Holographic Microscopy Techniques for Multifocus Phase Imaging of Living Cells

Kemper, Björn; Langehanenberg, Patrik; Bauwens, Andreas

doi:10.1016/b978-0-12-415871-9.00006-5

Cited by 1 publication

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“…Our research falls in line with these current trends as recently digital holography has provided the numerical access to the complex optical field and digital holographic microscopy (DHM) has found numerous applications in the quantitative investigation of biological micro-objects. [3][4][5][6][7] Specifically, investigation of cells in transmission with quantitative DHM phase contrast method gained a lot of interest. 8,9 However, data captured from a single direction of observation yields the integrated phase information about optical path changes 10,11 and despite its capabilities to reconstruct images of objects at different distances, DHM cannot provide 3-D distribution of the refractive index.…”

Section: Introductionmentioning

confidence: 99%

Tomographic phase microscopy of living three-dimensional cell cultures

Kuś

Dudek

Kemper³

et al. 2014

J. Biomed. Opt.

127

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A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a "3-D cluster" as well as subcellular organelles like the nucleoli and local internal refractive index changes.

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