“HOOF-Print” genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Almendra, Cláudia; Silva, Teresa Luísa; Beja‐Pereira, Albano; Ferreira, Ana Cristina; Ferrão-Beck, Lénia; Sá, Maria Inácia Corrêa de; Bricker, Betsy J.; Luikart, Gordon

doi:10.1016/j.meegid.2008.10.007

Cited by 7 publications

(6 citation statements)

References 14 publications

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“…A hypervariable or unstable maker may indicate the wrong source of infection, so it would not be helpful as an epidemiological marker. Bruce 16 and Hoof-3 have a maximum of six and ten allelic types, and the SDI values were 0.758 (Table 1), which is similar to the case with Brucella species previously reported [1,2,24]. Above all, unlike Bruce 9, Bruce 16 and Hoof-3, which had the highest DI values, did not vary according to the isolates from each farm (Table 3).…”

Section: Discussionsupporting

confidence: 82%

Genetic Comparison of Brucella canis Isolates by the MLVA Assay in South Korea

Kang

Heo

Cho

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The multiple-locus VNTR analysis (MLVA) assay is a method frequently employed as a molecular epidemiological tool for Brucella genetic fingerprinting. The purpose of this study was to assess the genotyping of 77 B. canis isolates from 14 different dog breeding farms in Korea by the MLVA assay and to compare the epidemiological relationships between the Korean isolates and foreign ones. Simpson's diversity index for 17 loci showed a range from 0 to 0.846 in 77 B. canis isolates. B. canis isolates in Korea were observed to have high genetic diversity at the most variable loci and were divided into 30 distinct genotypes by phylogenetic analysis. Some B. canis isolates were closely related to previously typed isolates in other countries. The MLVA assay can be helpful to analyze the epidemiological correlation of B. canis isolates in domestic pet animals and to track the geographic origin by comparing the genetic patterns with foreign isolates. Therefore, the MLVA assay will be useful as a tool for control and preventive measures of canine brucellosis.

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Section: Discussionsupporting

confidence: 82%

Genetic Comparison of Brucella canis Isolates by the MLVA Assay in South Korea

Kang

Heo

Cho

et al. 2011

The Journal of Veterinary Medical Science

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“…With just half of the markers required for MLVA-16 typing, HOOF-printing showed a high degree of intraspecific diversity (6,32); it is therefore a powerful tool for typing of B. melitensis strains collected from single countries (2,30). In the present population, the allelic richness was especially marked in TRs 1, 4, 5, and 7 (13 to 16 alleles).…”

Section: Discussionmentioning

confidence: 70%

Epidemiological and Phylogenetic Analysis of Spanish HumanBrucella melitensisStrains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, andrpoBTyping

et al. 2010

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The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase ␤ subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (

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“…The Brucella 2009 genotyping database available at http://mlva.u-psud.fr/ and based upon the data provided in Additional file 1 can be used for this purpose. Figure 4 shows the global population structure of the nine species currently constituting the Brucella genus, as can be revealed by MLVA-16 typing using this dataset (the extended data set provided here may provide new opportunities to evaluate additional methods for Brucella MLVA data clustering recently proposed [34]).…”

Section: Discussionmentioning

confidence: 99%

MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

et al. 2009

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BackgroundSince 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used.Results294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates.ConclusionThe clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species.

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“HOOF-Print” genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Cited by 7 publications

References 14 publications

Genetic Comparison of Brucella canis Isolates by the MLVA Assay in South Korea

Genetic Comparison of Brucella canis Isolates by the MLVA Assay in South Korea

Epidemiological and Phylogenetic Analysis of Spanish HumanBrucella melitensisStrains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, andrpoBTyping

MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

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