HIV-1 Inhibiting Capacity of Novel Forms of Presentation of GB Virus C Peptide Domains is Enhanced by Coordination to Gold Compounds

Gómara, María J.; Galatola, Ramona; Gutiérrez, Alejandro García; Gimeno, María C.; Gatell, José M.; Sánchez-Merino, Víctor; Yuste, Eloísa; Haro, Isabel

doi:10.2174/09298673113206660276

Cited by 9 publications

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“…[ 38 ] The high prevalence of HPgV among HIV-1 individuals has been reported in several studies in Brazil and the world [ 42 – 44 ]. The association between the presence of HPgV and HIV is owing to the fact that HPgV likely acts as a protective factor for the development of HIV [ 44 – 46 ].…”

Section: Discussionmentioning

confidence: 99%

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Silva

Barata

et al. 2020

Virol J

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Background Human pegivirus (HPgV)—formerly known as GBV-C—is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Belém-PA-Brazil. Methods Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. Results The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. Conclusions This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon.

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Section: Discussionmentioning

confidence: 99%

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Silva

Barata

et al. 2020

Virol J

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“…The high prevalence of HPgV among HIV-1 individuals has been reported in several studies in Brazil and the world [40][41][42]. The association between the presence of HPgV and HIV is owing to the fact that HPgV likely acts as a protective factor for the development of HIV [40,43,44].…”

Section: Discussionmentioning

confidence: 89%

Human pegivirus (HPgV, GBV-C) RNA prevalence, genome characterization and association with HIV coinfection among volunteer blood donors from a public hemotherapy service in Northern Brazil.

Silva

Barata

et al. 2020

Preprint

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BackgroundHuman pegivirus (HPgV) - formerly known as GBV-C - is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Belém-PA-BrazilMethodsPlasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome+. Genome characterization and phylogenetic analysis were conducted.ResultsThe prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 to 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The genotype 2 (2A and 2B) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution.ConclusionsThis study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time the genotype 2 genomes in the Brazilian Amazon region.

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“…With this aim, 2 different PEG spacers were chosen: a short one composed of 3 units of ethylene oxide (PEG 3 ) and a longer polyoxyethylene one composed of 27 units (PEG 27 ). On the other hand, and based on our previous results where we demonstrated that the lipophilic conjugation on N -terminus was able to enhance the antiviral activity of the FI peptides, 24 we selected the N -terminal end of the peptide sequence to obtain the 6 PAs illustrated in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

Peptide Amphiphilic-Based Supramolecular Structures with Anti-HIV-1 Activity

et al. 2021

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In a previous work, we defined a novel HIV-1 fusion inhibitor peptide (E1P47) with a broad spectrum of activity against viruses from different clades, subtypes, and tropisms. With the aim to enhance its efficacy, in the present work we address the design and synthesis of several peptide amphiphiles (PAs) based on the E1P47 peptide sequence to target the lipid rafts of the cell membrane where the cell–cell fusion process takes place. We report the synthesis of novel PAs having a hydrophobic moiety covalently attached to the peptide sequence through a hydrophilic spacer of polyethylene glycol. Characterization of self-assembly in condensed phase and aqueous solution as well as their interaction with model membranes was analyzed by several biophysical methods. Our results demonstrated that the length of the spacer of polyethylene glycol, the position of the peptide conjugation as well as the type of the hydrophobic residue determine the antiviral activity of the construct. Peptide amphiphiles with one alkyl tail either in C-terminus ( C -PA monoalkyl ) or in N-terminus ( N -PA monoalkyl ) showed the highest anti-HIV-1 activities in the cellular model of TZM-bl cells or in a preclinical model of the human mucosal tissue explants.

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HIV-1 Inhibiting Capacity of Novel Forms of Presentation of GB Virus C Peptide Domains is Enhanced by Coordination to Gold Compounds

Cited by 9 publications

References 0 publications

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Human pegivirus (HPgV, GBV-C) RNA prevalence, genome characterization and association with HIV coinfection among volunteer blood donors from a public hemotherapy service in Northern Brazil.

Peptide Amphiphilic-Based Supramolecular Structures with Anti-HIV-1 Activity

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