Historical remarks on the Henderson-Hasselbalch equation: its advantages and limitations and a novel approach for exact pH calculation in buffer region

Radić, Njegomir; Prkić, Ante

doi:10.1515/revac-2012-0001

Cited by 14 publications

(10 citation statements)

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“…They represent typical Henderson-Hasselbalch plots. 25 Several reports have shown similar calibration curves for the pH dependence of SERS signals of MBA but they usually estimate pH i values by comparing calibration spectra with SERS spectra from cells. 11,13,15,17 Therefore, we additionally fitted an equation to the curves to give a simple mathematical formulation for the determination of pH based on spectral changes of 4-mercaptobenzoic acid, see Table 1.…”

Section: Results and Discussion Ph Calibration Curvesmentioning

confidence: 99%

SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

Jaworska

Jamieson

Malek

et al. 2015

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The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-α (TNFα). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments.

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Section: Results and Discussion Ph Calibration Curvesmentioning

confidence: 99%

SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

Jaworska

Jamieson

Malek

et al. 2015

Analyst

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“…However, eq is applicable to a limited range of solution conditions and does not take into account nonideal behavior and ion activity. This results in a breakdown of the H–H equation for relatively strong acids or bases as well as for systems where activity coefficients cannot be neglected. , The Debye–Hückel theory (DHT) and specific ion interaction theory (SIT) can be used to calculate ion activities. Both of these approaches more accurately determine pH by taking into account deviations from ideal solution behavior by calculating activity coefficients for strong electrolyte solutions. ,, …”

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confidence: 99%

Titration of Aerosol pH through Droplet Coalescence

Coddens

Angle

Grassian

2019

J. Phys. Chem. Lett.

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The pH of aqueous aerosols, as well as cloud and fog droplets, has an important influence on the chemistry that takes place within these unique microenvironments. Utilizing conjugate acid/base pairs to infer pH changes, we investigate, for the first time, changes in aerosol pH upon coalescence. In particular, we show that the pH within individual aqueous aerosols that are ∼8 μm in diameter can be titrated via droplet coalescence in an aerosol optical tweezer. Using sulfate/bisulfate and carbonate/bicarbonate as model systems, the pH of trapped aerosols is determined before and after introduction of smaller aerosols containing a strong acid. The pH change upon coalescence with the smaller, acidic aerosol is calculated using specific ion interaction theory. Furthermore, we show that the pH of an individual aerosol can be altered along a fairly wide range of pH values, paving the way for future studies requiring rigorous pH control of an aqueous aerosol.

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“…Sulfate/bisulfate has been used most frequently. The pH can be determined by substituting the conjugate ion pair concentrations and its dissociation coefficient into Henderson-Hasselbalch (H−H) (Radić and Prkić, 2012), Debye-Hückel theory, and spe-cific ion interaction theory equations, respectively (Ciavatta, 1980;Rindelaub et al, 2016;Craig et al, 2017Craig et al, , 2018Coddens et al, 2019;Chang et al, 2020a). The latter two are more suitable to supersaturated particles since non-ideality has been considered.…”

Section: Determination Of Phmentioning

confidence: 99%

Single-particle Raman spectroscopy for studying physical and chemical processes of atmospheric particles

et al. 2022

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Abstract. Atmospheric particles experience various physical and chemical processes and change their properties during their lifetime. Most studies on atmospheric particles, both in laboratory and field measurements, rely on analyzing an ensemble of particles. Because of different mixing states of individual particles, only average properties can be obtained from studies using ensembles of particles. To better understand the fate and environmental impacts of atmospheric particles, investigations on their properties and processes at a single-particle level are valuable. Among a wealth of analytic techniques, single-particle Raman spectroscopy provides an unambiguous characterization of individual particles under atmospheric pressure in a non-destructive and in situ manner. This paper comprehensively reviews the application of such a technique in the studies of atmospheric particles, including particle hygroscopicity, phase transition and separation, and solute–water interactions, particle pH, and multiphase reactions. Investigations on enhanced Raman spectroscopy and bioaerosols on a single-particle basis are also reviewed. For each application, we describe the principle and representative examples of studies. Finally, we present our views on future directions on both technique development and further applications of single-particle Raman spectroscopy in studying atmospheric particles.

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Historical remarks on the Henderson-Hasselbalch equation: its advantages and limitations and a novel approach for exact pH calculation in buffer region

Cited by 14 publications

References 0 publications

SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

Titration of Aerosol pH through Droplet Coalescence

Single-particle Raman spectroscopy for studying physical and chemical processes of atmospheric particles

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