Electrostimulation and electroactive scaffolds can positively influence and guide cellular behaviour and thus has been garnering interest as a key tissue engineering strategy. The development of conducting polymers such as polyaniline enables the fabrication of conductive polymeric composite scaffolds. In this study, we report on the initial development of a polycaprolactone scaffold incorporating different weight loadings of a polyaniline microparticle filler. The scaffolds are fabricated using screw-assisted extrusion-based 3D printing and are characterised for their morphological, mechanical, conductivity, and preliminary biological properties. The conductivity of the polycaprolactone scaffolds increases with the inclusion of polyaniline. The in vitro cytocompatibility of the scaffolds was assessed using human adipose-derived stem cells to determine cell viability and proliferation up to 21 days. A cytotoxicity threshold was reached at 1% wt. polyaniline loading. Scaffolds with 0.1% wt. polyaniline showed suitable compressive strength (6.45 ± 0.16 MPa) and conductivity (2.46 ± 0.65 × 10−4 S/cm) for bone tissue engineering applications and demonstrated the highest cell viability at day 1 (88%) with cytocompatibility for up to 21 days in cell culture.

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Peripheral nerve injury with gaps between 5 and 30 mm can result in permanent paralysis because axons are cut. The distance between axons, which is more than 1-2 cm, needs graft in the form of nerve connecting pipe in order to repair the defects. A synthesis of hollowfiber polyurethane-collagen coated by chitosan was carried out to identify its potential as treatment accelerator for peripheral nerve injury. The result of Fourier Transform Infrared (FTIR) analysis showed multiple links between chitosan and glutaraldehyde, which can be seen in wavenumber shift from 1080-1100 cm-1 to 1002 cm-1. The degradation test result revealed that the sample displayed mass loss after it was soaked in simulated body fluid(SBF) for seven days. Polyurethanecan be degraded in the body after 30 days. This converges with the nerve mechanism that regenerates at the rate of 1 mm/day or 1 inch/month. The result of tensile test indicated that modulus values of chitosan coating variation of 1%, 1.5%, and 2% were 4.75 MPa, 4.74 MPa, and 7.67 Mpa respectively. The outcome of scanning electron microscope(SEM)showed that hollow fiberhas a diameter of 2.021-2.032 mm, which matches the diameter of peripheral nerves ranging from 1.5 to 3 mm and the membrane pore size of 31.33-39.65 μm. The result of MTT assay demonstrated that the percentage of viable fibroblastBHK-21cells was exceeding 50%, which means that the sample does not have toxic properties. The result of this study is expected to provide theoretical basis for the utilization of polyurethane-collagen coating chitosan as nervegraft for theraphy of peripheral nerve injury. The utilization is possible due to the fact that the composite exhibits biocompatible, regenerative, and easily degradable characteristics. Moreover, it could become an alternative solution to answer the need of a more affordable and easier-to-produce nerve graft, so it can be mass-produced in Indonesia.

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Desa Kare adalah salah satu desa di wilayah Kabupaten Madiun yang potensial untuk pengembangan wisata dan produk alam. Pengabdian masyarakat bertema ekowisata bertujuan (1) mengembangkan kawasan wisata berbasis partisipatif-kolaboratif, (2) meningkatkan pola kesadaran masyarakat terhadap kesehatan, (3) memberikan fasilitas kesehatan sesuai protokol kesehatan, (4) menarik minat para wisatawan melalui promosi media sosial, (5) menambah wawasan tentang cara pengolahan limbah dan produk unggulan ciri khas Desa Kare. Pelaksanaan kegiatan berlangsung dalam tiga level tahapan yaitu perencanaan kegiatan, pelaksanaan kegiatan dan tindak lanjut acara. Ekowisata dijalankan menitikberatkan pada peran aktif masyarakat setempat dan dalam kegiatan Program Pengembangan Desa Binaan (PPDB) telah dilakukan pembinaan produk dan kemasan terstandar destinasi wisata pada Unit Mikro Kecil dan Menengah (UMKM). Melalui pengmas PPDB, masyarakat setempat diberikan edukasi untuk menyiapkan masyarakat supaya sadar akan potensi alam untuk ikut menjaga dan melestarikan. Kegiatan secara berkelanjutan diharapkan untuk meningkatkan kesejahteraan serta taraf hidup masyarakat Desa Kare.