HLA‐A, ‐B, and ‐DRB1 polymorphism defined by sequence‐based typing of the Han population in Northern China

Abstract

DNA typing for human leukocyte antigen (HLA)-A, -B and -DRB1 was performed using polymerase chain reaction-sequence-based typing method on 618 randomly selected healthy individuals of the Han population in Northern China. Allele frequencies and haplotypes were statistically analyzed. A total of 84 HLA-A alleles, 143 B alleles, and 122 DRB1 alleles were detected, and 853 A-B-DRB1 haplotypes, 473 A-B haplotypes, and 551 B-DRB1 haplotypes were statistically inferred. Statistical analysis of three-locus haplotypes showed that A*0207-B*4601-DRB1*0901 (3.06%) was the most predominant. Gene frequencies and haplotypic associations within HLA-A, -B, and -DRB1 loci were determined at a high-resolution (four digit) allelic level and should provide useful information in anthropology, bone marrow donor registry, legal medicine, and disease association studies.

High-resolution human leukocyte antigen (HLA) haplotypes and linkage disequilibrium of HLA-B and -C and HLA-DRB1 and -DQB1 alleles in a Taiwanese population

“…A comparison of HLA-A, -B, and -DRB1 haplotypes determined in the Taiwanese population with that in the Han population in northern China [22] yielded comparable results. Seven of the 10 most common HLA-A, -B, and -DRB1 haplotypes predicted in the Taiwanese population were among the 10 most popular haplotypes in the northern Han Chinese population, reaffirming a relatively close relationship between the Taiwanese and the northern Han Chinese with regard to HLA genes.…”

Human leukocyte antigen-A, -B, and -DRB1 haplotypes of cord blood units in the Tzu Chi Taiwan Cord Blood Bank

“…We compared our predicted haplotypes with the results of the haplotype study on Singapore Chinese CB units [15] and other population studies [10,[13][14][15][16]. Thirty-two of the 34 haplotypes (94%) determined in Singapore Chinese CB units that occurred more than once in the study were observed in our current investigation (Supplementary Table 1).…”

Distributions of human leukocyte antigen–A, –B, and –DRB1 alleles and haplotypes based on 46,915 Taiwanese donors

“…Comparison of populations was limited by the availability of allele-level data for HLA-A, -B, and -DRB1 loci. We chose 10 populations, including Korean [18], Western Javanese [20], Japanese [22], Vietnamese [23], Maonan in China [24], Southern Chinese [25,26], Northern Chinese [27], North African immigrants in France, French, and Eastern European Americans [28] for the reason that their donors were HLA typed with the same level of resolution in the HLA-A, -B, and -DRB1 loci as our donors. We performed the PCA and clustering analysis based on 21 commonly shared HLA-A, -B, and -DRB1 alleles and their corresponding frequencies for the two Taiwanese groups and the 10 chosen worldwide populations.…”