HLA-DR Alleles are Associated with IDDM, but not with Impaired Neutrophil Chemotaxis in IDDM

Abstract

Insulin-dependent diabetes mellitus (IDDM) is a risk factor for periodontitis. Depressed neutrophil chemotaxis has been demonstrated in IDDM and in early-onset periodontitis (EOP). HLA-DR antigens are associated with both IDDM and periodontitis. This investigation sought to determine an association of HLA-DR3, -DR4, and -DR53 with impaired neutrophil chemotaxis in an IDDM sample. The neutrophil chemotaxis index of 41 diabetics and 27 controls was determined by a modified Boyden chamber method, and certain class II HLA genotypes were determined by polymerase chain-reaction amplification of genomic DNA by means of sequence-specific primers (PCR-SSP). The mean chemotaxis index of the diabetics was significantly less than that of the controls (p < or = 0.02). HLA-DR3 (p < or = 0.002), -DR4 (p < 0.003), and -DR53 (p < or = 0.001) were associated with IDDM. Neutrophil chemotaxis and glucose metabolism were not significantly correlated. None of the HLA-DR alleles was associated with impaired neutrophil chemotaxis. Therefore, the neutrophil chemotaxis defect of IDDM appears to be independent of these HLA-DR-associated genes.

Localized Aggressive Periodontitis in a Patient With Type 1 Diabetes Mellitus: A Case Report

“…35 Type 1 DM has been associated with certain HLA phenotypes. 36,37 Its association with specific HLA phenotypes, especially HLA DR4, has also supported a genetic basis for aggressive periodontitis. 38 In the present case, HLA markers of the patient were analyzed to determine if these markers might serve as an indicator for disease susceptibility.…”

The effect of non‐surgical periodontal therapy on peroxidase activity in diabetic patients: a case–control pilot study

“…PMN plays an important role in the maintenance of periodontal health against bacterial challenge. Nevertheless, the occurrence of dysfunctions in these cells with respect to chemotaxis, adherence and phagocytosis has been observed in diabetic subjects (Manouchehr‐Pour et al 1981, Marhoffer et al 1992, Sawant 1993, Delamaire et al 1997, Gustke et al 1998), which may be related to the inadequate metabolic control of diabetes mellitus (Wierusz‐Wysocka et al 1993).…”

Periodontitis and type II diabetes: a two-way relationship

“…Diabetic patients have depressed polymorphonuclear neutrophils chemotaxis, as well as defective polymorphonuclear neutrophils apoptosis . Furthermore, neutrophils from diabetic individuals have increased production of superoxide, which enhances oxidative stress, hence more destruction of periodontal tissues .…”

“…It is well recognised that neutrophil defects that impair the clearance of bacteria also result in an increased risk for periodontitis. 84,85 Diabetic patients have depressed polymorphonuclear neutrophils chemotaxis, 86,87 as well as defective polymorphonuclear neutrophils apoptosis. 80,82,88,89 Furthermore, neutrophils from diabetic individuals have increased production of superoxide, which enhances oxidative stress, hence more destruction of periodontal tissues.…”