Hormonal treatment affects sperm motility in the spined loach (Cobitis taenia, Pisces, Cobitidae)

Sperm motility analysis of Cobitis hankugensis, Iksookimia longicorpa (Teleostei, Cypriniformes, Cobitidae) and their unisexual natural hybrids

“…The velocity parameters of C. hankugensis and I. longicorpa were relatively lower (for VAP, 70.0 and 64.9 μm/s, respectively) than that of the other Cyprinid fishes, A. alburnus (72-117 μm/s; Lahnsteiner et al, 1996), C. carpio (73-105 μm/s;Perchec-Poupard et al, 1997), and T. tinca (120-140 μm/s;Linhart et al, 2003). Most of all, although performed with well-evaluated activation solutions (Kucharczyk et al, 2008;Lahnsteiner et al, 1996;Perchec-Poupard et al, 1996;Woynarovich & Woynarovich, 1980), C. taenia, which belongs to the same Cobitid, exhibited a significantly high-velocity value about twice as much as our study (Cejko et al, 2017). Whereas, we used an activation solution that was adjusted to equal the salinity and osmotic pressure of the environmental water in the fish habitat to minimize artificial interference.…”

“…In addition, these results were not different for other species including M. anguillicaudatus (88%–95%; Zhao et al, 2012), (93%–100%; Linhart et al, 2005), (80.71%; Tian et al, 2015), and Xiphophorus helleri (90%–95%; Yang et al, 2007). Interestingly, hormone‐stimulated C. taenia showed 98.8 ± 0.8% and 88.2 ± 11.5% motility (for hCG and Ovopel, respectively; Cejko et al, 2017), whereas semen was not collected from the control group. However, spermatozoa with high motility were seen in C. hankugensis and I. longicorpa without any hormone treatment at least during the spawning season.…”

Effects of hCG and salmon gonadoliberine analogue on spermiation in the Eurasian perch ( Perca fluviatilis )

“…From several motility parameters measured using the CASA system (Sperm Class Analyzer v. 4.0.0. by Microptic S.L., Barcelona, Spain), spermatozoa motility (MOT, %), beat cross frequency (BCF, Hz), curvilinear velocity (VCL, mm s-1), amplitude of lateral head displacement (ALH, mm), straight linear velocity (VSL, mm s-1) and linearity of movement (LIN, %) were chosen as the most widely-used quality indicators of sperm quality [8,14]. Sperm was activated on the Makler chamber (Sefi-Medical Instruments ltd., Israel) using a solution composed of 75 mM NaCl, 2 mM KCl, 1 mM MgSO4$7H2O, 1 mM CaCl2$2H2O, 20 mM Tris, pH 8 (modified Lahnsteiner's activating solution, as described by Bernath et al [25]).…”

“…However, without stimulation, low quantity and/or quality of the sperm is very often observed [5,6]. It has been reported that the type of spawning agent [14,15] as well as the latency time (the time period between hormonal injection and sperm collection) [16,17] are the two major factors directly effecting the quantity and quality of the sperm in freshwater teleosts. This was especially important for RASreared fish like the common barbel, Barbus barbus L., where hormonal therapy obtained two-fold more sperm of very high quality compared to the non-stimulated fish [18].…”