Homologs of LEAFY and UNUSUAL FLORAL ORGANS Promote the Transition From Inflorescence to Floral Meristem Identity in the Cymose Aquilegia coerulea

Sharma, Bharti; Meaders, Clara L.; Wolfe, Damien; Holappa, Lynn D.; Walcher-Chevillet, Cristina L

doi:10.3389/fpls.2019.01218

Cited by 8 publications

(10 citation statements)

References 44 publications

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“…By contrast, the five periphery hub genes with the largest number of edges are (Fig. 6 ): AqVRN1 ( Aqcoe2G107800 , homologous to VERNALIZATION1 , VRN1 , in A. thaliana ); AqHCT ( Aqcoe7G354200 , codes for a hydroxycinnamoyl-Coenzyme A shikimate/quinate hydroxycinnamoyltransferase, HCT); AqUFO1 ( Aqcoe1G161900 ; UNUSUAL FLORAL ORGANS1 12 ), AqSAP; and AqEPFL4 ( Aqcoe2G352600 , homologous to EPIDERMAL PATTERNING FACTOR-LIKE4 , EPFL4 , in A. thaliana ). The functional implications of some of these loci are unclear.…”

Section: Resultsmentioning

confidence: 99%

“…Homologs of UFO encode F-box proteins that generally function with LEAFY (LFY) homologs to promote FM identity and activate B-class gene expression across diverse dicot model systems [54][55][56][57][58] . In Aquilegia, the closely related paralogs AqUFO1 and AqUFO2 promote the transition from inflorescence to FM identity in conjunction with AqLFY, although there is no clear role in B gene activation 12 . In addition to the expected contribution to FM identity, the AqUFO1/2 paralogs also appear to promote the initiation of the stamen whorls, as whorl number dramatically decreases in silenced flowers from the usual 7-8 whorls to only 4-5.…”

Section: Differential Expression Analysis Between Developmental Stagesmentioning

confidence: 99%

“…1 ). Using a candidate gene approach, previous studies have revealed the sub-functionalization of the B-class organ identity genes 10 – 12 and that the JAGGED homolog is crucial for initiation of the floral organ primordia 13 , but genome scale studies to date have focused on late stage floral organ development 14 – 16 .

Figure 1 A. coerulea and the floral developmental stages used for RNA-seq.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Min

Kramer

2020

Sci Rep

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The earliest phases of floral development include a number of crucial processes that lay the foundation for the subsequent morphogenesis of floral organs and success in reproduction. Currently, key transcriptional changes during this developmental window have been characterized in the model species Arabidopsis thaliana, but little is known about how transcriptional dynamics change over the course of these developmental processes in other plant systems. Here, we have conducted the first in-depth transcriptome profiling of early floral development in Aquilegia at four finely dissected developmental stages, with eight biological replicates per stage. Using differential gene expression analysis and weighted gene co-expression network analysis, we identified both crucial genes whose expression changes mark the transitions between developmental stages and hub genes in co-expression modules. Our results support the potential functional conservation of key genes in early floral development that have been identified in other systems, but also reveal a number of previously unknown or overlooked loci that are worthy of further investigation. In addition, our results highlight not only the dynamics of transcriptional regulation during early floral development, but also the potential involvement of the complex, essential networks of small RNA and post-translational regulation to these developmental stages.

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Section: Resultsmentioning

confidence: 99%

Section: Differential Expression Analysis Between Developmental Stagesmentioning

confidence: 99%

Figure 1 A. coerulea and the floral developmental stages used for RNA-seq.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Min

Kramer

2020

Sci Rep

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“…Total RNA was extracted from leaves using the TIANGEN RNA kit, and cDNA was obtained using the TaKaRa Reverse Transcription System (RR047A) (TaKaRa, Dalian, China) [58].…”

Section: Molecular Cloning Of Jmlfy Full-lengthmentioning

confidence: 99%

Molecular Cloning, Expression Analysis and Subcellular Localization of LEAFY in <i>Juglans mandshurica</i> Maxim

Liu¹,

Lu²,

Song³

et al. 2022

Pol. J. Environ. Stud.

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Juglans mandshurica is a precious timber and economic tree species in Northeast China. Because of the reproductive characteristics of this species, such as long childhood and dioecious, the flowering period of male and female flowers is different, which directly affects the sexual reproduction process such as flowering and pollination. At present, the molecular mechanism of dioecious flowering of J. mandshurica is not clear. In this study, the cDNA sequence of a crucial gene involved in plant flowering, LEAFY (LFY) was cloned from J. mandshurica by homologous cloning, and the expression in different organs at various developmental stages, and the subcellular localization of the gene were analyzed. LFY in J. mandshurica was found to possess high homology (over 79%), and a similar structure and function to LFY in other species, and was cloned and named JmLFY (genBank: KX364241). The results of fluorescence quantitative real-time PCR analysis revealed that JmLFY was expressed in different organs, and that expression increased significantly before the early development stage of staminate inflorescence, the anther formation and pollen dispersal stage, pistillate inflorescence sprouting and flower development stage, and the pollination and late stage of zygotic embryo development. Expression of the green fluorescent protein fusion JmLFY gene in onion epidermal cells was localized to the nucleus and cytoplasm. Our results indicate that the JmLFY gene is the key transcription factor involved in flower formation and development in J. mandshurica. These findings provide information that may help to overcome the bottleneck caused by the long juvenile period and asynchronous female and male flowers of the species in the future.

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“…In Arabidopsis, the Atufo mutation causes a series of floral abnormalities, such as in the number of sepals, flower size, and so on [59]. Similarly, the ufo mutation in Cymose Aquilegia coerulea results in small flowers, increased sepals, and decreased petals and stamens [61]. In rice, the functions of class B genesOsMADS4 and OsMADS16 are largely conservative, being regulated by the F-box geneDDF1 [62].…”

Section: Flower and Fruit Developmentmentioning

confidence: 99%

The Biological Function and Roles in Phytohormone Signaling of the F-Box Protein in Plants

Yao

et al. 2021

Agronomy

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The ubiquitin–proteasome pathway (UPP) is an important protein degradation pathway that can participate in the regulation of the physiological process of organisms by specifically removing abnormal peptides and degrading cell regulators. UPP mainly involves three enzymes, among which the E3 ubiquitin ligase function is central to UPP. E3 ubiquitin ligases can recruit substrate protein for ubiquitination, and they have various forms. Among them, the Skp1–Cul1–F-box (SCF) complex is the most representative member of the cullin RING ubiquitin ligases type in RING-domain E3 ligases, being mainly composed of Cullin 1, Skp1, Rbx1, and F-box proteins. The F-box protein is the key component for SCF to perform specific functions. The F-box protein is one of the largest protein families in plants, and its family members are involved in the regulation of many key physiological processes, such as growth and development of plants and the response to external stimuli. Herein, we briefly review the structure, classification, function, and hormone signaling pathways of F-box proteins.

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Homologs of LEAFY and UNUSUAL FLORAL ORGANS Promote the Transition From Inflorescence to Floral Meristem Identity in the Cymose Aquilegia coerulea

Cited by 8 publications

References 44 publications

Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Transcriptome profiling and weighted gene co-expression network analysis of early floral development in Aquilegia coerulea

Molecular Cloning, Expression Analysis and Subcellular Localization of LEAFY in <i>Juglans mandshurica</i> Maxim

The Biological Function and Roles in Phytohormone Signaling of the F-Box Protein in Plants

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