Honey is cytotoxic towards prostate cancer cells but interacts with the MTT reagent: Considerations for the choice of cell viability assay

Abel, Sean; Baird, Sarah K.

doi:10.1016/j.foodchem.2017.08.083

Cited by 72 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Morphological changes during cell death are essential criteria in apoptosis that can be measured by AO/EB staining (Abel & Sarah, ). Collectively, if the cells were undergoing a specific pattern of cell death by AO/EB staining, cytological changes would have been observed.…”

Section: Resultsmentioning

confidence: 99%

Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer

Moideen

Kavitha

Kandhasamy

et al. 2019

J Food Process Engineering

View full text Add to dashboard Cite

This research is aimed at the preparation of biopolymeric skimmed milk powder (SMP) and pectin coated dual layer solid lipid nanoparticles loaded with soluble curcumin (CMN) to enhance the cytotoxicity of the drug delivered to the colon for colorectal cancer (CRC). The curcumin (C‐) and curcumin solid dispersion (C‐SD) (soluble curcumin) loaded solid lipid nanoparticle (SLN) (C‐SD‐SLN) are prepared by hot homogenization method and characterized its physicochemical properties. The surface of the C‐SD‐SLNs was coated with natural biopolymers of pectin and skimmed milk powder (SMP), called cetyl palmitate (CP)‐SD‐SLN. The resultant nanoparticles showed significantly high drug loading, better stability, and slower release profile (92.1%) in various GI conditions up to 72 H. zebra fish toxicity studies of CP‐SD‐SLN confirm its in vivo safety, and the result from in vitro cytotoxicity showed that 50% inhibition (26 μM/ml) is achieved with an amount of CP‐SD‐SLN, which was considerably lesser than free CMN. The result from flow cytometry analysis confirmed the cell cycle arrest in the G2/M phase (49.11%), and western blot analysis was performed to understand its molecular level apoptosis in Caco‐2 cells. The profound efficiency of SMP and pectin coated CP‐SD‐SLN is a promising preparation that indicated its potential application for CRC treatment without toxicity. Practical application The pharma and biotech industry is seeking techniques to increase potential and reduce the toxicity of chemotherapeutic agents. The solid lipid nanoparticle (SLN) has fascinated significant attention in anticancer drug delivery generally because of the targeted delivery to the particular site and devoid of other tissues. The present investigation provides the efficiency of skimmed milk powder (SMP)‐coated lipid shell contains soluble curcumin to release the drug at colon and improve the metabolism and therefore increase the potential activity several fold than native curcumin. The results show that SMP and pectin coated SLN is a potential carrier for delivery to the colon and thus improve anticancer potential of human adenocarcinoma (Caco‐2) cells of curcumin. These results will contribute in designing SLN techniques for optimizing the wide‐ranging quality of natural curcumin.

show abstract

Section: Resultsmentioning

confidence: 99%

Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer

Moideen

Kavitha

Kandhasamy

et al. 2019

J Food Process Engineering

View full text Add to dashboard Cite

show abstract

“…This assay is a cost effective and rapid method for investigating cell viability or proliferation . The reduction reaction of the tetrazolium compound to formazan by cellular enzymes, including mitochondrial NAD(P)H‐dependent oxidoreductases and dehydrogenases, is lowered in a population of cells exposed to a toxic compound . To investigate that PMCTi suppressed cell viability, HepG2, KKU‐005, and HuH‐7 cells were used.…”

Section: Resultsmentioning

confidence: 99%

Suppressed de novo lipogenesis by plasma membrane citrate transporter inhibitor promotes apoptosis in HepG2 cells

et al. 2018

View full text Add to dashboard Cite

Suppression of the expression or activities of enzymes that are involved in the synthesis of de novo lipogenesis ( DNL ) in cancer cells triggers cell death via apoptosis. The plasma membrane citrate transporter ( PMCT ) is the initial step that translocates citrate from blood circulation into the cytoplasm for de novo long‐chain fatty acids synthesis. This study investigated the antitumor effect of the PMCT inhibitor ( PMCT i) in inducing apoptosis by inhibiting the DNL pathway in HepG2 cells. The present findings showed that PMCT i reduced cell viability and enhanced apoptosis through decreased intracellular citrate levels, which consequently caused inhibition of fatty acid and triacylglycerol productions. Thus, as a result of the reduction in fatty acid synthesis, the activity of carnitine palmitoyl transferase‐1 ( CPT ‐1) was suppressed. Decreased CPT ‐1 activity also facilitated the disruption of mitochondrial membrane potential (ΔΨm) leading to stimulation of apoptosis. Surprisingly, primary human hepatocytes were not affected by PMCT i. Increased caspase‐8 activity as a consequence of reduction in fatty acid synthesis was also found to cause disruption of ΔΨm. In addition, apoptosis induction by PMCT i was associated with an enhanced reactive oxygen species generation. Taken together, we suggest that inhibition of the DNL pathway following reduction in citrate levels is an important regulator of apoptosis in HepG2 cells via suppression of CPT ‐1 activity. Thus, targeting the DNL pathway mediating CPT ‐1 activity by PMCT i may be a selective potential anticancer therapy.

show abstract

“…On analyzing with MTT assay results, TM was more hepatoprotective than T. But in flow cytometry, the T was more hepatoprotective than TM. This may be due to the fact that the MTT assay was confounded by the reduction of MTT reagent by honey's reducing sugars and phenolic compounds [19] Triphala has been used since ancient times to treat wide range of disease. It is extensively used to treat hepatic disorders and even possesses anti diabetic, anti-hypertensive, anti dyslipidaemic, anti oxidant activities.…”

Section: Result:-mentioning

confidence: 99%

Hepatoprotective Effect of Triphala and Its Combinations - In Hepg2 Cell Lines.

VM¹

2019

IJAR

View full text Add to dashboard Cite

Introduction:From ancient period onwards Triphala and its combinations were used in liver disorders in Ayurveda, but its mechanism of action are not still evaluated. Triphala is the combination in equi-proportion 1:1:1, of dried fruits of Amalaki (Phyllanthus emblica Linn), Bibhitaki (Terminalia bellerica Roxb) and Haritaki (Terminalia chebula Retz). The present study evaluated the hepatoprotective effect of methanolic extract of Triphala (T) and its different combinations such as Triphala with honey [TM], Triphala with Pippali (dried fruit of Piper longum Linn) [TP] and Triphala with Yashtimadhu (rhizhomes of Glycyrrhiza glabra Linn) [TY] in HepG 2 cell lines using MTT assay against acetaminophen induced hepato toxicity. Flow cytometry was used to count the viable cells. Methodology:HepG 2 (Human hepatic carcinoma) cell line was purchased from NCCS Pune and maintained in Dulbecco's modified eagle's media. Then T, TM, TP and TY were added in to acetaminophen induced hepatotoxicity cells at five concentrations; 6.25µg/ml, 12.5 µg/ml, 25 µg/ml, 50 µg/ml and 100 µg/ml using MTT assay [(3-(4,5 dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay]; untreated cells and acetaminophen treated cells kept as controls. Results:The acetaminophen treated cells showed 34.15% viability in MTT assay. The T, TM, TP and TY treated groups showed increase in the percentage cell viability at 6.25µg/ml, 12.5 µg/ml, 25 µg/ml, 50 µg/ml and 100 µg/ml concentrations and the result were significant (p<0.05), when compared to acetaminophen treated group. In flow cytometry total apoptosis was 28.88% for T,39.04% for TM ,42.04% for TP and 44.12% for TY. Conclusion:The results validate that Ayurveda combination T has more hepato protective effect in HepG 2 cell line; in the increasing order TY< TP < TM < T.

show abstract

Honey is cytotoxic towards prostate cancer cells but interacts with the MTT reagent: Considerations for the choice of cell viability assay

Cited by 72 publications

References 34 publications

Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer

Skimmed milk powder and pectin decorated solid lipid nanoparticle containing soluble curcumin used for the treatment of colorectal cancer

Suppressed de novo lipogenesis by plasma membrane citrate transporter inhibitor promotes apoptosis in HepG2 cells

Hepatoprotective Effect of Triphala and Its Combinations - In Hepg2 Cell Lines.

Contact Info

Product

Resources

About