Hollowfiber Polyurethane-Collagen Coating Chitosan as Nerve Graft for Therapy of Peripheral Nerve Injury in Extreme Paralysis

Abstract

Peripheral nerve injury with gaps between 5 and 30 mm can result in permanent paralysis because axons are cut. The distance between axons, which is more than 1-2 cm, needs graft in the form of nerve connecting pipe in order to repair the defects. A synthesis of hollowfiber polyurethane-collagen coated by chitosan was carried out to identify its potential as treatment accelerator for peripheral nerve injury. The result of Fourier Transform Infrared (FTIR) analysis showed multiple links between chitosan and glutaraldehyde, which can be seen in wavenumber shift from 1080-1100 cm-1 to 1002 cm-1. The degradation test result revealed that the sample displayed mass loss after it was soaked in simulated body fluid(SBF) for seven days. Polyurethanecan be degraded in the body after 30 days. This converges with the nerve mechanism that regenerates at the rate of 1 mm/day or 1 inch/month. The result of tensile test indicated that modulus values of chitosan coating variation of 1%, 1.5%, and 2% were 4.75 MPa, 4.74 MPa, and 7.67 Mpa respectively. The outcome of scanning electron microscope(SEM)showed that hollow fiberhas a diameter of 2.021-2.032 mm, which matches the diameter of peripheral nerves ranging from 1.5 to 3 mm and the membrane pore size of 31.33-39.65 μm. The result of MTT assay demonstrated that the percentage of viable fibroblastBHK-21cells was exceeding 50%, which means that the sample does not have toxic properties. The result of this study is expected to provide theoretical basis for the utilization of polyurethane-collagen coating chitosan as nervegraft for theraphy of peripheral nerve injury. The utilization is possible due to the fact that the composite exhibits biocompatible, regenerative, and easily degradable characteristics. Moreover, it could become an alternative solution to answer the need of a more affordable and easier-to-produce nerve graft, so it can be mass-produced in Indonesia.

Facile preparation of degradable multi-arm-star-branched waterborne polyurethane with bio-based tannic acid

“…The weight loss ratio was calculated using the eqn (3) , where W 0 and W t are the weights of the samples before and after degradation. 17,18 …”

“…where W 0 and W t are the weights of the samples before and aer degradation. 17,18 Biodegradation study of multi-arm-star-branched WPU in soil…”

The Clinical Function Comparison of Post Operative Nerve Grafting and Nerve Transfer in Patients with Brachial Plexus Injury

“…The success of nerve reconstruction with micro surgical techniques should be followed by the assessment of patients' clinical function as postoperative follow-up 8 both oriented to surgeons such as range of movement (ROM) measurement of joint activation and motor strength recovery, as well as patient-oriented assessment such as neuropathic pain severity and upper limb dysfunction 9 . From that statement, the aim of this study to compare the clinical function of post-operative nerve grafting with nerve transfer in patients with brachial plexus injury 10 .…”