Hmob3 brain-specific sequence is a part of phylogenetically conserved human MAP1B gene 3′-untranslated region

Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity

“…Primers specific to CPLX2 were designed using OLIGO Primer Analysis software (Wojciech and Piotr Rychlik Copyright, version 6.31); the primer sequences and nucleotide coordinates are summarized in Table 1. PCR amplifications were performed in 20 AL of the reaction mix, as described previously (Dergunova et al, 2003b) containing 1 AL of reverse transcription reaction mix as a template. PCR parameters were as follows: 5 min at 95 -C followed by 30 cycles of 95 -C for 1 min, 65 -C for 1 min, and 72 -C for 1 min, followed by a 10 min final elongation at 72 -C. Aliquots of the PCR products were size-fractionated in 6% nondenaturing polyacrylamide gels in Tris -Borate-EDTA buffer.…”

Human gene MOB: structure specification and aspects of transcriptional activity

“…First strand cDNA was used in the amplification with the combination of the same upstream primer F1 and different downstream primers R1, R2, R3, R5, R6, R7 and R8 (Table 2). PCR was performed in 20 Al of the reaction mix described previously (Dergunova et al, 2003) containing 1 Al of reverse transcription reaction mix. PCR parameters were as follows: 4 min at 94 jC followed by 30 cycles of 94 jC for 1 min, 65 jC for 1 min and 72 jC for 1 min followed by a 10-min final elongation at 72 jC.…”