Hormone receptor status and HER2/neu overexpression determined by automated immunostainer on routinely fixed cytologic specimens from breast carcinoma: Correlation with histologic sections determinations and diagnostic pitfalls

Moriki, Toshiaki; Takahashi, Tamotsu; Ueta, Shousuke; Mitani, Miko; Ichien, Miho

doi:10.1002/dc.20007

Cited by 37 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In regard to the overall HR status, one case showed a discordant negative result, in which a few positive cells were found in the histological section. Previous studies have pointed out the intratumoral heterogeneity of positive cells as the main reason for discordant results between cytologic and histologic specimens [7,9,12,14,15] similar to the case in our results. One study suggested that the mucin over the mucinous carcinoma cells may have prevented proper immunostaining [15].…”

Section: Discussionsupporting

confidence: 91%

“…Several studies have reported on assessment of the HR status using cytological specimens [6][7][8][9][10][11][12][13][14][15][16][17][18]. From the mid 1980s to the late 1990s, the HR status in cytological specimens was reported to show a good correlation with that of biochemical assays [9,11,12,19].…”

Section: Discussionmentioning

confidence: 99%

“…From the mid 1980s to the late 1990s, the HR status in cytological specimens was reported to show a good correlation with that of biochemical assays [9,11,12,19]. More recently, several studies have reported comparison of the HR status by IHC in cytological specimens and frozen [8,10] or paraffinembedded [7,11,13,15,17,18,20] histological sections. All of the literature published until now indicates the existence of a good correlation between the HR status in cytological specimens and that in the corresponding surgical specimens.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

et al. 2010

View full text Add to dashboard Cite

Immunohistochemical (IHC) analysis of the hormone receptor (HR) in breast cancer cytology is an important issue nowadays. Several studies have shown discrepancy in the HR status between the primary tumor and metastases. Cytology can be used for patients with metastatic disease. Although cytological assessment of HR is an excellent method, it has not been routinely used because of the difficulty in consistently preparing multiple good quality slides. Liquid-based cytology (LBC) preparation is considered as the key to resolving the aforementioned problem; however, few studies have reported the HR assessment in breast cancer using LBC. Therefore, the HR status of LBC slides from 82 breast cancers was compared with that of the corresponding surgical specimens. The HR assay in both the LBC slides and surgical specimens was conducted by IHC using an autostainer. For the IHC staining, the protocol recommended by the manufacturer for paraffin-embedded sections was used for both the cytology and histology specimens. The HR results of the cytology agreed with those of the histology in 80 of the 82 cases (accuracy rate, 98%) for estrogen receptor, and in 78 of the 82 cases (accuracy rate, 95%) for progesterone receptor. The overall accuracy of the HR status on the cytology and the histology was 99% in 81 of the 82 cases. In conclusion, in HR analysis of breast cancers, LBC followed by IHC using an autostainer was useful for the standard processing of cytological specimens and showed a good correlation with the results of analysis on the histology specimens.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Furthermore, the use of cell blocks has provided material for ancillary studies including the analysis of hormone receptors (ER, PR) and HER2 status, and for immunostains (i.e. cytokeratin) that can help support the presence of metastatic carcinoma in difficult cases [5,6,7,8,9]. In addition, the effectiveness of FNA has been optimized with the use of real-time ultrasound guidance that has helped to target specific areas of concern, to confirm the needle location in the LN, and to facilitate interactions between radiologists and pathologists to improve the quality of the FNA sample [10,11].…”

Section: Introductionmentioning

confidence: 99%

The Role of Axillary Lymph Node Fine Needle Aspiration in Breast Cancer Staging prior to Chemotherapy with Histological Correlation

Colaizzi¹,

Kanbour²,

Ibrahim³

et al. 2012

Acta Cytologica

View full text Add to dashboard Cite

Objective: Axillary lymph node (LN) sampling is important for breast cancer staging and can be performed using fine needle aspiration (FNA). The aim of this study is to review the axillary LN FNAs performed at our institution prior to treatment, and to correlate their results with the available LN histology in order to evaluate the diagnostic utility of FNA. Study Design: A total of 130 cases were retrospectively identified over a 1-year period and reviewed. The FNA findings were correlated with the histological findings in 65 (50%) LN core biopsies, 43 (33%) sentinel LN biopsies, and 22 (17%) axillary dissections. Results: One hundred and thirteen FNA cases (89%) correlated with the histology, while 14 cases (11%) did not correlate, including 1 false positive and 13 false negatives. Of the false-negative cases, metastases in the biopsy ranged from isolated tumor cells to 5 mm in the greatest dimension. The overall sensitivity was 85%, specificity 98%, positive predictive value 99%, and negative predictive value 75%. Conclusion: Pretherapy staging of breast cancer by FNA is a useful diagnostic modality with a high specificity and positive predictive value. Due to false-negative diagnoses, a negative FNA should be followed up with further LN sampling.

show abstract

“…The polysomy 17 and HER2 heterogeneity may contribute to a better understanding of HER2-positive tumours and its variation in therapeutic responses. Furthermore, this could also explain the confl icting data in studies about the prognostic and predictive role of HER2 status in breast cancer patients [23].…”

Section: Discussionmentioning

confidence: 99%

HER2 status in breast cancer: experience of a Spanish National Reference Centre

et al. 2011

View full text Add to dashboard Cite

show abstract

Hormone receptor status and HER2/neu overexpression determined by automated immunostainer on routinely fixed cytologic specimens from breast carcinoma: Correlation with histologic sections determinations and diagnostic pitfalls

Cited by 37 publications

References 34 publications

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

The Role of Axillary Lymph Node Fine Needle Aspiration in Breast Cancer Staging prior to Chemotherapy with Histological Correlation

HER2 status in breast cancer: experience of a Spanish National Reference Centre

Contact Info

Product

Resources

About