Histopathological effects and immunolocalization of periplocoside NW from Periploca sepium Bunge on the midgut epithelium of Mythimna separata Walker larvae

Feng, Mingxing; Shi, Baojun; Zhao, Yanchao; Hu, Zhaonong; Wu, Wenjun

doi:10.1016/j.pestbp.2014.09.001

Cited by 22 publications

(17 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our preliminary results revealed that the BBMVs from the midgut epithelium cells of M. separata larvae possess the initial action site of periplocosides [ 11 ]. Therefore, the BBMVs of M. separata larvae was isolated and subjected to affinity chromatography to separate the binding proteins of periplocosides.…”

Section: Resultsmentioning

confidence: 99%

“…Ultrastructural observations showed that microvilli, organelle, and cytomembrane are destructed in the midgut cells of M. separata larvae [ 10 ]. Research on histopathological effects indicated that brush-border membrane vesicles (BBMVs) from the midgut epithelium cells of M. separata larvae are the initial action site; one or more binding sites may also exist in the larvae [ 11 ]. However, the specific binding sites and insecticidal mechanism of periplocosides in M. separata larval midgut cells remain unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Feng

He²,

Wang

et al. 2016

Toxins

Self Cite

View full text Add to dashboard Cite

Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Feng

He²,

Wang

et al. 2016

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our research group has focused on the isolation and identification of systemically active compounds from P. sepium [ 10 , 11 , 12 ]. Periplocoside P (PSP), a novel compound isolated from P. sepium has high insecticidal activity (LC 50 = 110 mg·L −1 ) against Mythimna separata [ 13 ]. To determine the specific mechanism of action of periplocosides against M. separata larvae, we performed systematic research and showed that putative targets of periplocosides may exist in the brush border membrane vesicles (BBMVs) of the larvae midgut [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis of the Effect of Periplocoside P from Periploca sepium on Brush Border Membrane Vesicles in Midgut Epithelium of Mythimna separata Larvae

Feng

Wei

et al. 2017

Toxins

Self Cite

View full text Add to dashboard Cite

Periplocoside P (PSP), a novel compound isolated from Periploca sepium Bunge, possesses insecticidal activity against some lepidopterans, such as Mythimna separata. In M. separata, the brush border membrane vesicles of the midgut epithelium are the initial site of action of periplocosides. We conducted two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry analysis to analyze differentially expressed proteins (DEPs) from periplocoside P (PSP)-treated M. separata. We successfully isolated seven up-regulated and three down-regulated DEPs that have been previously identified, as well as a novel DEP. The DEPs are implicated in protein degradation, transporter, folding, and synthesis, and in juvenile hormone biosynthesis. DEPs involved in the oxidative phosphorylation energy metabolism pathway are enriched. Through real-time polymerase chain reaction assay, we confirmed that vma1 expression is significantly up-regulated expression levels in PSP-treated M. separata larvae. Enzymology validation further indicated that PSP can significantly inhibit V-type ATPase activity in a concentration-dependent manner. Given these results, we speculate that in M. separata, the V-type ATPase A subunit in the midgut epithelium is the putative target binding site of periplocosides. This finding provides preliminary evidence for the mode of action of periplocosides.

show abstract

“…Preliminary histopathological and ultrastructural studies showed that periplocosides disrupt the midgut epithelial cells of lepidopteran larvae1920. According to the results from fluorescence localization and immunolocalization studies of PSNW, the main mechanism of toxicity is by damage to the midgut cell and organellar membranes in M. separata larvae; the deduced binding site is the cell membrane of the midgut epithelial cells2021. Alternatively, the results of protease activity-determination demonstrated that PSP can activate the midgut proteases of M. separata , particularly the trypsin-like protease22.…”

mentioning

confidence: 99%

Effects of Periplocoside P from Periploca sepium on the Midgut Transmembrane Potential of Mythimna separata Larvae

Wang

Zhāng

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Periplocoside P (PSP) isolated from the root bark of Periploca sepium contains a pregnane glycoside skeleton and possesses high insecticidal properties. Preliminary studies indicated that PSP disrupts epithelial functions in the midgut of lepidopteran larvae. In the present study, we examined the effects of PSP on the apical and basolateral membrane voltages, Va and Vbl, respectively, of cells from (1) midguts isolated from the larvae of the oriental armyworm Mythimna separata that were in vitro incubated with toxins and (2) midguts isolated from M. separata larvae force-fed with PSP. We compared the effects of PSP with the effects of the Bacillus thuringiensis toxin Cry1Ab and inactive periplocoside E (PSE) on the midgut epithelial cells. The results showed that Va rapidly decreased in the presence of PSP in a time- and dose-dependent manner, similar to the effects of Cry1Ab. By contrast, PSE did not affect the Va and Vbl. Additionally, PSP did not influence the Vbl. Given these results, we speculate that PSP may modulate transport mechanisms at the apical membrane of the midgut epithelial cells by inhibiting the V-type H+ ATPase.

show abstract

Histopathological effects and immunolocalization of periplocoside NW from Periploca sepium Bunge on the midgut epithelium of Mythimna separata Walker larvae

Cited by 22 publications

References 21 publications

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Comparative Proteomic Analysis of the Effect of Periplocoside P from Periploca sepium on Brush Border Membrane Vesicles in Midgut Epithelium of Mythimna separata Larvae

Effects of Periplocoside P from Periploca sepium on the Midgut Transmembrane Potential of Mythimna separata Larvae

Contact Info

Product

Resources

About