Homotypic Clusters of Transcription Factor Binding Sites in the First Large Intron of AGL24 MADS-Box Transcription Factor Are Recruited in the Enhancement of Floral Expression

Hussain, Tajammul; Rehman, Nazia; Inam, Safeena; Ajmal, Wajya; Afroz, Amber; Muhammad, Aish; Zafar, Yusuf; Ali, Ghulam Muhammad; Khan, Muhammad Ramzan

doi:10.1007/s11105-019-01136-7

Cited by 3 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, we found that the expression levels of WRKY, MYB, bZIP and other transcription factors changed significantly under MeJA. Most of the transcription factors function in a mutually binding manner, forming a vast network of plant gene regulation [ 80 , 81 , 82 ]. Yang et al [ 70 ] showed that MYB21 and MYC2 interact to regulate the expression of terpenoid synthase genes in flowers of Freesia hybrida , which affects the biosynthesis of linalool.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

Shi

Cui

Zhang

et al. 2023

CIMB

View full text Add to dashboard Cite

Background: Methyl jasmonate has an important effect on the synthesis of plant secondary metabolites. Schizonepeta tenuifolia Briq. has a wide range of pharmacological effects and the secondary metabolites are dominated by monoterpenes (pulegone, menthone). Objective: It is essential to determine the changes in secondary metabolites in S. tenuifolia under methyl jasmonate treatment and to probe the molecular mechanism. This can improve the accumulation of secondary metabolites in the medicinal plant S. tenuifolia and enrich the information gene expression at different MeJA levels, which can help to elucidate the molecular mechanism of monoterpenoid synthesis in S. tenuifolia. Methods: In this study, we determined the changes in the content of monoterpenoids in S. tenuifolia under methyl jasmonate treatment. Meanwhile, we established a transcriptome database of S. tenuifolia under methyl jasmonate level using high-throughput sequencing. Results: A certain concentration of MeJA promoted the accumulation of monoterpenoids in S. tenuifolia. The transcriptome database of S. tenuifolia leaves under 0, 50, 100 and 250 μM MeJA treatment was established. We generated 88,373 unigenes with an N50 length of 2678 bp, of which 50,843 (57.53%) can be annotated in at least one database. Compared with the CK (0 μM) group, 12,557 (50 μM), 15,409 (100 μM) and 13,286 (250 μM) differentially expressed genes were identified. GO and KEGG enrichment analysis revealed that JA signal transduction and monoterpenoid synthesis were the two most significant enrichment pathways. The expression levels of related DEGs involved in JA signaling and monoterpenoid synthesis were significantly up-regulated by MeJA. In addition, our phenotypic and differentially expressed gene association analysis revealed that monoterpenoid biosynthesis in S. tenuifolia was more associated with genes involved in plant trichome branching, phytohormone signaling and transcriptional regulation. Conclusions: This study confirmed that methyl jasmonate significantly promoted monoterpenoid biosynthesis in S. tenuifolia. A large number of genes responding to methyl jasmonate were associated with JA signaling and monoterpenoid biosynthesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

Shi

Cui

Zhang

et al. 2023

CIMB

View full text Add to dashboard Cite

show abstract

“…Genome-wide analyses indicate that most in vivo functional TFBSs are located in the proximal promoter region [ 20 , 21 ]. These sites form clusters, thus improving interactions of corresponding transcription factors (TFs) to ensure a better execution of their regulatory functions [ 22 ]. An essential functional linkage exists between TFs and RNA polymerase II, acting as a large, conformationally flexible multiprotein complex known as a Mediator [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Comprehensive in Silico Characterisation of a New 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase 4 (HMGR4) Gene Promoter from Salvia miltiorrhiza: Comparative Analyses of Plant HMGR Promoters

Majewska

Kuźma

Szymczyk

2022

Plants

View full text Add to dashboard Cite

Salvia miltiorrhiza synthesises tanshinones with multidirectional therapeutic effects. These compounds have a complex biosynthetic pathway, whose first rate limiting enzyme is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR). In the present study, a new 1646 bp fragment of the S. miltiorrhiza HMGR4 gene consisting of a promoter, 5′ untranslated region and part of a coding sequence was isolated and characterised in silico using bioinformatics tools. The results indicate the presence of a TATA box, tandem repeat and pyrimidine-rich sequence, and the absence of CpG islands. The sequence was rich in motifs recognised by specific transcription factors sensitive mainly to light, salicylic acid, bacterial infection and auxins; it also demonstrated many binding sites for microRNAs. Moreover, our results suggest that HMGR4 expression is possibly regulated during flowering, embryogenesis, organogenesis and the circadian rhythm. The obtained data were verified by comparison with microarray co-expression results obtained for Arabidopsis thaliana. Alignment of the isolated HMGR4 sequence with other plant HMGRs indicated the presence of many common binding sites for transcription factors, including conserved ones. Our findings provide valuable information for understanding the mechanisms that direct transcription of the S. miltiorrhiza HMGR4 gene.

show abstract

Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

et al. 2019

View full text Add to dashboard Cite

Homotypic Clusters of Transcription Factor Binding Sites in the First Large Intron of AGL24 MADS-Box Transcription Factor Are Recruited in the Enhancement of Floral Expression

Cited by 3 publications

References 83 publications

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

Isolation and Comprehensive in Silico Characterisation of a New 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase 4 (HMGR4) Gene Promoter from Salvia miltiorrhiza: Comparative Analyses of Plant HMGR Promoters

Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

Contact Info

Product

Resources

About