Histopathological features of low-dose organophosphate exposure

Cobilinschi, Cristian; Tincu, R.C.; Cobilinschi, Claudia; Neagu, Tiberiu Paul; Becheanu, Gabriel; Sinescu, Ruxandra Diana; Checheriţă, Ionel Alexandru; Grințescu, Ioana Marina; Lascăr, Ioan

doi:10.47162/rjme.61.2.11

Cited by 8 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of inflammation in organophosphates, particularly CPS-induced renal injury, has been previously reported [ 7 , 52 ]. This aligns with the observed oxidative stress in CPS-intoxicated rats in this study.…”

Section: Discussionmentioning

confidence: 99%

Berberine alleviates chlorpyrifos-induced nephrotoxicity in rats via modulation of Nrf2/HO-1 axis

Binmahfouz,

Hassanein,

Bagher

et al. 2024

Heliyon

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Berberine alleviates chlorpyrifos-induced nephrotoxicity in rats via modulation of Nrf2/HO-1 axis

Binmahfouz,

Hassanein,

Bagher

et al. 2024

Heliyon

View full text Add to dashboard Cite

“…who administered 100 mg/Kg BW of chlorpyrifos show that the kidney has dilatation and atrophy of the tubules from histopathological observation. [ 23 ]…”

Section: Discussionmentioning

confidence: 99%

The effect of chlorpyrifos oral exposure on the histomorphometric and kidney function in Wistar rat

Sakinah,

Wisudanti,

Abrori

et al. 2024

Indian Journal of Pharmacology

View full text Add to dashboard Cite

BACKGROUND: Chlorpyrifos belongs to a broad-spectrum organophosphate insecticide that has high toxicity, is metabolized in the liver by the oxidation reaction, and can inhibit acetylcholinesterase activity. Acetylcholinesterase inhibition generates the reactive oxygen species and induces oxidative stress, which ultimately results in cellular damage like in the kidney. Examining blood urea nitrogen (BUN) levels, creatinine, and kidney histopathology is an appropriate indicator to assess the toxicity of chlorpyrifos to the degree of damage to cells and kidney tissue. MATERIALS AND METHODS: This research used to determine the effect of duration of exposure to chlorpyrifos and dose–response relationships is important for early detection of the effects of chlorpyrifos toxicity on health. The research study was a true experimental (completely randomized design) consisting of 30 subjects divided into 5 groups. Controlled Group (K1) given 1 mg/kg BW Tween 20 and NaCl 0, 9% until the 56th day. The chlorpyrifos exposed group (P1, P2, P3, and P4) was given chlorpyrifos 5 mg/kg BW for 7, 14, 28, and 56 days. After the treatment, BUN and creatinine levels were measured, and microscopic changes in the kidney were analyzed. The results of BUN, creatinine, and kidney histopathologic were analyzed using the analysis of variance statistical test. RESULTS: The data result showed that compared to the control group, there were significant increases of BUN and creatinine (P = 0.013 and P = 0.003). Histopathological examinations of kidney glomerulus diameter were also smaller compared to the control group (P = 0.00). All the data measurement indicates significant differences compared to the control group. CONCLUSIONS: We concluded that sub-chronic oral exposure to chlorpyrifos at low doses can damage the kidneys and cause kidney failure.

show abstract

“…However, the range of cholinergic signs can be diverse with different inhibitors, the same inhibitor in different classes, or the same inhibitor with different ways of exposure (5). The general dysfunction seems due to inhibition, revealing the primary toxic effect on the endocrine system but it can also affect other organs such as the liver and kidneys (1). Chlorpyrifos is categorized as a moderately toxic pesticide in rats and mice with a lethal toxic dose (LD50) between 50-500 mg/kg body weight.…”

Section: Introductionmentioning

confidence: 99%

Histological Changes Induced by Organophosphate Chlorpyrifos in Liver Tissue of Adult Male Rabbits

EL Daffri,

Mohammed,

Amraje

et al. 2022

sjuob

View full text Add to dashboard Cite

Background: Chlorpyrifos (CPF) is one of the most common pesticides that is extensively applied in agriculture and animal houses. Several experimental studies on rats demonstrated that exposure to CPF elicits a group of deleterious effects including hepatic dysfunction, immunological defect and neurotoxicity. Moreover, there is concern regarding their deleterious effects on human life. This study investigated the influence of chlorpyrifos on the hepatic tissue of adult male rabbits in long-term exposure and evaluated the protective role of vitamin E in ameliorating toxic impacts. Methods: Twenty adult male rabbits were used in the study. Their average weight was (27.6 ±1.891) Kg. They were divided into four groups, five animals in each. The control group received only distilled water. The CPF-treated group was administrated 33.3mg/Kg of insecticide orally by gastric gavage. The vitamin E group received only Vit E; 150mg/kg body weight. Animals in the combination group were treated with CPF and given Vit E at the same time. The treatment was applied once daily in the morning after a food supplement for 12 weeks. Animals were then anesthetized with chloroform, their livers were removed, placed in formalin, processed and stained with hematoxylin and eosin, and with Masson's trichrome Results: Histopathological changes showed that CPF caused degenerative changes in hepatocytes in terms of vacuolization of cytoplasm, congestion, dilatation of the sinusoidal lumen, and leukocytic infiltration of liver parenchyma, as well as the presence of signs of fibrosis manifested by trichrome stain indicating the chronic effect of pesticides. In the combination group, the majority of hepatocytes presented with normal structures, and the amount of fibrosis was diminished around the portal area. Additionally, congestion appeared to a milder degree than in the experimental group, suggesting the antioxidant role of vitamin E in reducing toxic effects. Conclusion: The study concluded that CPF could cause hepatocellular damage to the rabbits' tissue which can be alleviated by applying antioxidants such as vitamin E.

show abstract

Histopathological features of low-dose organophosphate exposure

Cited by 8 publications

References 37 publications

Berberine alleviates chlorpyrifos-induced nephrotoxicity in rats via modulation of Nrf2/HO-1 axis

Berberine alleviates chlorpyrifos-induced nephrotoxicity in rats via modulation of Nrf2/HO-1 axis

The effect of chlorpyrifos oral exposure on the histomorphometric and kidney function in Wistar rat

Histological Changes Induced by Organophosphate Chlorpyrifos in Liver Tissue of Adult Male Rabbits

Contact Info

Product

Resources

About