History and New Developments of Assays for Cholinesterase Activity and Inhibition

Butyrylcholinesterase as a biochemical marker

“…Indoxylacetate is suitable for both AChE and BChE assay but the turnover number is 2.65 times higher for BChE than for AChE (82). Indoxylacetate was proven to be suitable for construction of disposable sensors for a fast assay of cholinesterase´s inhibitors using cholinesterase as a biorecognition element (83,84) 2,6-dichloroindophenyl acetate is another substrate providing a compound measurable spectrophotometrically and fl uorimetrically (85,86). The formed 2,6-dichloroindole is red in acidic conditions.…”

Modified Cholinesterase Technology in the Construction of Biosensors for Organophosphorus Nerve Agents and Pesticides Detection

“…The simplest one is based on detecting the acid increment by means of a pH-metric device with continuous recording. This configuration, however, is not that suitable for the use in field conditions (Miao et al, 2010). Potentiometric detectors were also constructed for both hydrolases substrates, Ach and BuCh.…”

“…The conductometric biosensors have a big advantage because they do not require the reference electrode and the converter provides the possibility of miniaturization (Miao et al, 2010). It is impossible to analyze various mixtures of analytes in real samples using just one biosensor type.…”

“…Reducing the fluorescent signal of the pH indicator is proportional to the concentration of pesticides tested. This biosensor was tested for detection of the total toxicity in samples of drinking water (Vamvakaki & Chaniotakis, 2007; as cited in Miao et al, 2010). Using the fluorescent substances for detection of cholinesterases inhibitors has found its way also to analytical kits.…”

Preparação de derivados triptamínicos e dímeros inibidores de acetilcolinesterase a partir da bufotenina isolada de Anadenanthera (Fabaceae: Mimosideae)

“…48 Uma abordagem inovadora e interessante para determinação da atividade inibitória da AChE é a técnica de imobilização enzimática em bioreator capilar (ICER, immobilized capillary enzyme reactor) em sistema de fluxo, cromatografia líquida (CL), acoplado a espectrofotometria UV-vis 49 e espectrofometria de massa. 50 A técnica permite a quantificação em fluxo do produto da catálise enzimática da acetilcolinesterase sobre a acetilcolina, substrato natural da enzima.…”