Hormonal regulation of vascular endothelial growth factor A (VEGFA) gene expression in granulosa and theca cells of cattle1

Nichols, Jacqueline; Perego, Maria Chiara; Schütz, Luís F.; Hemple, Amber M; Spicer, L. J.

doi:10.1093/jas/skz164

Cited by 20 publications

(12 citation statements)

References 83 publications

(136 reference statements)

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“…The VEGFA gene, which encodes a vascular endothelial growth factor is upregulated in cancer tumors and its expression is correlated with tumor stage and progression [ 35 ]. In bovine, the VEGFA gene has also been associated with angiogenesis and increased vascularity in ovarian follicles [ 36 , 37 ], being considered an important regulator of placental development and function [ 38 ]. This gene plays an important function in the better supply of oxygen and nutrients to tissues, as well as in the tissue repair process after damage [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…bovine, the VEGFA gene has also been associated with angiogenesis and increased vascularity in ovarian follicles [36,37], being considered an important regulator of placental development and function [38]. This gene plays an important function in the better supply of oxygen and nutrients to tissues, as well as in the tissue repair process after damage [39].…”

Section: Plos Onementioning

confidence: 99%

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Genome-wide association study for mammary structure in Canadian Angus cows

et al. 2020

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Functional and enduring mammary structure is pivotal for producer profitability, and animal health and welfare in beef production. Genetic evaluations for teat and udder score in Canadian Angus cattle have previously been developed. The aim of this study was to identify genomic regions associated with teat and udder structure in Canadian Angus cows thereby enhancing knowledge of the biological architecture of these traits. Thus, we performed a weighted single-step genome wide association study (WssGWAS) to identify candidate genes for teat and udder score in 1,582 Canadian Angus cows typed with the GeneSeek ® Genomic Profiler Bovine 130K SNP array. Genomically enhanced estimated breeding values (GEBVs) were converted to SNP marker effects using unequal variances for markers to calculate weights for each SNP over three iterations. At the genome wide level, we detected windows of 20 consecutive SNPs that explained more than 0.5% of the variance observed in these traits. A total of 35 and 28 windows were identified for teat and udder score, respectively, with two SNP windows in common for both traits. Using Ensembl, the SNP windows were used to search for candidate genes and quantitative trait loci (QTL). A total of 94 and 71 characterized genes were identified in the regions for teat and udder score, respectively. Of these, 7 genes were common for both traits. Gene network and enrichment analysis, using Ingenuity Pathway Analysis (IPA), signified key pathways unique to each trait. Genes of interest were associated with immune response and wound healing, adipose tissue development and morphology, and epithelial and vascular development and morphology. Genetic architecture from this GWAS confirms that teat and udder score are distinct, polygenic traits involving varying and complex biological pathways, and that genetic selection for improved teat and udder score is possible.

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Section: Resultsmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Genome-wide association study for mammary structure in Canadian Angus cows

et al. 2020

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“…The results of the KEGG analysis indicated that TGFβ1I1 is involved in the regulation of the VEGF signaling pathway. VEGFA, a gene encoding a heparin-binding protein, plays an important role in angiogenesis by inducing the proliferation and migration of vascular endothelial cells [ 52 ]. VEGF, as an angiogenic factor, binds to corresponding receptors on the follicular granulosa cell membrane to trigger downstream signaling cascades and promote follicle development [ 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miR-33 Exacerbates Heat-Stress-Induced Apoptosis in Granulosa Cell and Follicular Atresia of Nile Tilapia (Oreochromis niloticus) by Targeting TGFβ1I1

Qiang

Tao

et al. 2022

Genes

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High temperature affects egg quality and increases follicular atresia in teleosts. The present study aimed to explore the regulated mechanism of ovary syndrome of Nile tilapia (Oreochromis niloticus) exposed to heat stress. To this end, we conducted histological and biochemical analyses and integrated miRNA-target gene analyses. The histochemical analyses confirmed that heat stress promoted the apoptosis of granulosa cell and therefore resulted in increased follicular atresia in the ovary. Heat stress led to the differential expression of multiple miRNAs (miR-27e, -27b-3p, -33, -34a -133a-5p, and -301b-5p). In a luciferase activity assay, miR-33 bound to the 3′-untranslated region (UTR) of the TGFβ1I1 (transforming growth factor-β1-induced transcript 1) gene and inhibited its expression. A TGFβ1I1 gene signal was detected in the granulosa cells of Nile tilapia by immunohistochemical analysis. Up-regulation of the miR-33 of tilapia at 6 d and 12 d exposed to heat (34.5 °C ± 0.5 °C) had significant down-regulation of the TGFβ1I1 expression of the gene and protein in tilapia ovaries. An miRNA-target gene integrated analysis revealed that miR-33 and TGFβ1I1 function in an apoptosis-related signal pathway. The signal transduction of the vascular endothelial growth factor (VEGF) family members VEGFA and its receptor (KDR) in the heat-stressed group decreased significantly compared with the control group. Transcript-levels of the Bax and Caspase-3 as apoptotic promotors were activated and Bcl-2 and Caspase-8 as apoptotic inhibitors were suppressed in the heat-stressed tilapia. These results suggest that heat stress increases the expression of miR-33, which targets TGFβ1I1 and inhibits its expression, resulting in decreased levels of follicle-stimulating hormone and 17β-estradiol and increased apoptosis by suppressing VEGF signaling, eventually inducing follicular atresia. In conclusion, our results show that the miR-33/TGFβ1I1 axis of Nile tilapia is involved in the follicular development of broodstock, and can suppress VEGF signaling to accelerate follicular atresia. Our findings demonstrate the suppressive role of miR-33 during oocyte development in Nile tilapia.

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“…The thecal vasculature is well developed in healthy follicles and breaks down in atretic follicles. The major proangiogenic factors, FGF2 and vascular endothelial growth factor (VEGF), are expressed in follicles and are under endocrine or paracrine control, as demonstrated by the ability of BMP4 to stimulate VEGFA mRNA levels in vitro in the bovine theca (Nichols et al 2019). In a bovine theca-endothelial cell coculture system, endothelial tubes form during culture, and angiogenesis is inhibited by TGFB1 or BMP6 and stimulated by a combination of VEGF and FGF2 (Mattar et al 2020).…”

Section: Resurrection Of the Thecamentioning

confidence: 99%

The ovarian follicle of ruminants: the path from conceptus to adult

Juengel

Cushman²,

Dupont

et al. 2021

Reprod. Fertil. Dev.

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This review resulted from an international workshop and presents a consensus view of critical advances over the past decade in our understanding of follicle function in ruminants. The major concepts covered include: (1) the value of major genes; (2) the dynamics of fetal ovarian development and its sensitivity to nutritional and environmental influences;(3) the concept of an ovarian follicle reserve, aligned with the rise of anti-Müllerian hormone as a controller of ovarian processes; (4) renewed recognition of the diverse and important roles of theca cells; (5) the importance of follicular fluid as a microenvironment that determines oocyte quality; (6) the 'adipokinome' as a key concept linking metabolic inputs with follicle development; and (7) the contribution of follicle development to the success of conception. These concepts are important because, in sheep and cattle, ovulation rate is tightly regulated and, as the primary determinant of litter size, it is a major component of reproductive efficiency and therefore productivity. Nowadays, reproductive efficiency is also a target for improving the 'methane efficiency' of livestock enterprises, increasing the need to understand the processes of ovarian development and folliculogenesis, while avoiding detrimental trade-offs as greater performance is sought.

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Hormonal regulation of vascular endothelial growth factor A (VEGFA) gene expression in granulosa and theca cells of cattle1

Cited by 20 publications

References 83 publications

Genome-wide association study for mammary structure in Canadian Angus cows

Genome-wide association study for mammary structure in Canadian Angus cows

Upregulation of miR-33 Exacerbates Heat-Stress-Induced Apoptosis in Granulosa Cell and Follicular Atresia of Nile Tilapia (Oreochromis niloticus) by Targeting TGFβ1I1

The ovarian follicle of ruminants: the path from conceptus to adult

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