Hole‐burning Spectroscopy of Active and Inactivatee) Photosystem Ii Particles

Abstract

This paper reports transient and persistent hole‐burning of photosynthetically active as well as chemically reduced and heat inactivated photosystem II particles isolated from cyanobacteria. Transient spectra of active and non‐active particles are significantly different. For both, the possible origin of the bottle‐neck state is discussed. Persistent holes were ascribed to the antenna complex of photosystem II. From their width the energy transfer rate was estimated to be 4.8 ps at 4.2 K.

Gaussian Decomposition of Absorption and Linear Dichroism Spectra of Outer Antenna Complexes of Photosystem II

“…The other four complexes (LHCII, CP29, CP26, CP24), all of which contain chi b in addition to chi a, form the so-called outer antenna. On the basis of biochemical studies several structural models have been presented which describe the The absorption spectrum of PSII antenna, as is the case also for PS I antenna, is modified by a coarse-grained inhomogeneous broadening associated with the presence of a number of different chi electronic transitions (Hayes et al, 1988;Ikegami & Itoh, 1988;Gillie et al, 1989;Zucchelli et al, 1990;Vacha et al, 1991;Van der Vos et al, 1991;Hemelrijk et al, 1992). One way which is commonly used to analyze this is that of spectral decomposition into Gaussian sub-bands (French et al, 1972; Dorssen et al, 1987b; Ikegami & Itoh, 1988;Zucchelli et al, 1990Zucchelli et al, , 1992Van der Vos et al, 1991;Hemelrijk et al, 1992;Holzwarth, 1992;Jennings et al, 1993b;Trissl et al, 1993).…”