Hormonal regulation of pregnancy  and related behavioral changes in rabbits

Chmurska-Gasowskia, M.; Sowińska, Natalia

doi:10.21521/mw.6332

Cited by 1 publication

(2 citation statements)

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“…The use of pseudopregnant local rabbits as ovarian transplant recipients in this study was appropriate, as Sumarmin [ 11 ] reported previously that pseudopregnant rabbits can be used as a source of oocytes for ovarian transplantation. Despite the use of rabbits in various studies, such as those of reproductive endocrinology [ 9 ] and hormone receptors [ 10 ], in the present study, we focused on using rabbits for interspecies ovarian transplantation. The results of the ovarian transplantation performed here revealed the presence of several follicles that contained intact oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian transplantation can be achieved using experimental animals, such as rabbits. Rabbits are an animal model that is commonly used in studies of reproductive endocrinology [ 9 ], detection of hormone receptors [ 10 ], and ovarian transplantation, as a source of oocytes [ 11 ]. Ovarian transplantation can be performed on pseudopregnant rabbits because, at the beginning of pregnancy, the glands found in the uterine endometrium of rabbits actively produce and stimulate secretions into the uterine lumen, as a nutritional reserve for the embryo before implantation.…”

Section: Introductionmentioning

confidence: 99%

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Transplantation of Aceh cattle ovary into the uterus of pseudopregnant local rabbits: Effect of post-transplant stress on uterine histopathology and ovarian follicle dynamics

et al. 2023

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Background and Aim: The increase in the levels of the cortisol hormone caused by the stress conditions generated by an ovary transplantation procedure can damage the uterus of the transplant recipient as well as the transplanted ovaries. This study aimed to analyze the histopathological changes that occur in the uterine horn of pseudopregnant local rabbits (recipients), as well as the ovarian follicular integrity of the donor Aceh cattle after transplantation. Materials and Methods: After 30 days of adaptation, all rabbits were divided into three treatment groups: R1 (the group of rabbits that underwent ovarian transplantation for 3 days, n = 5), R2 (the group of rabbits that underwent ovarian transplantation for 5 days, n = 5), and R3 (the group of rabbits that underwent ovarian transplantation for 7 days, n = 5). Pseudopregnancy induction was performed using the pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) methods. The rabbits were injected with 100 IU of PMSG intramuscularly, followed by an injection of 75 IU of hCG intravenously 3 days later. Ovarian transplantation was performed on day 8 (day 0 was the day of hCG injection). The concentration of cortisol hormone metabolites was measured from fecal samples using an enzyme-linked immunosorbent assay technique. The uterus and ovaries were collected for histopathological and follicular dynamics examination after the transplantation process was completed. Results: The mean cortisol levels (ng/g) recorded before versus after the transplant in the R1, R2, and R3 groups were 146.23 ± 17.60 versus 338.84 ± 302.79, 128.97 ± 81.56 versus 174.79 ± 101.70, and 124.88 ± 43.61 versus 321.91 ± 221.63 (p < 0.05), respectively. The examination of the histopathological appearance of the uterus revealed edema in the uterine lumen, hyperemia and hemorrhage in the endometrium, necrosis of the epithelium, and infiltration of inflammatory cells. Hemorrhage and hyperemia were severe and filled the endometrium in the R1 compared with the R2 and R3 animals. Ovarian follicle development occurred in all treatment groups, although some histopathological features were observed. The number of tertiary follicles in R1, R2, and R3 animals was 24.67 ± 7.37, 20.67 ± 7.57, and 9.67 ± 3.79 (p < 0.05), respectively. Conclusion: Based on the results of this study, it can be concluded that the transplantation of ovaries from Aceh cattle into pseudopregnant local rabbits triggered an increase in the levels of the cortisol hormone and uterine histological changes; however, follicles were still detected at various stages of development in the transplanted Aceh cattle ovaries. The results of this study are valuable for clinicians and researchers because they provide information regarding an alternative in vivo ovarian preservation technique using pseudopregnant rabbits. Keywords: cortisol, ovarian, transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%