Hormone receptors in gastric cancer

Tokunaga, Akira; Kojima, Naoto; Andoh, Toshiaki; Matsukura, Norio; Yoshiyasu, Masayuki; Tanaka, Noritake; Ohkawa, K; Shirota, Akiro; Asano, Gorō; Hayashi, Kazuo

doi:10.1016/0277-5379(83)90186-4

Cited by 48 publications

(27 citation statements)

References 5 publications

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“…Our results agree with those of Kuramoto et al (27) who failed to detect any ERc~ in confluent Capan-1 ceils or in the heterotransplants in female nude mice, whereas cell lines known to be poorly to moderately differentiated such as Capan-2, BxPc-3, and Mia-PaCa-2, expressed ERtx even at confluence. Our observations are consistent with those in nontarget tissues such as gastric cancers where ERa and PR have been found to be expressed in undifferentiated tumors (46), faintly expressed in poorly differentiated tumors (25), and undetectable in differentiated tumors (31,46). Furthermore, Utsumi et al (47) have reported a low level of ERa expression in esophageal tumor ceils of the Es-25C line, which are regarded as moderately differentiated.…”

Section: Discussionsupporting

confidence: 94%

“…These differences may stem from differences in procedures of assay or characterization, or to the degree of differentiation of the tumors or ceil line studied. Recently for the tumors of the digestive tract, some authors have described a relationship between the expression of steroid hormone receptors and the stage of differentiation (25,46). A link between the expression of glucocorticoid receptors and state of differentiation has also been suggested (39).…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, expression of ERR has been found in tumors derived from nonestrogen target tissues, including glioblastomas (48), osteoblastlike osteosarcoma (34), tumors of the thyroid (23,35), kidney (11), ~To whom correspondence should be addressed. and digestive tract from esophagus (47), liver (38), and stomach (25,31,46) to colon (33,42). For cancers of the pancreas, the reports are conflicting.…”

Section: Introductionmentioning

confidence: 99%

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Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation

Hollande

Fanjul

Houti

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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In steroid target tissues, the presence of the corresponding hormone receptors is indicative of hormone dependence. In an attempt to assess the possible role of steroid hormones in the mechanism of growth and/or differentiation of cancerous pancreatic duct cells, the expression of estrogen receptor (ERalpha) was evaluated in human cancerous pancreatic duct cells (Capan-1) maintained in culture. These cells were selected as they acquire progressively a high degree of differentiation during growth in culture. In the present study, we showed that Capan-1 cells during growth in steroid-free medium associate spontaneously, become polarized, and form duct-like structures, features that are indicative of a high degree of differentiation. Capan-1 cells were also found to express ERalpha and progesterone receptor (PR). Immunoenzymatic assay showed maximal expression of ERalpha (236 +/ 55 fmol/mg protein) on the first day of the exponential growth phase, followed by a marked fall in expression (76.3%). At the onset of the stationary phase (Day 5), ERalpha levels were below 10 fmol/mg protein, becoming undetectable by Day 7. A similar time course was observed for PR: 18 +/- 0.9 fmol/mg protein at the onset of the exponential growth phase and no expression during the stationary phase. Addition of estradiol to 1-d-old cultures resulted in a twofold increase in PR expression, suggesting an induction of PR expression by estrogen. Immunocytochemical analysis with anti-ERalpha-1D5 antibodies showed nuclear and cytoplasmic localization of ERalpha in Capan-1 cells in the first 24 h of culture followed by a progressive disappearance thereafter. We also showed that cellular multiplication was increased by estradiol and progesterone during the exponential growth phase, pointing to the involvement of steroid hormones in the proliferation of nonpolarized Capan-1 cells. These results indicate that the expression of ERalpha is linked to the state of differentiation of the cells and make Capan-1 cells a model of choice to study ER regulation in nontarget tissues.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation

Hollande

Fanjul

Houti

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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“…The relationship between hormone receptors and GC was first reported by Tokunaga et al [5]. Since then, mounting studies have investigated the expression of sex hormone receptors including estrogen receptor alpha (ERα), estrogen receptor beta (ERβ) and androgen receptor (AR), as well as their prognostic implications in GC [6–20].…”

Section: Introductionmentioning

confidence: 99%

Expression of estrogen receptors and androgen receptor and their clinical significance in gastric cancer

et al. 2017

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Despite the mounting studies exploring the role of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ) and androgen receptor (AR) in gastric cancer (GC), there remain controversies in those findings. The present study investigated the expression of ERα, ERβ and AR in Chinese gastric cancer by immunohistochemistry, analyzed their clinical relevance in gastric cancer, and examined the potential mechanisms by which ERα and AR modulated GC progression. The positive rate of ERα, ERβ and AR in GC tissues was 6% (9/150), 93.5% (143/153), and 42.4% (59/139), respectively. The expression of ERα was an independent unfavorable risk factor for overall survival (OS) (hazard ratio [HR] = 3.639, 95% confidence interval [CI] = 1.432-9.246, p = 0.007) for GC patients. Moreover, AR was borderline significantly associated with poor progress free survival (PFS) after adjustment with other variables (HR = 1.573, 95% CI = 0.955-2.592, p = 0.075). Knockdown of ERα inhibited the proliferation, migration and invasion of GC cells possibly via modulating the expression of p53, p21, p27, cyclin D1 and E-cadherin. Downregulation of AR suppressed the migration and invasion of GC cells and inhibited the epithelial-mesenchymal transition (EMT) associated pathways.ConclusionThe present study showed that positive ERα was associated with poor prognosis of Chinese GC patients. ERα might modulate the proliferation, migration and invasion via regulating the expression of p53, p21, p27, cyclin D1 and E-cadherin. ERα could be a valuable prognostic biomarker and promising therapeutic target for Chinese GC patients.

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“…Recently, hormone receptors have been found in various benign and malignant tissues including gastric tumors [1], lung tumors [2,3] and other tumors [4] which have been thought to be nontarget organs for hormones. Thus, analyses of hormone receptors in these types of tumors are important because such investigations may extend the applicability of endo crine therapies.…”

Section: Introductionmentioning

confidence: 99%

Influence of Hormone Therapy on Human Lung Tumors Transplanted into Nude Mice

et al. 1985

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Specific estrogen, progesterone, androgen and glucocorticoid receptors were determined in 8 lung tumor lines transplanted into athymic nude mice. It was found that the receptor content for estrogen, progesterone or androgen was very low for all tumor lines tested, whereas the glucocorticoid receptor content was high in all cases except 1. The growth inhibition of lung carcinomas on nude mice by glucocorticoid or antiglucocorticoid treatment suggests that lung cancer may also be influenced by hormonal therapy.

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Hormone receptors in gastric cancer

Cited by 48 publications

References 5 publications

Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation

Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation

Expression of estrogen receptors and androgen receptor and their clinical significance in gastric cancer

Influence of Hormone Therapy on Human Lung Tumors Transplanted into Nude Mice

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