Histopathological study of invasive and non-invasive Entamoeba spp. in experimental rats

Abstract

Background: The genus Entamoeba has many species that are invasive or non-invasive (E. histolytica, E. dispar, and E. moshkovskii).The invasive E. histolytica is the main pathogenic amoeba in human. Amoebiasis involves several stages starting with the adherence of the parasite to the intestinal epithelium, followed by degradation, tissue invasion, and distribution to other organs. Results: The current study investigates the pathological changes of Entamoeba spp. infection in both rectum and cecum of experimental rats. The results showed the histological changes at the 7th, 14th, and 28th day post-infection for the three species. E. histolytica and E. moshkovskii infection showed less pathological changes compared to E. histolytica. These changes include the attachment of the trophozoites to the mucosal layer, significant surface epithelial changes such as dissociation and degeneration in the mucosal layer, and ulceration of the apical surface. Inflammatory cells infiltrate the varied regions, extending into the deep mucosa causing mild architectural alterations. These are features of amoebiasis. Conclusion: The pathological changes reported in E. dispar and E. moshkovskii were less severe than E. histolytica.

Serological detection for Entamoeba histolytica among cancer patients suffering from diarrhea in Thi-Qar province/ Southern Iraq

“…The age of cancer patients who has parasitic infection with E. histolytica that were also checked based on the requirements of the current study and the statistical analysis showed a significant difference as explained in table (4), where the high rate of parasitic infection was reported among cancer patients with (51-60) age group while the low rate of parasitic infection was reported among (21-30) age groups of cancer patients . χ 2 = 135.58 df = 6 P-value = 0.00 S: significant differences P≤ 0.05…”

“…The invasive E. histolytica is the main human zoonotic amoeba. The parasite must stick to the intestinal epithelium, which is caused degradation, then penetrate the tissue and spread to other organs (4). Children who live in developing countries, diarrhea is the biggest killer, accounting for 9% of all deaths in children under the age of five (5).…”