Holotomographic microscopy: A new approach to detect apoptotic cell features

Salucci, Sara; Battistelli, Michela; Burattini, Sabrina; Sbrana, Francesca; Falcieri, Elisabetta

doi:10.1002/jemt.23539

Cited by 15 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it was reported that DHT has a the potential to become an important diagnostics tool for the examination of different biological samples. 16,21,22 The obtained DHT results have brings a comparable quality to that of electron microscopy techniques, 24 which emphasizes the theoretical potential of this method in the vital imaging of nanostructures such as EVs. The aim of this study was to investigate the potential of the DHT method in imaging and the characterization of the 3D-RI distribution and morphology of single EVs to verify their heterogeneity and examine the influence of sample preparation procedures on the reconstructed RI data.…”

Section: Introductionmentioning

confidence: 62%

Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders

Zadka

Buzalewicz

Ulatowska-Jarża

et al. 2021

The American Journal of Pathology

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Introductionmentioning

confidence: 62%

Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders

Zadka

Buzalewicz

Ulatowska-Jarża

et al. 2021

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…It is known that misregulation of apoptosis can lead to cancer or autoimmunity (Goldar et al, 2015; Singh et al, 2019). On the other hand, degenerative diseases occur when apoptosis increased (Carneiro & El‐Deiry, 2020; Salucci et al, 2020). Therefore, the researcher main goal is to develop promising cancer drugs addressed to enhance apoptotic inhibitors including Bcl‐2 family proteins and other substrates.…”

Section: Discussionmentioning

confidence: 99%

“…U937, a promonocytic human myeloid leukemia cell model, has been cultured as described by Salucci et al (2020) and its behavior has been monitored with the Inverted Microscopy (IM; Eclipse TE2000‐S Nikon; objective 10×).…”

Section: Methodsmentioning

confidence: 99%

Morpho‐functional analyses reveal that changes in the chemical structure of a marine bisindole alkaloid alter the cytotoxic effect of its derivatives

Burattini

Battistelli

Verboni

et al. 2022

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

2,2-bis(6-bromo-1H-indol-3-yl) ethanamine, a marine bisindole alkaloid, showed anticancer property in several tumor cell lines thanks to the presence of a 3,3 0diindolylmethane scaffold. Here, the modifications in its chemical structure into alkaloid-like derivatives, have been evaluated, to investigate changes in its biological activities. Three derivatives have been considered and their potential apoptotic action has been evaluated through morpho-functional analyses in a human cancer cell line. Apoptosis appears strongly decreased in the derivatives without the bromine atoms (1) and in those where the bromine atoms have been substituted with fluorine atoms (2). On the contrary, the methylation of indole NH (3) does not alter the alkaloid apoptotic activity that occurs through mitochondria involvement supported by cardiolipin peroxidation and dysfunctional mitochondria presence. This manuscript highlights the alkaloid derivative cytotoxic effect, which is strictly correlated to the presence of N-methylated bisindole alkaloid and bromine atoms, conditions which assure to maintain the pro-apoptotic activity. Since molecular therapies, by targeting mitochondria pathways, have shown positive outcomes against several cancer cells, the alkaloid with bisindole methylated scaffold and the two bromine atoms can be considered a promising candidate to develop new derivatives with strong anticancer property.anticancer activity, apoptotic cell death, marine alkaloids, mitochondria damage, U937 cell line Research Highlights• 2,2-bis(6-bromo-1H-indol-3-yl) ethanamine is an alkaloid known for its anticancer properties.• Morpho-functional analyses evaluated cytotoxicity of its synthetic derivatives in tumor cells.• Anticancer properties depend on the presence of bisindole scaffold and the two bromine units.Marine bisindole alkaloids showed significant cytotoxicity, antineoplastic and antimicrobial properties (Kamel et al., 2020; Veale & Davies-Coleman, 2014). In particular, 2,2-bis(6-bromo-3-indolyl) ethanamine (BrDIMEA), extracted from the Californian tunicate Didemnum candidum and the New Caledonian sponge Orina (Salucci et al., 2018), is a strong cytotoxic agent and its anticancer

show abstract

“…Thus, confirmation of whether nLifeact-GFP induced an alteration of the chromatin structure in living cells was further attempted by holotomography, which could observe biomolecules, such as chromatin and the cell membrane, but not actin filaments, with low-energy light passing through the specimen with minimal perturbation, by obtaining the refractive index (RI) distribution 41 . As shown in Figure 5e, in the nucleus of DOXOtreated p53 knockdown cells, the holographic images showed that several straight filament-like structures were formed by the expression of nLifeact-GFP but not by that of nAC-GFP, which colocalized with nLifeact-GFP and was detected by fluorescence imaging.…”

Section: Expression Of Nlifeact-gfp Modulates the Chromatin Structure...mentioning

confidence: 99%

Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments

Torii

Sugimoto

Itoh

et al. 2023

Preprint

View full text Add to dashboard Cite

Tumor suppressor p53 plays a central role in the DNA damage response. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, it remains unclear whether p53 affects the structure of nuclear actin filaments. Here, we show that actin filament formation in the nucleus induced by the DNA-damaging agent doxorubicin (DOXO) treatment is suppressed by p53 via caspase-1 expression. In DOXO-treated cells, the nuclear actin filament formation was promoted by p53 depletion. Whilst the DOXO treatment caused an increase in caspase-1 expression, which was largely attenuated by p53 depletion, overexpression of caspase-1 reduced DOXO-induced formation of nuclear actin filaments in p53-depleted cells. By contrast, caspase-1 inhibition, together with DOXO treatment, induced nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that p53-mediated expression of caspase-1 suppresses nuclear actin filament formation. In addition, we found that expression of nLifeact-GFP, which contains the filamentous actin (F-actin)-binding peptide and nuclear localization signal (NLS), promoted the bundling of nuclear actin filaments and strongly altered the chromatin structure in DOXO-treated, p53-depleted cells. Given that the loss of p53 is associated with cancer progression, the results of this study raised the possibility that artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxicity effect of DNA-damaging agents in aggressive cancer cells.

show abstract

Holotomographic microscopy: A new approach to detect apoptotic cell features

Cited by 15 publications

References 33 publications

Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders

Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders

Morpho‐functional analyses reveal that changes in the chemical structure of a marine bisindole alkaloid alter the cytotoxic effect of its derivatives

Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments

Contact Info

Product

Resources

About