HLA‐A, ‐B and ‐DRB1 allele frequencies in the Bangladeshi population

Abstract

Population genetic studies have become an invaluable tool because of the extreme polymorphism found at some of the loci of the human leukocyte antigen (HLA) system. In this study, we are reporting for the first time the genetic polymorphism of 141 healthy unrelated Bangladeshi Bangalees living in central region of Dhaka. We studied the HLA-A, -B and -DRB1 loci using polymerase chain reaction with sequence-specific primers. The allelic frequencies, two and three locus haplotype frequencies were statistically analyzed. A total of 16 HLA-A alleles, 26 HLA-B alleles and 14 HLA-DRB1 alleles were detected. A*33-B*44 (8.15%) was the most common two loci class 1 haplotype, whereas A*33-B*44-DRB1*07 (6.38%) was the most frequent three loci haplotype. The most common HLA-A, HLA-B and HLA-DRB1 alleles were A*33 (17.02%), B*15 (19.5%) and DRB1*15 (29.07%), respectively. Construction of phylogenetic tree using average linkage between groups and correspondence analysis showed close associations with Indian non-tribal random Dravidians, north Indian Hindus and some relations with Mongolian and Pakistani populations. We believe this data will provide useful information for bone marrow registry, legal medicine, disease association and anthropological studies.

Human Leukocyte Antigen: Class I Allele Frequencies and Haplotype Distributionin a Tertiary Care Hospital in Bangladesh

“…However, in a previous study from Bangladesh carried out among 141 samples, A11 was detected as the third highest frequent allele. 6 This variation of HLA A frequency between the present and previous study may be due to disproportion between the sample size of these two studies, as the earlier study was done on only 141 samples, while the present study had a much larger sample size of 1070. When compared with world population, A11 is found to be less frequent in Cuba, Argentina, Kenya and among Black and Hispanic ethnic regions.…”

“…25 In this neighboring region in Siliguri (West Bengal), the two locus haplotype analysis revealed that A1-B37, A1-B40, A29-B40, A30-B51 and A31-B40 were the most frequent haplotypes. 26 In a previous study in Bangladesh, A33-B44 was found to be the most frequent allele, 6 but this was observed as the sixth frequent allele in our study. Thus, the two locus haplotypes A11: B75 revealed that the Bangladeshi population belongs to the Asian population and is most closely related to Oriental groups than to European groups.…”

“…When compared with world population, A11 is found to be less frequent in Cuba, Argentina, Kenya and among Black and Hispanic ethnic regions. The second most frequent allele in this study, A24, was the second highest allele 6 and the most common allele among the Dravidian tribal communities of South India 27 and the northern Indian populations. 28 A24 was also observed in Srilanka, India (Kerala), Pakistan, Taiwan, Japan, Caucasoid, Hispanic, Iraqi kurdish people, but less frequently among black people.…”

“…9 HLA profiles of Bengali people and some of the ethnic and tribal populations are available from different parts of India [15][16][17] but so far, there is only one study on HLA profile among 141 Bangladeshi population living in Dhaka and HLA-A, HLA-B and HLA-DRB1 were determined using polymerase chain reaction with sequence-specific primers. 6 Thus, the present study was aimed to obtain information concerning the distribution of HLA class I antigens in the Bangladeshi population and to compare the results with other populations. Sample collection and laboratory methods: Fresh blood (5 ml) was collected in heparin containing tube and the laboratory test was performed by the microlymphocytotoxicity technique according to manufacturer's instructions.…”

“…5 Thus, the history of Bangladesh combines Indo-Aryan, Austro-Asiatic, Dravidian, Mughal, Arab, Persian, Turkic and British influences. 6 There are several ethnic groups in Bangladesh, where 98% are Bengali and the remaining 2% belong to the Chakma, Bawm, Garo, Kiri, Marma, Tipuri and other ethnic minorities. The Bengali population are natively concentrated in Bangladesh and in the Indian states of West Bengal, Tripura and Assam's Barak valley.…”