Homology among <i>Treponema denticola</i> plasmids

Reedy, E.; Simonson, Lloyd G.; Zablen, L.

doi:10.1111/j.1399-302x.1994.tb00076.x

Cited by 4 publications

(6 citation statements)

References 15 publications

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“…2. Similarity in the profile of the same digestion with the plasmids from U2b (lanes 2, 4, 6, 8, 10, 12, 14, 16, 18) and U9c (lanes3,5,7,9, 11, 13, 15, 17, 19). Left to right: lane 1: supercoiled DNA ladder of 16 kb; lanes 2, 3: uncut plasmid DNA; lanes 4, 5: Pvu II; lanes 6, 7:…”

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confidence: 78%

“…The 4.2-kb plasmid is novel and is larger than the pTDl described previously in T. dentieola by Ivic (3) and by Reedy (7). It is present in several strains of Treponema isolated from clinical sources, namely, strains U2a, U2b, U9b and U9c.…”

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confidence: 82%

“…Bacillus species) (6). The isolation of two further plasmids (7), both from T. dentieola, suggests that this species in vivo can acquire and maintain transferrable plasmids, a process that may contribute to their virulence in pedodontal disease. In a previous study, we investigated the presence of plasmids in clinical isolates of other Treponema species and determined whether such plasmids were similar to those isolated from T. dentieola (1).…”

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confidence: 99%

“…Oral anaerobic spirochetes have been implicated in the initiation and progression of periodontal disease (9). Plasmids have recently been descdbed to be present among them, specifically, in Treponema dentieola, a spirochete species commonly found in subgingival plaque (3,7). The plasmid sizes were reported to be in the range of 2.0 -2.7 kb.…”

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confidence: 99%

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Characterization of a 4.2‐kb plasmid isolated from periodontopathic spirochetes

Chan

Klitorinos

Gharbia

et al. 1996

Oral Microbiology and Immunology

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Oral anaerobic treponemes are assoicated with active periodontal disease and may comprise up to 57% of the microbiota in periodontal pockets. Four treponeme strains (designated U2a, U2b, U9b, and U9c) isolated from clincial cases were found to harbor a new 4.2-kb plasmid when plasmid DNA was extracted and purified employing the Qiagen Plasmid Kit. This plasmid differs from the smaller plasmids (2.0-, 2.6-, and 2.7-Kb) reported previously by others in Treponema denticola. The newly discovered 4.2-kb plasmid was found to be the same in all four treponeme strains by restriction endonuclease analysis. It is a circular plasmid since restriction with PstI, Pvu II, Sma I, Xma I, Ava 1 or Bam HI produced a single band of the same size. Bacterial strain U2b was shown to be Treponema socranskii and U9c to be T. denticola. The plasmid is designated "pTS1". The presence of the same plasmid in different species of the treponemes isolated from the same patient suggests the possibility of a naturally occurring genetic transfer system within the oral spirochetes or their ability to take up and maintain mobilizable plasmids.

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confidence: 78%

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confidence: 82%

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confidence: 99%

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confidence: 99%

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Characterization of a 4.2‐kb plasmid isolated from periodontopathic spirochetes

Chan

Klitorinos

Gharbia

et al. 1996

Oral Microbiology and Immunology

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“…Monolayers of human and canine epithelial cells incubated with DMEM without tréponèmes, and treated with monoclonal antibodies in the immunocytochemical assay, did not show artifactual or unusual deposits of chromogen (data not shown). T. denticola ATCC #35404 (C) 23 9 0 32 22 10 3 35…”

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confidence: 99%

Differential Attachment of Oral Treponemes to Monolayers of Epithelial Cells

Carranza

Riviere²,

Smith³

et al. 1997

Journal of Periodontology

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This in vitro study describes the attachment properties of several oral treponemes to monolayers of epithelial cells and the effect of epithelial cell confluence on treponeme attachment. Four serotypes of Treponema denticola, Treponema scoliodontum, three subspecies of Treponema socranskii and Treponema vincentii were tested with monolayers of epithelial cells of human and canine origin. Attachment of oral treponemes were compared to attachment by T. pallidum subsp. pallidum, and by the nonpathogen Treponema phagedenis. Results indicated that different serotypes of T. denticola had similar abilities to attach to epithelial cells. However, subspecies of T. socranskii differed in their ability to attach to epithelial cells. The proportion of epithelial cells susceptible to attachment by oral spirochetes was strongly related to the confluence level of the monolayer. In contrast, T. pallidum attached equally well to both epithelial cell lines at all confluence levels. T. phagedenis attached to <1% of all epithelial cells. In general, attachment of oral treponemes to canine cells was lower than to human cells, suggesting species‐specificity for adherence. Attachment of oral treponemes to epithelial cells may promote colonization of the periodontal pocket, as well as retention of treponeme colonies within plaque. The preference of oral treponemes to attach to cells of low confluence fields may translate in vivo to an increased ability to attach to cells which are actively dividing. Such cells are found in areas of repair, a common status within inflamed periodontal pockets. Furthermore, attachment of oral treponemes to epithelial cell barriers may promote or potentiate cytopathic processes. J Periodontol 1997;68:1010–1018.

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