HIV Antibody EIA and Western Blot Assay Results on Paired Serum and Saliva Specimens

Fitzgibbons, D; Barriga, Gonzalo P.; Seymour, Emily; Stramer, Susan L.

doi:10.1111/j.1749-6632.1993.tb18375.x

Cited by 6 publications

(3 citation statements)

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“…The sensitivity of the EIAs used in this study was 100%, which is comparable to observed performance (95 to 100%) now being reported in most studies of EIA testing of OF (1,5,8,9,11,12,19,23,29,30). OF specimens collected with the Omni-Sal and other collection devices are of better quality and greater volume than those collected by the expectoration or salivary-induction procedures formerly used.…”

Section: Discussionsupporting

confidence: 85%

“…However, improving WB detection of HIV-1-specific antibodies in OF has proven more difficult. Current WB testing of OF specimens relies on the strip methods designed for serum testing, and these methods have generally performed poorly with OF because of the volume of sample required and the dilution of the specimen (1,4,12,20,29,32). Thus, confirmatory testing for OF has relied mainly on in-house assays or line immunoassays to achieve results similar to those obtained with serum WB (6,24,32).…”

mentioning

confidence: 99%

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Oral fluid as a specimen for detection and confirmation of antibodies to human immunodeficiency virus type 1

Granade

Phillips

Parekh

et al. 1995

Clin Diagn Lab Immunol

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Paired serum and oral fluid specimens (n ‫؍‬ 287) were collected with the Omni-Sal device and were assayed for the presence of antibodies to human immunodeficiency virus type 1 (HIV-1). Enzyme immunoassays (EIAs)-Abbott 3A11, an Organon Teknika Corporation research-use-only test, and the Murex GACELISAwere used per the manufacturers' inserts or were modified slightly to accommodate the oral fluid specimens. Compared with serum Western blot (immunoblot) results, each EIA had a sensitivity of 100% and the specificities were 89.6% for the Abbott 3A11 EIA, 96.5% for the GACELISA, and 97.8% for the Organon Teknika Corporation EIA. Specificities based on specimens that were repeatedly reactive were 99.3% for all EIAs. A miniaturized Western blot technique used for confirmatory testing of both the serum and oral fluid specimens found 149 of the 287 samples to be HIV-1 antibody positive in both sample types. The Western blot banding patterns observed for the serum and oral fluid specimens were essentially identical. Immunoglobulin G concentrations were determined for all oral fluid specimens and ranged from <0.5 to >40.0 g/ml. Immunoglobulin G concentrations did not correlate with the ability of any of the EIAs to detect HIV-1-specific antibody or with the ability of the modified Western blot to detect HIV-1 protein-specific antibodies.

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Section: Discussionsupporting

confidence: 85%

mentioning

confidence: 99%

Oral fluid as a specimen for detection and confirmation of antibodies to human immunodeficiency virus type 1

Granade

Phillips

Parekh

et al. 1995

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

show abstract

“…The relatively high specificity obtained by most assays using oral fluids supports the use of oral fluids as an epidemiological tool (7,14,17). Six of the 15 (18-32) most recent studies included 11 evaluations of EIA assays (some evaluations used the same EIA), with only four of the 11 EIAs having a sensitivity of < 98% (range 93-96%) (20,22,23,27) and specificities generally between 98-100%. The less than optimal test indices from these studies were most likely due to the use of tests designed for use with serum samples, not oral fluids.…”

Section: Discussionmentioning

confidence: 94%