Hormonal influences of detoxication in the rat ovary on enzymes in comparison with the liver

Characterization of the UDP-glucuronosyltransferase isoenzyme expressed in rat ovary and its regulation by gonadotropins

“…Microsomes from the liver of PMSG-hyperstimulated rats were also analysed by immunoblotting. No differences compared with liver microsomes from untreated mature female rats were seen, in agreement with activity measurements [11] (Figure 1, bottom). Immunochemical analysis of microsomes prepared from porcine ovarian follicles of different sizes revealed the presence of only phenol UGT in this organ.…”

“…PMSG treatment was as follows : immature (26-day-old) rats were treated with 10 IU of PMSG to synchronize the preovulatory and luteal phases. Mature (250 g) rats were hyperstimulated with 150 IU of PMSG [11]. Alternatively, the hormone treatment was as follows : immature rats were injected subcutaneously with β-oestradiol (10 µg\rat) or progesterone (1 mg\rat) dissolved in sesame oil, once daily from 26 to 28 days of age between 08 : 00 and 10 : 00 h. Another group received a single injection of hCG (10 IU\rat) dissolved in 0n9 % NaCl at 15 : 00 h on the 28th day of postnatal life.…”

“…UGT was measured radiometrically in the microsomal fraction by the methods of Hjelle et al and Otani et al [18,19] as modified by Becedas and Bengtsson Ahlberg [11] : 0n5-1n0 mg of protein, a final substrate concentration of 500 µM 1-["%C]naphthol (0n04 µCi), and 8 mM CHAPS in 100 mM Tris\HCl, pH 7n4, were used. The reaction was started by the addition of 3 mM UDP-glucuronic acid and terminated with 0n13 M trichloroacetic acid and 0n2 M glycine, pH 2n7.…”

“…pregnant mare's serum gonadotropin (PMSG, exhibiting equal LH and FSH activities) [11]. In the present study, we investigated whether this regulation of UGT activity reflects changes in the amount of protein and\or mRNA.…”

Induction of NAD(P)H quinone oxidoreductase and glutathione S-transferase activities in livers of female August-Copenhagen Irish rats treated chronically with estradiol: comparison with the Sprague–Dawley rat

“…Since changes in SULT1A1 activity have been observed in uterus but not in liver of rats throughout the menstrual cycle [39], this enzyme may be regulated by estrogen in a tissue-specific manner. Moreover, changes in expression of other SULTs, for example SULT1E and SULT2A isoforms, responsible for sulfonation of estrogens and hydroxysteroids, respectively, cannot be ruled out.…”