Homing in on iron homeostasis in plants

Jeong, Jeeyon; Guerinot, Mary Lou

doi:10.1016/j.tplants.2009.02.006

Cited by 269 publications

(202 citation statements)

References 77 publications

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“…Plants have evolved a delicate and complex regulation network to balance their iron content (Schmidt, 2003;Kim and Guerinot, 2007;Jeong and Guerinot, 2009). The process of iron uptake in plants involves obtaining iron from external sources.…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

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Section: Introductionmentioning

confidence: 99%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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“…For the iron uptake and transport in rice plants, several studies have been reported (Jeong and Guerinot 2009;Bashir et al, 2010;Kobayashi et al, 2010). Graminaceous plants are known to have Fe 3+ -mugineic acids (MA) complexes transporters in roots, which contribute to iron uptake from soils (Bashir et al, 2010;Kobayashi et al, 2010).…”

mentioning

confidence: 99%

Detection of Chromosomal Regions Affecting Iron Concentration in Rice Shoots Subjected to Excess Ferrous Iron Using Chromosomal Segment Substitution Lines betweenJaponicaandIndica

Fukuda¹,

Shiratsuchi

Fukushima

et al. 2012

Plant Production Science

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“…Taken together these data provide support to the idea that lack, or diminished, TaNAM activity generates a functional stay-green phenotype, and that this is accompanied by preservation of full chloroplast structure. Degradation of chloroplasts is considered to be an important source of nitrogen (Martinez et al, 2008;Gregersen et al, 2008), iron (Jeong and Guerinot, 2009) and other nutrients, like zinc (Broadley et al, 2007). In this context, the preservation of chloroplast structure observed here would diminish the pool of nutrients available for export, thus contributing to reduce the export of those nutrients.…”

Section: Discussionmentioning

confidence: 81%

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

Checovich

Galatro

Moriconi

et al. 2016

Plant Physiology and Biochemistry

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SummaryTaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity. 3 Highlights•TaNAM-RNAi stay-green phenotype is coupled with maintenance of chloroplast structure.•That stay-green phenotype is associated with a high enzymatic antioxidant activity.•Chloroplast dismantling correlates with decay of iron and protein content in leaves.

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Homing in on iron homeostasis in plants

Cited by 269 publications

References 77 publications

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

Detection of Chromosomal Regions Affecting Iron Concentration in Rice Shoots Subjected to Excess Ferrous Iron Using Chromosomal Segment Substitution Lines betweenJaponicaandIndica

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

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