“…The ab-normalH 2 O 2 level in tissues can potentially induce various types of damage to cellular compartments, consequently causing aging, atherosclerosis, cancer,a nd renal diseases. Therefore, it is extremelye ssential to establish reliable methods to detectc holesterol with high sensitivitya nd selectivity.T od ate, various methods such as electrochemistry sensors, [6][7][8][9][10][11][12] fluorometric analysis, [13][14][15] chromatographic analysis, [16][17][18] molecular imprinting techniques, [19] surface plasma resonance, [20] fieldeffect-transistor sensors, [21] chemiluminescence, [22] and colorimetric methods [23,24] have been reported to detect cholesterol and H 2 O 2 .A mongt hem, the colorimetric methodi sp opular becauseo fi ts rapid ands imple operation as well as good reproducibility.I na ddition, the color change during colorimetric detection could be directly observed by the eyes insteado fb y using expensive instruments. [25,26] It is well known that natural horseradish peroxidase (HRP) can be used for colorimetricd etection.…”