Hormonal control of accessory sex organ fibromuscular stroma

Mariotti, Angelo; Mawhinney, Michael G.

doi:10.1002/pros.2990020407

Cited by 20 publications

(13 citation statements)

References 15 publications

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“…act. >31 TBq/mmol) and [l,2,6,7-3H] A linear relationship exists between guinea-pig age and body weight during the first 12 to 14 weeks of age (Rigaudière, Pelardy, Robert & Delost, 1976;Mariotti & Mawhinney, 1981). This relationship has previously been established for the strain of guineapigs used in the present study (Institute of Medical and Veterinary Science, 1981).…”

Section: Steroids and Chemicalssupporting

confidence: 65%

Effects of ageing and hormonal manipulations on the level of oestrogen receptors in the guinea-pig prostate

Tilley¹,

Horsfall²,

McGee³

et al. 1987

Journal of Endocrinology

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Cytosolic oestrogen receptor levels in guinea-pig prostate tissue were found to decrease with increasing age, irrespective of whether the binding was expressed relative to cytosolic protein or cellular DNA. This decrease in oestrogen receptor levels was also observed using enriched fibromuscular stromal tissue prepared by mechanical fractionation of the prostate. The most pronounced change in cytosolic oestrogen receptor levels (from 133 to 35 fmol/mg protein) occurred at the onset of puberty. The pubertal decrease in receptor levels could not be attributed to an increase in the level of proteolytic activity in prostatic cytosol fractions derived from mature animals, a change in the affinity of the receptor for oestradiol or an increase in oestrogen receptor levels in salt-extracted nuclear fractions. Administration of tamoxifen (1 mg/day) to intact guinea-pigs throughout the transpubertal growth phase did not influence the age-related decrease in cytosolic and nuclear oestrogen receptor levels. In contrast, the decrease in oestrogen receptor levels was prevented by castration. Administration of dihydrotestosterone (DHT; 1 mg/day) to intact prepubertal animals for 4 days before study resulted in diminished cytosolic oestrogen receptor levels; this effect of DHT was blocked by the non-steroidal antiandrogen flutamide (2 mg/day). Furthermore, elimination of testicular hormones by castration during the late-pubertal growth phase resulted in a greater than twofold increase in prostatic oestrogen receptor levels. Collectively, these observations suggest an age-related decrease in oestrogen receptor levels in the guinea-pig prostate which, in part, may be due to increased testicular function at puberty.

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Section: Steroids and Chemicalssupporting

confidence: 65%

Effects of ageing and hormonal manipulations on the level of oestrogen receptors in the guinea-pig prostate

Tilley¹,

Horsfall²,

McGee³

et al. 1987

Journal of Endocrinology

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“…In a seminal vesicle fibromuscular fraction from adult guinea-pigs, castration had no effect on DNA or collagen content. Exogenous dihydrotestosterone given to guinea-pigs increased fibromuscular collagen levels to supranormal values (Mariotti & Mawhinney, 1982); oestradiol benzoate increased fibromuscular DNA and collagen to supranormal levels (Mariotti & Mawhinney, 1981. Most studies do not distinguish between fibrous tissue (mainly in the interacinar tissue and lamina propria) and muscular tissue (in the muscular stroma of the hamster) and so it is difficult to draw direct comparisons between studies and across species.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies do not distinguish between fibrous tissue (mainly in the interacinar tissue and lamina propria) and muscular tissue (in the muscular stroma of the hamster) and so it is difficult to draw direct comparisons between studies and across species. However, it appears that, in some rodents at least, stromal collagen content is controlled by both androgens and oestrogens and stromal cell numbers are controlled by oestrogens (Mariotti & Mawhinney, 1981.…”

Section: Discussionmentioning

confidence: 99%

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Architecture of the dorsal and ventral lobes of the prostate of the Syrian hamster, Mesocricetus auratus, after regrowth from short day-induced regression

Buzzell

1989

Reproduction

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The structures of the dorsal and ventral lobes of the prostate of 4 groups of Syrian hamsters were studied by a stereological approach. Groups studied were young hamsters (kept in long-day photoperiods for 4 weeks) in their first breeding season, older hamsters (kept in short-day photoperiods for 24 weeks so that their prostates had regressed and regrown) in their second breeding season, and two groups of older hamsters in an extended first breeding season (either because they were kept in long days for 24 weeks or because they were pinealectomized before being put into short-day photoperiods for 24 weeks). There were very few differences between groups, but generally dorsal prostates of older hamsters in their second breeding season closely resembled those of young hamsters in their first breeding season. More differences were noted between either of these two groups and older hamsters in an extended first breeding season than between these two groups. The differences noted generally involved increases in the amount of smooth muscle in the walls of secretory tubules in the dorsal prostates of hamsters in an extended first breeding season. This may be associated with the fact that these glands had not regressed and regrown. Ventral prostates were very similar in all 4 groups, which may reflect the fact that they normally regress very little in short days.

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“…Both in vitro and in vivo studies suggest that these receptors are involved in the regulation of the motility of the male genital tract (15). Seminal vesicles have a fibromuscular stroma that appears to be a target for androgens and estrogens (16). To test the hypothesis that these glands are also a target tissue for neurohypophysial hormones, we studied the binding of labeled OT and arginine vasopressin (AVP) and the response of adenylate cyclase to these hormones in membranes prepared from porcine seminal vesicles.…”

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confidence: 99%

Identification and characterization of a vasopressin isoreceptor in porcine seminal vesicles.

Maggi¹,

Kassis²,

Malozowski³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Neurohypophysial hormones stimulate the motility of tunica albuginea, epididymis, and vas deferens acting through oxytocin (OT) and V1 vasopressin receptors. To test the hypothesis that these hormones are involved also in the regulation of seminal vesicle physiology, we studied binding of properties are distinct from the previously described V1 and V2 vasopressin receptors and indicate the presence of a new class of AVP receptors. Although this vasopressin isoreceptor shares some pharmacological characteristics with the V1 (pressor) isoreceptor, it has low affinity for the V1 antagonist d(CH2)5-Tyr(Me)AVP and is linked to the adenylate cyclase system. The extremely high density of AVP receptors in porcine seminal vesicles (2 pmol per mg of protein) is comparable to the density of V2 vasopressin receptors in porcine renal medulla, suggesting a physiological role for vasopressin in the seminal vesicle.Vasopressin receptors have been identified and characterized in several mammalian tissues: kidney (1), liver (2), vascular smooth muscle cells (3), brain (4), pituitary gland (5), and the interstitial cells of the rat testis (6). Mitchell et al. (7) postulated the presence of two vasopressin isoreceptors, distinguished on the basis of functional criteria. The V1 subtype of receptors regulates cellular calcium metabolism, whereas the V2 subtype is functionally coupled to adenylate cyclase (1). The recent development of potent and selective ligands for these two isoreceptors allowed the identification of V1 isoreceptors in blood vessels, liver, brain, and interstitial cells of the testis (2-4, 6), whereas the V2 isoreceptor has been localized in the renal medulla (1).Oxytocin (OT) receptors were described in the uterus (8), mammary gland (9), oviduct (10), and hippocampus (11). These receptors, in mammals, are not functionally coupled to adenylate cyclase (12); it has been suggested that the occupancy of OT binding sites is associated with an increase of intracellular calcium, apparently via specific inhibition of Ca2+,Mg2+-ATPase (13).We have recently identified and characterized OT and V1 vasopressin receptors in the tunica albuginea of the testis, epididymis, and vas deferens ofthe prepubertal pig (14). Both in vitro and in vivo studies suggest that these receptors are involved in the regulation of the motility of the male genital tract (15). Seminal vesicles have a fibromuscular stroma that appears to be a target for androgens and estrogens (16). To test the hypothesis that these glands are also a target tissue for neurohypophysial hormones, we studied the binding of labeled OT and arginine vasopressin (AVP) and the response of adenylate cyclase to these hormones in membranes prepared from porcine seminal vesicles. MATERIALS AND METHODS[3H]AVP Ci/mmol; 1 Ci = 37 GBq) and [3H]OT 8824The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Hormonal control of accessory sex organ fibromuscular stroma

Cited by 20 publications

References 15 publications

Effects of ageing and hormonal manipulations on the level of oestrogen receptors in the guinea-pig prostate

Effects of ageing and hormonal manipulations on the level of oestrogen receptors in the guinea-pig prostate

Architecture of the dorsal and ventral lobes of the prostate of the Syrian hamster, Mesocricetus auratus, after regrowth from short day-induced regression

Identification and characterization of a vasopressin isoreceptor in porcine seminal vesicles.

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