Hormonal induction of ovulation in pikeperch (Sander lucioperca L.) using human chorionic gonadotropin (hCG) and mammalian GnRH analogue

Abstract

The aim of the present study was to study spawning stimulation in artificial reproduction of females pikeperch (Sander lucioperca L.) using ''Chorulon'' containing the human chorionic gonadotropin (hCG) and compare with ''Supergestran'' containing a mammalian GnRH ([D-Ala 6 ]GnRHProNhet) analogue. The females were divided into eleven experimental groups and injected with hCG at 250, 500, 750, and 1 000 IU kg -1 body weight (BW) and mGnRHa at 1, 2.5, 5, 10, 25, and 50 lg kg -1 BW. In all treatments, a single intramuscular injection of hormone was performed. Control group was injected with 0.9 % NaCl, 0.9 cm 3 kg -1 BW. The average percentages of ovulating females were 88.5 ± 12.3 and 80.8 ± 10.9 % in hCG-and mGnRHa-treated groups, respectively. The average diameter of eggs was 0.95 ± 0.06 and 0.98 ± 0.06 mm in hCG-and mGnRHatreated groups, respectively. Neither ovulation rate nor diameter of egg was statistically differed among hormonally treated groups. Statistical difference was observed only in hatching rate, where the average were 73.6 ± 14.4 and 50.6 ± 17.7 % in hCG and mGnRHa-treated groups, respectively. Among hormonally treated groups, the best results were observed in groups treated with hCG at 500 and 750 IU kg -1 and in groups treated with mGnRHa at 25 lg kg -1 . No ovulation was observed in the control group. This study indicated successful ovulation in pikeperch using a single intramuscular injection of hCG or mGnRHa analogue.

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“…Hormonal stimulation is applied differently in two groups of cyprinid fish (Podhorec and Kouril, 2009). In fish with one-off spawning, carp pituitary homogenate (CPH) is usually used in in-season reproduction, as well as preparations containing GnRH analogue with dopamine inhibitor (DA) or combinations of CPH with hCG (Kucharczyk et al, 2005;Brzuska, 2005Brzuska, , 2006Kristan et al, 2013;Nowosad et al, 2014b). In batch-spawning fish, like in percids (such as perch Perca fluviatilis L.), there can be other agents used successfully except for the preparations mentioned above, such as hCG, FSH+LH, or analogues of GnRH (Kucharczyk et al, 1998;Targońska and Kucharczyk, 2011;Targońska et al, 2012a).…”

The substrate selection and spawning behaviour of pikeperch Sander lucioperca L. broodstock under pond conditions

“…Longer preparation could have a reflection in fertilization rate as the spawning substrates are suspected to stimulate the papilla of the male during nest cleaning.Although hormone stimulation of pikeperch broodstock is often recommended in aquaculture to synchronize spawning(Kristan, Alavi, Stejskal, & Policar, 2013;Samarin, Blecha, Bytyutskyy, & Policar, 2015;Zarski et al, 2015) in this study, spawning was not hormone-T A B L E 2 Water temperature dynamic during 14 days of the experiment. This finding did not affect other parameters of pikeperch reproduction; however, it may be interesting from an ecological point of view.…”

Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media

“…Sperm was collected from 12 mature pikeperch (body mass 871.58 ± 31.00 g (mean ± standard deviation), total length 466.9 ± 41.0 mm) 48 h after spermiation induction using a single intramuscular injection of hCG at 500 IU/kg (Křišťan et al, 2013). Spermatozoa concentration was assessed by light microscopy, using a Burker haemocytometer (Marienfield, Konigshofen, Germany), following the method of Alavi et al (2009a).…”