Homogeneous chicken heart mitochondrial creatine kinase purified by dye-ligand and transition-state analog-affinity chromatography

Brooks, Stephen P. J.; Bennett, Vickie D.; Suelter, Clarence H.

doi:10.1016/0003-2697(87)90385-x

Cited by 14 publications

(1 citation statement)

References 35 publications

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“…Changes in activity reflect pathological alterations in specific human organs. Creatine kinase has also been studied in other species including dogs, chickens, rabbits and mice (Brooks, Bennett & Suelter 1987). It is now widely accepted that serum enzyme activity reflects the health condition of fish, and their use in diagnosis has been recommended by a number of authors (Hlavova 1989).…”

Section: Introductionmentioning

confidence: 99%

Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing

Williams

Stewart

Timpson

1996

Journal of Fish Diseases

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Serum samples were obtained from rainbow trout, Oncorhynchus mykiss (Walbaum), from a commercial fish farm. Creatine kinase (CK; EC 2.7.3.2) isoenzymes and sub-bands (isoforms) were studied using agarose gel electrophoresis and polyacrylamide gel isoelectric focusing. Creatine kinase isoenzymes and isoforms (sub-bands) were found to display disparate electrophoretic mobility and isoelectric points, respectively, when compared with CK patterns of human origin. The CK-BB isoenzyme, rarely detected in human serum, was detected in all trout samples assayed. Up to eight CKMM isoforms were detected by the polyacrylamide gel IEF method described. Correspondence

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Section: Introductionmentioning

confidence: 99%

Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing

Williams

Stewart

Timpson

1996

Journal of Fish Diseases

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Affinity Chromatography

Batista‐Viera¹,

Janson²,

Carlsson³

2011

Methods of Biochemical Analysis

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Two types of resin-conjugated Tamiflu analogs were synthesized by modifying our original synthetic route of oseltamivir phosphate (Tamiflu). The prepared resins bound to influenza virus neuraminidase, the main target of Tamiflu. The resins will be useful for isolating and identifying presumed endogenous vertebrate proteins that interact with Tamiflu, which might relate to the rarely observed abnormal behavior exhibited by some influenza patients treated with Tamiflu.

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Discrete subcellular localization of a cytoplasmic and a mitochondrial isozyme of creatine kinase in intestinal epithelial cells

Keller

Gordon

1991

Cell Motil. Cytoskeleton

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Two isozymes of creatine kinase have been purified differentially from mitochondrial and cytoplasmic subfractions of intestinal epithelial cells. These intestinal epithelial cell creatine kinases were indistinguishable from the cytoplasmic (B-CK) and mitochondria1 (Mi-CK) creatine kinase isozymes of brain when compared by SDS-PAGE, cellulose polyacetate electrophoresis, and peptide mapping. In intestinal epithelial cells, immunolocalization of the Mi-CK isozyme indicates that it is associated with long, thin mitochondria, which are excluded from the brush border at the apical end of each cell. In contrast, immunolocalization of the B-CK isozyme indicates that it is concentrated distinctly in the brush border terminal web domain. Although absent from the microvilli, B-CK also is distributed diffusely throughout the cytoplasm. Terminal web localization of B-CK was maintained in glycerol-permeabilized cells and in isolated brush borders, indicating that B-CK binds to the brush border structure. The abundance and localization of the mitochondrial and cytoplasmic creatine kinase isozymes suggest that they are part of a system that temporally and/or spatially buffers dynamic energy requirements of intestinal epithelial cells.

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Homogeneous chicken heart mitochondrial creatine kinase purified by dye-ligand and transition-state analog-affinity chromatography

Cited by 14 publications

References 35 publications

Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing

Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing

Affinity Chromatography

Discrete subcellular localization of a cytoplasmic and a mitochondrial isozyme of creatine kinase in intestinal epithelial cells

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