Histopathological Study of Infection Process of Colletotrichum gloeosporioides Penz and Sacc. on Mangifera indica L.

Pandey, Ashutosh Kumar; Pandey, Brijesh; Muthukumar, M.; Yadava, L.P.; Chauhan, U.K.

doi:10.3923/ppj.2012.18.24

Cited by 12 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SIX genes have been identified in five non-Fusarium species. Two of these species are known to invade host xylem cells during infection (E. turcicum and V. nonalfalfae) similar to F. oxysporum, while the other three species (Ustilaginoidea virens, C. orbiculare, and C. gloeosporioides) do not colonize the xylem (Pandey et al, 2012;Li et al, 2013;Yadeta and Thomma, 2013;Marton et al, 2018;Kotze et al, 2019). Although some of the SIX effectors are required for pathogenicity, lead to host responses upon recognition, and can interact with R-genes, the biological function of the majority of these genes is unknown (Ma et al, 2015;Niu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Time-Course RNAseq Reveals Exserohilum turcicum Effectors and Pathogenicity Determinants

2020

View full text Add to dashboard Cite

Exserohilum turcicum (sexual stage Setosphaeria turcica) is the hemibiotrophic causal agent of northern leaf blight of maize and sorghum. This study aimed to identify the genes involved in host colonization during the biotrophic and necrotrophic phases of infection. It also aimed to identify race-specific differences in gene expression. RNAseq of maize seedlings inoculated with a race 13N or 23N E. turcicum isolate was conducted before inoculation and at 2, 5, 7, and 13 days post-inoculation (dpi). Biological replicates were pooled per time point for each race and sequenced. A bioinformatics pipeline was used to identify candidate effectors, and expression was validated for selected candidates. Fungal biomass was positively correlated with the percentages of E. turcicum reads mapped, which were low at early time points (2-7 dpi) with a significant increase at 13 dpi, indicating a lifestyle switch from biotrophy to necrotrophy between 7 and 13 dpi. AVRHt1 is the putative E. turcicum effector recognized by the maize resistance gene Ht1. Consistent with this, AVRHt1 was expressed in planta by race 23N, but transcripts were absent in race 13N. In addition, specific transposable elements were expressed in 23N only. Genes encoding the virulence-associated peptidases leupeptin-inhibiting protein 1 and fungalysin were expressed in planta. Transcriptional profiles of genes involved in secondary metabolite synthesis or cell wall degradation revealed the importance of these genes during late stages of infection (13 dpi). A total of 346 expressed candidate effectors were identified, including Ecp6 and proteins similar to the secreted in xylem (SIX) effectors common to formae speciales of Fusarium oxysporum, SIX13 and SIX5. Expression profiling of Ecp6 and SIX13-like indicated a peak in expression at 5 and 7 dpi compared to 2 and 13 dpi. Sequencing of SIX13-like from diverse isolates of E. turcicum revealed host-specific polymorphisms that were mostly non-synonymous, resulting in two groups of SIX13-like proteins that corresponded to the maize or sorghum origin of each isolate. This study suggests putative mechanisms whereby E. turcicum causes disease. Identification of the candidate effector SIX13-like is consistent with the infection mode of E. turcicum through the xylem of susceptible hosts.

show abstract

Section: Discussionmentioning

confidence: 99%

Time-Course RNAseq Reveals Exserohilum turcicum Effectors and Pathogenicity Determinants

2020

View full text Add to dashboard Cite

show abstract

“…The dehydration of samples were processed using series of chemicals (alcohol 30, 50, 70, 80 and 95%; absolute alcohol, alcohol 75% + xylene 25%; alcohol 50% + xylene 50%; alcohol 25% + xylene 75% and pure xylene for 30 min each respectively) using automatic tissue processor (Yorko, New Delhi) and embedded in melted paraffin wax (54 to 56°C) for at least 4-8 h in order to completely replace the xylene with paraffin wax Pandey et al, 2012).…”

Section: Dehydration and Embedding Of Samplementioning

confidence: 99%

HOST PATHOGEN INTERACTION STUDY IN MALFORMED AFFECTED TISSUES OF <i>MANGIFERA INDICA</i> L.

Kumar¹,

Kamle²,

Gupta³

et al. 2013

American Journal of Agricultural and Biological Sciences

View full text Add to dashboard Cite

The malformation disease of mango by Fusarium moniliforme var. subglutinan is one of the major causes of huge economical loss every year. Early diagnosis of the pathogens can help cultivators to protect the mango orchid and fruits from successive and secondary infection. The molecular detection is one of the better choices among available techniques for diagnosis. But the farmers or individual cultivars from tropical and subtropical countries like India, Pakistan, Malaysia, Vietnam, Philippines, have lots of limitation to use molecular method. Another problem with this pathogen is lack of specific biomarker. Thus the present study was designed to develop a morphological and histopathological methodology to detect Fusarium moniliforme var. subglutinans and its host-pathogen interaction between fungus and hot tissues. Fresh and preserved (Formaldehyde: Acetic Acid: Alcohol (FAA) solution) tissues were used to check the efficacy of the method. The inter and intracellular distribution of fungal hyphae, in various tissue like cortex, phloem and parenchymatous pith cell, was clearly revealed by this method. The characteristics which were observed like detection of the mycelium threads at the juncture of the shoot tip, axillary buds, petels and sepals axis of the malformed or infected tissues. The Resin accumulation in the parenchymatous and other cells of vegetative tissue was also found as characteristics of this pathogen infection. This study is being also important in terms of identification and growth behavior of the pathogen.

show abstract

“…Other than that, existence or ability of the fungus to infect other hosts which are closely related to S. trifasciata has yet to be discovered. Moreover, infection processes of Colletotrichum species such as C. gloeosporioides and C. acutatum towards the hosts have been extensively studied (Curry et al 2002; Talhinhas et al 2011; Pandey et al 2012; Marques et al 2013). In spite of that, histopathological study of infection of C. sansevieriae is relatively limited.…”

Section: Introductionmentioning

confidence: 99%

Identification, pathogenicity and histopathology of Colletotrichum sansevieriae causing anthracnose of Sansevieria trifasciata in Malaysia

2020

View full text Add to dashboard Cite

Aims: To characterize causal pathogen of Sansevieria trifasciata anthracnose through morphology and molecular analysis; to evaluate the host range of the pathogen; and to explicate the infection process by the pathogen histopathologically. Methods and Results: Symptomatic leaves of S. trifasciata were collected from five states in Malaysia. The causal pathogen was isolated and identified for the first time in Malaysia as C. sansevieriae based on morphological and multigene phylogenetic analyses using ITS, TUB2 and GAPDH sequences. Pathogenicity tests were conducted on different hosts. Colletotrichum sansevieriae was not pathogenic towards S. cylindrica, S. masoniana, Furcraea foetida, Chlorophytum comosum, Aloe vera and Gasteria carinata, confirming the exceptionally high host specificity for a species of Colletotrichum. Histopathology was performed using light microscope and scanning electron microscopy to study the infection process of C. sansevieriae on S. trifasciata. Colonization of host leaves by the pathogen was observed 2 days after inoculation. Conclusions: Colletotrichum sansevieriae caused anthracnose of S. trifasciata in Malaysia. It is a host-specific pathogen and colonized the host intracellularly. Significance and Impact of the Study: This is the first report of C. sansevieriae causing anthracnose of S. trifasciata in Malaysia. The host range test and understanding of the infection process will provide better understanding of the host-pathogen relationship and beneficial for effective disease management.

show abstract

Histopathological Study of Infection Process of Colletotrichum gloeosporioides Penz and Sacc. on Mangifera indica L.

Cited by 12 publications

References 18 publications

Time-Course RNAseq Reveals Exserohilum turcicum Effectors and Pathogenicity Determinants

Time-Course RNAseq Reveals Exserohilum turcicum Effectors and Pathogenicity Determinants

HOST PATHOGEN INTERACTION STUDY IN MALFORMED AFFECTED TISSUES OF <i>MANGIFERA INDICA</i> L.

Identification, pathogenicity and histopathology of Colletotrichum sansevieriae causing anthracnose of Sansevieria trifasciata in Malaysia

Contact Info

Product

Resources

About