Tomographic phase microscopy of living three-dimensional cell cultures

Kuś, Arkadiusz; Dudek, Michał; Kemper, Björn; Kujawińska, Małgorzata; Vollmer, Angelika

doi:10.1117/1.jbo.19.4.046009

Cited by 127 publications

(65 citation statements)

References 52 publications

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“…Another means to rotate a sample is to place it inside a hollow-core optical fiber. [6,13,14] However, this solution has also some disadvantages. Once the sample is placed inside the cuvette it is not always feasible to prevent it from adhering to its inner walls.…”

Section: Problems and Solutions For Gathering Tomographic Projectionsmentioning

confidence: 97%

“…The first approach relies on capturing holographic projections during rotation of an object. [6,[12][13][14] In such case the greatest problem is the means to provide an appropriate rotation system. This may be realized using a micropipette [12] utilized to hold the object.…”

Section: Problems and Solutions For Gathering Tomographic Projectionsmentioning

confidence: 99%

“…Otherwise, applying Fourier transform phase retrieval method would be difficult to implement because the spatial carrier frequency would change with every projection direction. The key Considering the former, FC is usually mounted on a stepper motor or a motorized fiber holder (e.g., Elliot Martock MDE-235 [14] ) and FC can be placed inside a PD using syringe needles of size suitable to the FC diameter. In the other case, the MRM determines the performance of the system as the mirror may be mounted on a stepper motor [23] or a galvanometric mirror (e.g., HS-15, Nutfield Technology [24] ).…”

Section: Measurement Setups For Full and Limited Angle Projectionsmentioning

confidence: 99%

See 2 more Smart Citations

Problems and Solutions in 3-D Analysis of Phase Biological Objects by Optical Diffraction Tomography

Kujawińska

Krauze

Kuś

et al. 2014

International Journal of Optomechatronics

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Section: Problems and Solutions For Gathering Tomographic Projectionsmentioning

confidence: 97%

Section: Problems and Solutions For Gathering Tomographic Projectionsmentioning

confidence: 99%

Section: Measurement Setups For Full and Limited Angle Projectionsmentioning

confidence: 99%

See 1 more Smart Citation

Problems and Solutions in 3-D Analysis of Phase Biological Objects by Optical Diffraction Tomography

Kujawińska

Krauze

Kuś

et al. 2014

International Journal of Optomechatronics

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“…Tomography phase microscopy (TPM) [6][7][8][9][10] is an emerging technique that provides quantitative refractive index distributions and 3D structure information of biological cells. A common idea of TPM is to record a set of quantitative phase images at various illumination angles, and then to reconstruct 3D structure with these images according to the corresponding algorithm.…”

Section: Introductionmentioning

confidence: 99%

One Orthogonal Phase Microscopy Technique for Orthogonal Phase Imaging of a Cell

Liu¹,

Xu²,

Zhu³

et al. 2017

Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

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Abstract. For human beings, the morphological features of cells are closely related to human health thus research into the technique of cell imaging is very important. The optical phase imaging technique is one of the most useful methods because the cell is a phase object. More than one phase images should be needed when rebuilding the 3D sub-structure of a cell. In this paper, in order to obtain the complete 3D information of a cell under the maximum entropy tomography method, two orthogonal phase images is acquired simultaneously, so one orthogonal interference imaging system based on dual orthogonal optical path is constructed by using a lateral displacement beam splitter is proposed. It can obtain two interference images at the same time on the orthogonal direction, so that the biological cellular 3D morphology and its sub-structure can be obtained by using some phase recovery method. Compared with the traditional off-axis interferometry, the full information of the cell is omitted in this method. The simulation and experiment results show the validity and stability of the proposed method. This technique could be widely applied to phase microscopy, especially for transparent samples, such as phase imaging for biological cells and phase measurement, etc.

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“…Even more importantly, in some circumstances accurate alignment of the sample is inaccessible. This is often the case in studies of biological objects [5] and dynamic processes. Moreover, the intentional usage of the off-axis rotation scheme allows minimizing the effect of coherent noise in tomographic reconstruction [6].…”

mentioning

confidence: 98%

Time efficient method for defocus error compensation in tomographic phase microscopy

Kostencka

Kozacki

Dudek

et al. 2014

Photon. Lett. Poland

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Abstract-We present a holographic method for defocus error compensation in tomographic phase microscopy, which enables high quality reconstruction in the presence of a meaningful run-out error of the measurement system. The proposed method involves indirect determination of the sample displacement from the in-focus plane. The sought quantity is deduced from the transverse movement of the rotating sample, which can be determined with high precision using correlationbased techniques. The proposed solution features improved accuracy and reduced computation time compared to the conventional autofocusing-based approach. The validity of the concept is experimentally demonstrated by tomographic reconstruction of an optical microtip.Tomographic phase microscopy (TPM) is a laser interferometric method enabling quantitative measurement of micro-scale semi-transparent samples. Its main advantage over conventional interferometric techniques is the possibility of charactering a three-dimensional (3D) internal structure of the specimen, instead of providing integrated information about phase delays [1]. In TPM, a laser interferometric (or digital holographic) microscope is used to obtain a set of quantitative phase images of the sample collected at various illumination directions in the range 0-180º. During the measurement, the scanning of a relative angle of illumination with respect to the sample is usually achieved by rotating the object under fixed on-axis illumination. In the next step, the captured angular measurements are processed with a filtered backprojection algorithm (FBPJ) [2]. The result of the processing is the reconstruction of a 3D distribution of the refractive index inside the specimen.The basic assumption underpinning the FBPJ algorithm is that probing radiation travels through the sample along straight lines. Consequently, the 2D phase maps are interpreted by FBPJ as line integrals of the refractive index evaluated along the illumination directions. As it was proven by many researches, the straight line propagation approximation is accurate for sharp imaging conditions [3,4]. However, in the presence of a defocus error, the reconstructed 3D structure suffers from blurring and other diffraction-related deformations. In fact, all conventional microscopy systems use high NA optics to achieve high transverse resolution, which inevitably shrinks the depth of focus (DOF) to a level of a few * E-mail: j.kostencka@mchtr.pw.edu.pl microns. Thus, defocusing phase images is a key factor affecting the final quality of tomographic reconstructions.The required sharp imaging condition not only puts restrictions on the spatial extent of an investigated sample but it also implies that the sample rotation has to be done precisely around the axis passing through the centre of the sample. Otherwise, the sample displacement in the direction of the optical axis causes defocusing of the phase maps and thus erroneous tomographic reconstruction. However, precise positioning of the sample in a rotary module of the TPM system is ti...

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Tomographic phase microscopy of living three-dimensional cell cultures

Cited by 127 publications

References 52 publications

Problems and Solutions in 3-D Analysis of Phase Biological Objects by Optical Diffraction Tomography

Problems and Solutions in 3-D Analysis of Phase Biological Objects by Optical Diffraction Tomography

One Orthogonal Phase Microscopy Technique for Orthogonal Phase Imaging of a Cell

Time efficient method for defocus error compensation in tomographic phase microscopy

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