2014
DOI: 10.1074/jbc.m114.554089
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HIV-1 Envelope Proteins and V1/V2 Domain Scaffolds with Mannose-5 to Improve the Magnitude and Quality of Protective Antibody Responses to HIV-1

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Cited by 22 publications
(35 citation statements)
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“…Although the gp120 expression data presented here was derived exclusively from small-scale transient transfection experiments, we anticipate that the cell line described in this report will be useful for the development of stable MGAT1 - CHO cell lines producing vaccines based on a variety of new concepts. These include guided immunization to stimulate germline genes encoding bNAbs (79–81), Env proteins designed with features that enhance antigen processing and presentation (82) as well as glycopeptide scaffolds that enhance the immunogenicity of epitopes recognized by bN-mAbs while eliminating non-protective immunodominant epitopes (21).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Although the gp120 expression data presented here was derived exclusively from small-scale transient transfection experiments, we anticipate that the cell line described in this report will be useful for the development of stable MGAT1 - CHO cell lines producing vaccines based on a variety of new concepts. These include guided immunization to stimulate germline genes encoding bNAbs (79–81), Env proteins designed with features that enhance antigen processing and presentation (82) as well as glycopeptide scaffolds that enhance the immunogenicity of epitopes recognized by bN-mAbs while eliminating non-protective immunodominant epitopes (21).…”
Section: Discussionmentioning
confidence: 99%
“…Previously we reported that the same gp120s used in the RV144 trial could be modified to bind multiple bN-mAbs when expressed in a cell line (HEK 293 GnTI - ) that limited N-linked glycosylation to Man 5 , or earlier species (e.g. Man 8 , Man 9 ) (21). While in theory this cell line could be used to produce a glycan optimized gp120 vaccine, in reality this is not practical.…”
Section: Introductionmentioning
confidence: 99%
“…These rabbit experiments are among the first to demonstrate that the immune response can be focused on the V1V2 region (54). The cross-reactivity of human V1V2 Abs has long been established although this region has the most sequence and length diversity in the HIV-1 Env (22).…”
Section: Discussionmentioning
confidence: 99%
“…38 Recent results have also illustrated that some of the bnAbs such as PG9 and PG16 require part of peptide segment of the antigen to bind to. 15,39,40 We envision that this peptide portion could be coupled to glycopolymers generated from monomer 2 (Scheme 3) by investigating end-functionalization of the polymers during the quenching stage of ROMP with an amine group, which will subsequently serve as an attachment point. 22 …”
mentioning
confidence: 99%