encapsulating and coating techniques. These microparticles were tested in vivo to determine the biological response in a rat femoral bone defect at 6 and 12 weeks. Four groups (n=10) were tested which include two groups for BMP-7 incorporated microparticles (by two techniques), microparticles without BMP-7, and defect itself (negative control). Healthy bone formation was observed around the microparticles, which were only confined to the defect site and did not disperse. Histology indicated minor inflammatory response around the microparticles at 6 weeks, which reduced by 12 weeks. Micro-CT analysis of bone surface density and porosity was found to be significantly more (p<0.05) for microparticles containing groups, in comparison with controls, which suggests that the new bone formed in the presence of microparticles is more interconnected and porous. Collagen fibrils analysis conducted using multiphoton microscopy showed significant improvement in the formation of bundled collagen area (%) in microparticles containing groups in comparison with controls, indicating higher cross-linking between the fibrils. Microparticles were biocompatibe and did not degrade in the 12 week implant period.

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Section: Discussionmentioning

confidence: 99%

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Mantripragada

Jayasuriya

2016

Materials Science and Engineering: C

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“…Chitosan degree of deacetylation (DDA) has often been cited as an important parameter that determines many physiochemical and biological properties of chitosans such as crystallinity, hydrophilicity, degradataion and cell response [6,7,8,9,10,11]. For example, Freier et al .…”

Section: Introductionmentioning

confidence: 99%

“…Hidaka et al . [10] reported that chitosan membranes between 65 and 80% DDA elicited marked inflammatory reactions that subsided in time with degradation of the films, granulation tissue formation, and osteogensis while membranes made of 94% DDA chitosan showed minimal degradation, mild inflammation and minimal osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

Deacetylation of Chitosan: Material Characterization and in vitro Evaluation via Albumin Adsorption and Pre-Osteoblastic Cell Cultures

et al. 2011

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Degree of deacetylation (DDA) and molecular weight (MW) of chitosans are important to their physical and biological properties. In this study, two chitosans, HS (DDA = 73.3%) and AT (DDA = 76.8%), were deacetylated with 45% sodium hydroxide under nitrogen atmosphere at 80 °C or 90 °C for up to 120 min, to obtain two series of chitosans. The polymers produced were characterized for MW by gel permeation chromatography, DDA by titration and UV-vis methods, and crystallinity, hydrophilicity and thermal stability by X-ray diffraction, water contact angle and differential scanning calorimetry respectively. Films, made by solution casting in dilute acetic acid at ambient conditions, were evaluated for biological activity by albumin adsorption and the attachment and growth of a pre-osteoblast cell line. Chitosans with between 80–93% DDA’s (based on titration) were reproducibly obtained. Even though deacetylation under nitrogen was supposed to limit chain degradation during decetylation, MW decreased (by maximum of 37.4% of HS and 63.0% for AT) with increasing deacetylation reaction time and temperature. Crystallinity and decomposition temperature increased and water contact angles decreased with processing to increase DDA. Significantly less albumin was absorbed on films made with 93% DDA chitosans as compared with the original materials and the AT chitosans absorbed less than the HS chitosans. The cells on higher DDA chitosan films grew faster than those on lower DDA films. In conclusion, processing conditions increased DDA and influenced physicochemical and biological properties. However, additional studies are needed to unambiguously determine the influence of DDA or MW on in vitro and in vivo performance of chitosan materials for bone/implant applications.

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“…Hidaka [18] reported that a 94% DDA chitosan membranes gave minimal film degradation, mild inflammatory reaction and minimal osteogenesis while between 65 to 80% gave the opposite response.This effect is more or less in line to our survival rate results for all three different cell lines ( Figure) that the higher the degree of de-acetylation the more the survival rate and that 87% de-acetylation actually improved the cell survival rate to above 100%.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Different Degrees of De-Acetylated Chitosan on 3T3- and Two Human Tooth Fibroblast Cell-Lines

SR¹,

Olivier²,

HW³

et al. 2016

IJDOS

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Histopathological and immunohistochemical studies of membranes of deacetylated chitin derivatives implanted over rat calvaria

Cited by 73 publications

References 14 publications

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Deacetylation of Chitosan: Material Characterization and in vitro Evaluation via Albumin Adsorption and Pre-Osteoblastic Cell Cultures

Cytotoxicity of Different Degrees of De-Acetylated Chitosan on 3T3- and Two Human Tooth Fibroblast Cell-Lines

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