Hormone Receptors: Studies on the Interaction of Estrogen with the Uterus

Gorski, Jack; Toft, David O.; Shyamala, G.; Smith, Donald E.; Notides, Angelο C.

doi:10.1016/b978-1-4831-9827-9.50008-3

Cited by 250 publications

(164 citation statements)

References 10 publications

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“…The crossreactivity of antibodies to the purified estrophilin from calf nuclei with extranuclear as well as nuclear estradiol-receptor complexes from calf and rat uterus indicates an immunochemical similarity between the two forms of estrophilin and provides supporting evidence for the concept of a two-step interaction mechanism in which the nuclear receptor is derived from the hormone-induced translocation of the extranuclear receptor (13,14). The apparent formation of two different products from the reaction of Ig-i with nuclear complexes but only one with cytosol complexes suggests that the nuclear form of the receptor may contain more immunochemical determinants per molecule than are present or accessible in the extranuclear form.…”

Section: Discussionmentioning

confidence: 80%

Antibodies to estrogen receptor: Immunochemical similarity of estrophilin from various mammalian species

Greene

Closs

Fleming

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

130

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Immunoglobulin obtained from the serum of rabbits immunized with a highly purified preparation of estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to the receptor protein (estrophilin) The action of estrogenic hormones in stimulating growth and function of female reproductive tissues takes place primarily, though perhaps not exclusively, through their interaction with an extranuclear receptor protein (estrophilin) present in characteristic amounts in hormone-dependent or "target" cells (1-3). Association with the steroid hormone induces conversion of the receptor to an active form; the activated hormone-receptor complex is translocated to the nucleus where it binds in the chromatin and in some way enhances the ability of the nucleus to synthesize certain types of RNA.Despite recent advances in our knowledge about the intracellular interactions of steroid-receptor complexes, detailed understanding of the processes of receptor activation, translocation, and nuclear binding is still far from complete. In the hope that techniques of immunochemistry might provide new approaches to the study of hormone receptors, we have prepared specific antibodies to estrophilin by immunizing rabbits with a highly purified preparation of the estradiol-receptor complex (ER) from calf uterine nuclei. This paper describes evidence for the presence of these antibodies in the serum of the immunized rabbits and for their crossreactivity with hormone-receptor complexes from various sources.MATERIALS AND METHODS Reagents. [6,7-3H]Estradiol-17f (57 Ci/mmol) and [2,4,6,7-3H]estradiol-17fl (108 Ci/mmol) were obtained from

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Section: Discussionmentioning

confidence: 80%

Antibodies to estrogen receptor: Immunochemical similarity of estrophilin from various mammalian species

Greene

Closs

Fleming

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

130

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“…The presence of specific protein receptors for oestradiol in the uteri of many species is now well established (Gorski, Toft, Shyamala, Smith & Notides, 1968;Jensen, Suzuki, Numata, Smith & DeSombre, 1969). At least two distinct types of specific receptor have been identified, one in the cytoplasm and the other in the nucleus of the cell (Toft & Gorski, 1966;Shyamala & Gorski, 1967).…”

mentioning

confidence: 99%

Changes in the concentration of high-affinity oestradiol receptors in rat uterine supernatant preparations during the oestrous cycle, pseudopregnancy, pregnancy, maturation and after ovariectomy

Feherty

Robertson

Waynforth

et al. 1970

Biochemical Journal

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A quantitative method was used to determine the concentration of high-affinity oestradiol-receptor sites in rat uterine supernatant preparations under various physiological conditions. Cyclic changes in concentration were observed during the oestrous cycle, with a maximum occurring in late dioestrus. The changes followed a similar pattern in endometrium and myometrium, although concentrations were higher in the former. In pseudopregnancy the concentration was initially low, rising to a maximum on the tenth day. In early pregnancy a high concentration of receptor was found to be associated with the developing placenta, but this declined in later stages of pregnancy. After ovariectomy or combined ovariectomy and adrenalectomy the receptor concentration remained at a constant low value that could be increased by treatment with oestradiol. The receptor concentration was considerably higher in immature than in adult uteri.

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“…The hormones first bind to a specific receptor protein present in cytoplasm (Yamamoto & Alberts, 1976) and then these hormone receptor complexes bind to cellular DNA (Jensen et al, 1968;Gorski et al, 1968). This interaction has significant effects on synthesis and translation of host cell mRNA (Higgins & Gehring, 1978).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Replication of Human Cytomegalovirus in Human Fibroblasts Treated with Dexamethasone

Tanaka

Ogura

Kamiya

et al. 1984

Journal of General Virology

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SUMMARYThe effect of glucocorticoid hormones on the replication of human cytomegalovirus (HCMV) was studied in human embryonic lung (HEL) cells. Treatment of cells with pharmacological concentrations of adrenal glucocorticoids such as dexamethasone enhanced HCMV replication; treatment with oestrogenic or androgenic hormones did not do so. In dexamethasone-treated HEL cells there was an approximately tenfold increase in virus yield, with the virus eclipse period shortened by 1 day compared to control cultures. Treatment of cells with the hormone also enhanced plaquing efficiency of the virus by approximately tenfold. As the synthesis of virus-specific immediate early proteins and antigens was notably enhanced together with an increase of HCMV DNA synthesis, it appeared that the early stages of the HCMV replication cycle might be under hormonal control. Moreover, the data presented suggest that the hormonal enhancement of HCMV replication involves specific receptor proteins and requires the synthesis of a specific cellular mRNA(s).

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Hormone Receptors: Studies on the Interaction of Estrogen with the Uterus

Cited by 250 publications

References 10 publications

Antibodies to estrogen receptor: Immunochemical similarity of estrophilin from various mammalian species

Antibodies to estrogen receptor: Immunochemical similarity of estrophilin from various mammalian species

Changes in the concentration of high-affinity oestradiol receptors in rat uterine supernatant preparations during the oestrous cycle, pseudopregnancy, pregnancy, maturation and after ovariectomy

Enhanced Replication of Human Cytomegalovirus in Human Fibroblasts Treated with Dexamethasone

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