HLA-B27 subtypes determination in patients with ankylosing spondylitis from Zulia, Venezuela

Cipriani, Adriano; Rivera, Sergio; Hassanhi, Manzur; Márquez, Georgina; Hernández, Rosaura; Villalobos, Cecilia; Montiel, Milagros

doi:10.1016/s0198-8859(03)00085-5

Cited by 33 publications

(18 citation statements)

References 14 publications

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“…In the same way, B*2708 was present in one case in our study. It is a rare subtype that was first observed in Britain and has been reported in association with AS in a large family from Azores Islands and was described to have a potential protective role in the Mestizo population (Venezuela) (32). The other interesting finding in our study is the positive association of AS and HLA-Cw02 because its frequency was significantly higher in AS patients (26.6%) than controls (7.5%).…”

Section: Discussionsupporting

confidence: 62%

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Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*

et al. 2015

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Ankylosing spondylitis (AS) is very often associated with human leukocyte antigen (HLA), particularly HLA-B*27. However, the strength of this association and clinical features may vary in different ethnic groups. Our study aims to assess the distribution of HLA-A, -B, -Cw and DRB1 alleles in Moroccan patients with AS and to compare the clinical features of AS and the frequencies of HLA-B27 in patients from Morocco with other series. Seventy-five patients diagnosed with AS and assessed for clinical manifestations were selected and compared to 100 healthy controls. HLA class I and II antigens were typed by polymerase chain reaction sequence-specific oligonucleotide. HLA-B27 subtypes were studied by polymerase chain reaction amplification with sequence-specific primers. HLA-B27 was found in 64% of patients. It was positively associated with younger age at disease onset, family history, and uveitis while it had a negative association with late onset. Six B*27 subtypes were identified in the AS group. HLA-B*2705 and B*2702 were the most common observed subtypes. Among other HLA genes, a significant increase in the prevalence of HLA-Cw*02 and HLA-DRB*15 was found in AS patients. HLA-B27 is involved in the predisposition of AS in the Moroccan population. HLA-B*2705 and B*2702 were the predominant subtypes supporting previous reports in Caucasian spondyloarthropathies. Other HLA genes, HLA-Cw*02 and HLA-DRB1*15, seem to confer predisposing effect to the disease. However, the lower frequency of HLA-B27 compared to the literature in our study suggests the existence of different genetic and/or environmental factors in Morocco.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*

et al. 2015

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“…This allele is different from B*2705 allele in only one amino acid in the α1 domain at position 59 (23). The observed frequency of B*2704 allele in our population is similar to the frequency of that allele among Jews (24). Interestingly, two more subtypes, B*2708 and B*2714 alleles, were each detected in one individual.…”

Section: Discussionsupporting

confidence: 72%

The study of the extended haplotypes of rare HLA‐B*2730 allele using microsatellite loci

et al. 2008

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The aim of the present study was to compare haplotypes of the most frequent B*27 alleles among Croatians (B*2702 and *2705) and the rare B*2730 allele. For this purpose, 37 families with members carrying human leukocyte antigen (HLA)-B27 were selected. All individuals were analysed for eight microsatellites (Msats): D6S2927, short tandem repeat - MHC class I-related gene (STR_MICA), D6S2793, D6S2811, tumor necrosis factor a (TNFa), tumor necrosis factor d (TNFd), D6S273 and D6S1014, while individuals carrying the HLA-B27 specificity were subtyped. Of 39 analysed haplotypes, 20 individuals had B*2702, 15 subjects were positive for the B*2705 allele, the B*2730 allele was found in three haplotypes from different families, while one individual carried the B*2703 allele. HLA-A3 and -DRB1*16 were shared by all three B*2730 haplotypes. The DRB1*16 allele was also observed in the majority of B*2702 haplotypes (76.5%), while HLA-A3 was, after HLA-A2, the second most frequent HLA-A specificity in B*2702 haplotypes. No such correlation was found for the B*2705 haplotypes. Msat analysis showed that B*2730 haplotypes also share the same allele at all tested Msats. The D6S2927, D6S2793, MICA and TNFd Msats were not useful in distinguishing B*2702 and B*2705 alleles because D6S2927-213bp, STR_MICA-179bp, D6S2793-206bp, D6S2811-83bp and TNFd-130bp were detected in almost all cases. Conversely, for the TNFa, D6S273 and D6S1014 loci, haplotypes carrying B*2702 and B*2730 shared a single Msat allele in the majority of cases (TNFa-113bp, D6S1014-134bp and D6S273-134bp), which was not observed for B*2705 haplotypes. In conclusion, the similarity between B*2702 and B*2730 DNA sequences as well as their sharing of the same haplotypic combinations corroborates the proposed mechanism of B*2730 evolution from B*2702 by interallelic recombination.

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“…In addition to B*27:06 and B*27:09, other HLA-B27 alleles such as B*27:08 in Venezuela and B*27:07 in Cyprus were claimed not to be associated with AS (Armas et al, 1999). But these results are not universal, in other populations the same alleles have been found in AS patients (Cipriani et al, 2003;Varnavidou-Nicolaidou et al, 2004;Paladini et al, 2005). (Table 1) B27 …”

Section: Structure Subtypes and Epidemiology Of Hla-b27mentioning

confidence: 84%

HLA-B27 and Ankylosing Spondylitis

Tsai¹

2012

Clinical and Molecular Advances in Ankylosing Spondylitis

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HLA-B27 subtypes determination in patients with ankylosing spondylitis from Zulia, Venezuela

Cited by 33 publications

References 14 publications

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*

The study of the extended haplotypes of rare HLA‐B*2730 allele using microsatellite loci

HLA-B27 and Ankylosing Spondylitis

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HLA-B27 subtypes determination in patients with ankylosing spondylitis from Zulia, Venezuela

Cited by 33 publications

References 14 publications

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B*27

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B*27

The study of the extended haplotypes of rare HLA‐B*2730 allele using microsatellite loci

HLA-B27 and Ankylosing Spondylitis

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Product

Resources

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Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*

Polymorphisms of HLA‐A, ‐B, ‐Cw and DRB1 antigens in Moroccan patients with ankylosing spondylitis and a comparison of clinical features with frequencies of HLA‐B27*