Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis

Feng, Wei; Chen, Zheng; Ren, Xiaofang; Tian, Ye; Wang, Fucheng; Liu, Chao; Jin, Pengcheng; Li, Zongyue; Zhang, Feixiong; Zhu, Beiwei

doi:10.1530/rep-16-0484

Cited by 28 publications

(23 citation statements)

References 54 publications

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“…Several studies have reported that the expression of proteins involved in lipid metabolism can affect testicular function in mice (Wang et al, 2017;Sharma et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Yang

Bae

Song

et al. 2020

J Anim Reprod Biotechnol

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A high-cholesterol diet can reduce male fertility. However, it is not known whether a high-cholesterol diet can regulate the expression of genes involved in sperm maturation and sperm fertilizing ability. Quercetin, a natural product, is known to have cytoprotective effects by regulating lipid metabolism in various cell types. This study aimed to confirm the expression of genes involved in sperm maturation in the testes of mice fed a high-cholesterol diet and to determine whether quercetin can reverse the genetic regulation of cholesterol. Mice were divided into groups fed a normal chow diet and a high-cholesterol diet. Mice fed the high-cholesterol diet were dose-dependently supplemented with quercetin for 6 weeks. Investigations using quantitative PCR and in situ hybridization revealed that the high-cholesterol diet alters the expression of genes associated with sperm maturation in the testes of mice, and this was reversed with the supplementation of quercetin. In addition, the high-cholesterol diet regulated the expression of genes related to lipid metabolism in the liver of mice. Under a highcholesterol diet, quercetin can improve male fertility by regulating the expression of genes involved in sperm maturation.

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“…Several studies have reported that the expression of proteins involved in lipid metabolism can affect testicular function in mice (Wang et al, 2017;Sharma et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Yang

Bae

Song

et al. 2020

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

show abstract

“…Obese, diabetic and aging women typically suffer from abnormal body metabolism such as hypertension, hyperglycemia and hyperlipidemia [11][12][13][14][15][16], causing long-term stress in oocytes [17], which severely damages the quality of oocytes, thereby leading to lower pregnancy rate [18].…”

Section: Discussionmentioning

confidence: 99%

Regulation of [Ca2+]i Oscillations and Mitochondrial Activity by Various Calcium Transporters in Mouse Oocytes

Wang

Meng

et al. 2020

Preprint

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Oocyte activation inefficiency is one of the reasons for female infertility and Ca 2+ functions play a critical role in the regulation of oocyte activation. We used various inhibitors of Ca 2+ channels and pumps located on the plasma membrane, the endoplasmic reticulum or both, including sarcoplasmic/endoplasmic reticulum Ca 2+ ATPases (SERCAs, the main Ca 2+ pumps which decrease the intracellular Ca 2+ level by reaccumulating Ca 2+ into the sarcoplasmic reticulum), transient receptor potential (TRP) ion channel subfamily member 7 (TRPM7, a Ca 2+ /Mg 2+ -permeable non-selective cation channel), T-type Ca 2+ channels and calcium channel Orai1, to investigate their roles in[Ca 2+ ] i oscillation patterns and mitochondrial membrane potential during oocyte activation by real-time recording. Our results show that SERCAs, TRPM7 and T-type Ca 2+ channels are important for initiation and maintenance of [Ca 2+ ] i oscillations, which is required for mitochondrial membrane potential changes during oocyte activation, as well as for subsequent pronuclear formation and transition to embryo development, while the function of calcium channel Orai1 is not confirmed. Increasing the knowledge of these transporters may provide a theoretical basis for improving oocyte activation in human assisted reproduction clinics.

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“…Cholesterol is the most abundant sterol of membrane properties in sperm cells that have the capacity to inhibit the capacitation [53]. It was reported that spermiogenesis ceased at the elongation phase of HSL-knockout mice [54]. Thus, higher abundance of HSL in ram spermatozoa probably represents higher capacity ability.…”

Section: Differences In Spermatozoa Proteins Between Ram and Buckmentioning

confidence: 99%

Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach

et al. 2020

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Fresh semen is most commonly used in an artificial insemination of small ruminants, because of low fertility rates of frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep and goats are classified as one species. In order to optimize sperm cryopreservation protocols in sheep and goat, differences in sperm proteomes between ram and buck are necessary to investigate, which may contribute to differences in function and fertility of spermatozoa. In the current work, a data-independent acquisition-mass spectrometry proteomic approach was used to characterize and make a comparison of ram (Ovis aries) and buck (Capra hircus) sperm proteomes. A total of 2,109 proteins were identified in ram and buck spermatozoa, with 238 differentially abundant proteins. Proteins identified in ram and buck spermatozoa are mainly involved in metabolic pathways for generation of energy and diminishing oxidative stress. Specifically, there are greater abundance of spermatozoa proteins related to the immune protective and capacity activities in ram, while protein that inhibit sperm capacitation shows greater abundance in buck. Our results not only provide novel insights into the characteristics and potential activities of spermatozoa proteins, but also expand the potential direction for sperm cryopreservation in ram and buck.

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Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis

Cited by 28 publications

References 54 publications

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Regulation of [Ca2+]i Oscillations and Mitochondrial Activity by Various Calcium Transporters in Mouse Oocytes

Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach

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