Histopathological Study of Matrix Mineralization by Osteoblastic-like and Odontoblastic-like Cells in Diffusion Chamber

Rodrı́guez, Augusto; Borkosky, Silvia Susana; Han, Phuu Pwint; Tsujigiwa, Hidetsugu; Takagi, Tohru; Ara, Sathi Gulsan; Lee, You-Jin; Katase, Naoki; Okauchi, Mika; Nakano, Keisuke; Xiao, Jing

doi:10.2485/jhtb.15.6

Cited by 2 publications

(4 citation statements)

References 10 publications

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“…It is noteworthy that the oxygen concentration could play a crucial role for chondro-osteogenic cell differentiation 13) . Osteoblastic differentiation in the presence of excessive vessel formation as in this case, is a feature of membranous ossification 4,5) .…”

Section: Discussionmentioning

confidence: 94%

“…Moreover, we have also published that their mechanism of bone induction is similar to intramembranous ossification 4,5) . Until now, the origin of the ectopic bone has not been reported yet.…”

Section: Introductionmentioning

confidence: 88%

“…KUSA/A1 cell is a marrow stromal cell line from primary bone marrow culture of female C3H/He mouse 1) . KUSA/A1 cells are capable to differentiate into osteoblasts and induce mineralized bone matrix 2) . We have reported that a large amount of new bone can produce using KUSA/A1 cells with honeycomb scaffold 3) .…”

Section: Introductionmentioning

confidence: 99%

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Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier

Rodrı́guez

Nagatsuka

Tsujigiwa

et al. 2007

J. Hard Tissue Biology.

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The basic principle of bone tissue engineering is to use seeded stem cells in porous scaffold. Stem cells can proliferate and differentiate into various types of mature cells. A kind of stem cell called KUSA/A1 is a marrow stromal cell, capable of differentiating into three mesenchymal phenotypes: osteocyte, adipocyte, and myocyte by treating with 5-azacytidine in cell culture. Moreover, it has been reported that the mechanism of bone induction by KUSA/A1 cells is similar to intramembranous ossification.In order to clarify the origin of osteoblasts implicated in new bone formation, KUSA/A1 cells alone and combined with Honeycomb carrier were implanted in Transgenic Green Fluorescent Protein mice (GFP) mice. The presence of GFP positive host cells with osteoblastic morphology as well as GFP negative cells, clearly of KUSA/A1 cells in origin were observed around the bony trabeculae. These results indicated that the new bone was not only produced by KUSA/A1 cells but also by host cells from the surrounding connective tissues.To our knowledge, this is the first study to describe that host cells play an important role in ectopic bone induced by implanted marrow stromal cells, which would need special attention in bone tissue engineering.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 88%

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Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier

Rodrı́guez

Nagatsuka

Tsujigiwa

et al. 2007

J. Hard Tissue Biology.

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“…Various studies have investigated the dental pulp–derived cell for characterization and identification of specific marker of these cells . However, to our knowledge, there is not yet a report of a long‐term stably established cells, which duplicate osteoblastic nature and stem cell–like character both in vitro and in vivo .…”

Section: Introductionmentioning

confidence: 99%

Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

Tsujigiwa

Katase

Lefeuvre

et al. 2013

J Oral Pathology Medicine

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Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix-forming, GFP Rat-derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet-like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with β-TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp-derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.

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Histopathological Study of Matrix Mineralization by Osteoblastic-like and Odontoblastic-like Cells in Diffusion Chamber

Cited by 2 publications

References 10 publications

Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier

Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier

Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro

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